MPSI : Development and application of high throughput methods for structural and functional studies of membrane proteins.

ITQB Seminar

Title: MPSI : Development and application of high throughput methods for structural and functional studies of membrane proteins.

Speaker: Dr. Vincent Postis

Afilliation: University of Leeds

Host: Margarida Archer

          Membrane Protein Crystallography Laboratory Macromolecular Crystallography Unit

Abstrat:
  The Membrane Protein Structure Initiative (MPSI) is a consortium 
formed by membrane protein research groups in order to develop strategies for 
high throughput (HTP) expression, purification, characterisation,
crystallisation and structure determination of selected integral
membrane
proteins. MPSI brings together the universities of Glasgow, Leeds,
Manchester, Oxford, London (Imperial and Birkbeck), Sheffield and 

CCLRC Daresbury Laboratory. The overall aims include development of
roboticsand recombinational cloning strategies for HTP production of expression 
constructs, development of HTP screening methods for expression, 
solubilisation, purification and folding, with subsequent production 
of large numbers of purified proteins for crystallisation and
structure determination. 
A reliable and cost effective pipeline has been developed for rapid 
and efficient cloning and expression. A high-throughput stability
assay for improvement of detergent and conditions screening (pH, 
glycerol...) before crystallisation has also been implemented.
These various techniques have allowed us to increase the number of
targets giving crystals.