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Methodologies


Mammalian and insect cell lines (CHO, BHK, 293, SP2/0, TE Fly, NIH 3T3, HT1080, PG13, PA317, Sf-9, Sf-21, BAE, BUEC, Caco-2, Caki-2)


Primary cell cultures – alternative model systems for pharmaceutical studies (Brain Cells: neurons and glia, hepatocytes, endothelial), telomerase immortalization


Cell Immobilization


Cell Bank Management


Metabolite quantification
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Microscopic Techniques and Morphologic Analysis
 
 
Fluorescence Techniques
 
 
 
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Quantitative PCR
 
Mycoplasma Detection (PCR e ELISA)
 
Lipid Analysis (phospholipids, cholesterol)
 
Protein Characterization (SDS-PAGE, Western immunoblot, ELISA)
 

 

 
 
Virus Purification (Baculovirus, Adenovirus, Retrovirus)
 
 
 
Virus Titration (Baculovirus, Adenovirus, Retrovirus)
 
 

 



Liquid Chromatography and HPLC (Affinity, Ion Exchange, Size Exclusion)
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