[Mini-symposium] On Fungal Biology, from ecology to drug resistance
| When |
29 Apr, 2016
from
11:00 am to 01:00 pm |
|---|---|
| Where | Room 3.02 |
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Mini-symposium on Fungal Biology, from ecology to drug resistance
Driven to discover
11h-12h
Title: New mechanisms of antifungal drug resistance in C. glabrata, unveiled by iTRAQ-based membrane proteomics
Speaker: Miguel Cacho Teixeira
Affiliation: Biological Sciences Research Group iBB - Institute for Bioengineering and Biosciences Department of Bioengineering Instituto Superior Técnico Universidade de Lisboa
Abstract:
Candida glabrata is the second most common cause of candidemia worldwide and presents higher levels of intrinsic drug resistance when compared to most other fungal pathogens. The understanding of the underlying mechanisms is crucial to design more adequate therapeutic strategies.
In that sense, the membrane proteome of C. glabrata cells was analysed using an iTRAQ MS-based approach. Among the up-regulated proteins, the DHA transporters CgTpo1_2 and CgFlr1 were identified as over-expressed in the C. glabrata membrane in response to clotrimazole and flucytosine, respectively. These drug:H+ antiporters, and their paralogs CgTpo1_1 and CgFlr2, were thus identified and characterized as new determinants of antifungal drug resistance, in lab strains and clinical isolates.
12h13h
Title: Unveiling the biology of the fungal family Botryosphaeriaceae
Speaker: Artur Alves
Affiliation: Investigador Principal, Departamento de Biologia & CESAM, Universidade de Aveiro
Abstract:
Fungi in the family Botryosphaeriaceae (Ascomycota) are well-known endophytes or latent pathogens of woody plant hosts. Although less frequently some species have also been reported to cause infections in humans. In the last 15 years considerable attention has been devoted to these fungi mostly addressing their systematics and phylogenetic relationships, which are key issues for diseases diagnosis. Despite the environmental and economical relevance of these fungi there are several aspects concerning their biology that are still poorly understood. The application of genomics, proteomics and metabolomics tools to the study of Botryosphaeriaceae, although in its infancy, is helping us to elucidate aspects related to their diversity, ecology, pathogenicity, and host-pathogen-environment interaction.
