Overview

The ITQB/IBET Mass Spectrometry Laboratory performs internal and external services andrelated research work. The laboratory installation process started in October 2002 aiming to be certified as a GLP unit. At the moment we perform mass determination, structural and affinity studies for a broad range of chemical compounds, from small organic and organometalic compounds to peptides, oligosacharides, nucleotides and proteins. The information obtained with the powerful mass spectrometry techniques is fundamental for the structural characterization of chemical and biochemical species. Else than precise mass determination it is possible to perform controlled fragmentation of the molecular ions, which allows to get detailed structural information, like comparative identification of organic compounds, peptide and oligosacharide sequencing, characterization of post-translational modifications: these are the so called MS/MS or tandem mass spectrometry experiments. The mass spectrometry results are important, for example, in monitoring chemical synthesis, purification and identification of chemical and biochemical compounds, metabolic and kinetic studies.
Complex matrices as soils, foodstuff, biological fluids or even microorganisms can be used either directly or after a short pre-treatment. The amount of sample needed depends on the ionisation capability of the compound to be studied, but it is usually very small (ng or less).

	Member of RNEM portuguese mass spectrometry network

Coumpounds               - Oganometalics             - Peptides              - Proteins              - Nucleotides            - Oligosacherids            - Organics

Applications -Monitoring Chemical          -Synthesis/Purification Process -Identification -Sequencing -Metabolic/Kinetic Studies -Peptide MassFinger- Printing