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Brain Research

  1. Simão D, Terrasso AP, Teixeira AP, Brito C, Sonnewald U, Alves PM .  2016.  Functional metabolic interactions of human neuron-astrocyte 3D in vitro networks.  Scientific Reports .  6, 33285.  http://dx.doi.org/10.1038/srep33285
  2. Kleiderman S, Gutbier S, Ugur Tufekci K, Ortega F, Sá JV, Teixeira AP, Brito C, Glaab E, Berninger B, Alves PM, Leist M. 2016. Conversion of Nonproliferating Astrocytes into Neurogenic Neural Stem Cells: Control by FGF2 and Interferon-γ.  Stem Cells. https://doi.org/10.1002/stem.2483
  3. Terrasso AP, Silva AC, Filipe A, Pedroso P, Ferreira AL, Alves PM, Brito C.  2016.  Human neuron-astrocyte 3D co-culture-based assay for evaluation of neuroprotective compounds.  J Pharmacol Toxicol Methods.  83:72-79. https://doi.org/10.1016/j.vascn
  4. Sá JV, Kleiderman S, Brito C, Sonnewald U, Leist M, Teixeira AP, Alves PM.  2016.  Quantification of Metabolic Rearrangements During Neural Stem Cells Differentiation into Astrocytes by Metabolic Flux Analysis.  Neurochemical Research.  ahead of print .  http://dx.doi.org/10.1007/s11064-016-1907-z
  5. Simao D, Pinto C, Fernandes P, Piersanti S, Saggio I, Collinson LM, Schiavo G, Kremer EJ, Alves PM & Brito C. 2016. Evaluation of helper-dependent canine adenovirus vectors in a 3D human CNS model. Gene Therapy, 23(1):86-94 http://dx.doi.org/10.1038/gt.2015.75 
  6. Almeida AS, Soares NL, Vieira M, Gramsbergen JB, Vieira HL. 2016. Carbon Monoxide Releasing Molecule-A1 (CORM-A1) Improves Neurogenesis: Increase of Neuronal Differentiation Yield by Preventing Cell Death.  PLoS One.  11(5):e0154781.  https://doi.org/10.1371/journal.pone.0154781
  7. Materne EM, Ramme AP, Terrasso AP, Serra M, Alves PM, Brito C, Sakharov DA, Tonevitsky AG, Lauster R, Marx U. 2015. A multi-organ chip co-culture of neurospheres and liver equivalents for long-term substance testing. J Biotechnol. Feb 9. pii: S0168-1656(15)00045-0. http://dx.doi.org/10.1016/j.jbiotec.2015.02.002 
  8. Terrasso AP, Pinto C, Serra M, Filipe A, Almeida S, Ferreira AL, Pedroso P, Brito C, Alves PM. Novel scalable 3D cell based model for in vitro neurotoxicity testing: Combining human differentiated neurospheres with gene expression and functional endpoints. J Biotechnol. 2015 Jan 5. pii: S0168-1656(14)01047-5. http://dx.doi.org/10.1016/j.jbiotec.2014.12.011
  9. Gualda EJ, Simão D, Pinto C, Alves PM, Brito C. 2014. Imaging of human differentiated 3D neural aggregates using light sheet fluorescence. Frontiers in Cellular Neuroscience, 8:221. http://dx.doi.org/10.3389/fncel.2014.00221
  10. Almeida AS, Queiroga CS, Sousa MF, Alves PM, Vieira HL, 2012. Carbon monoxide modulates apoptosis by reinforcing oxidative metabolism in astrocytes: role of BCL-2. J Biol Chem, 287 (14): 10761-70. http://dx.doi.org/10.1074/jbc.M111.306738
  11. Brito C, Simão D, Costa I, Malpique R, Pereira CI, Fernandes P, Serra M, Schwarz SC, Schwarz J, Kremer EJ, Alves PM, 2012. Generation and genetic modification of 3D cultures of human dopaminergic neurons derived from neural progenitor cells. Methods, 56(3):452-60. http://dx.doi.org/10.1016/j.ymeth.2012.03.005
  12. Queiroga CS, Tomasi S, Wideroe M, Alves PM, Vercelli A, Vieira HL, 2012. Preconditioning triggered by Carbon Monoxide (CO) provides neuronal protection following perinatal hypoxia-ischemia. Plos One, 7(8): e42632. http://dx.doi.org/10.1371/journal.pone.0042632
  13. Vieira HL, Alves PM, Vercelli A, 2011. Modulation of neuronal stem cell differentiation by hypoxia and reactive oxygen species. Prog Neurobiol, 93:444-55. http://dx.doi.org/10.1016/j.pneurobio.2011.01.007
  14. Santos SS, Sonnewald U, Carrondo MJ, Alves PM, 2011. The role of glia in neuronal recovery following anoxia: in vitro evidence of neuronal adaptation. Neurochem Int, 58:665-75. http://dx.doi.org/10.1016/j.neuint.2011.02.005
  15. Amaral AI , Teixeira AP, Sonnewald U, Alves PM, 2011. Estimation of intracellular fluxes in cerebellar neurons after hypoglycemia: importance of the pyruvate recycling pathway and glutamine oxidation. J Neurosci Res, 89(5)-700:10. http://dx.doi.org/10.1002/jnr.22571
  16. Amaral IA, Teixeira AP, Håkonsen BI, Sonnewald U, Alves PM, 2011. A comprehensive metabolic profile of cultured astrocytes using isotopic transient metabolic flux analysis and 13C-labelled glucose. Front Neuroenerg, 3:5. http://dx.doi.org/10.3389/fnene.2011.00005
  17. Amaral AI, Teixeira AP, Martens S, Sousa MF, Bernal V, Alves PM, 2010. Metabolic alterations induced by ischemia in primary cultures of astrocytes: merging 13C NMR spectroscopy and metabolic flux analysis. J Neurochem, 113(3):735-48. http://dx.doi.org/10.1111/j.1471-4159.2010.06636.x
  18. Serra M, Brito C, Costa E, Sousa MF, Alves PM, 2009. Integrating human stem cell expansion and neuronal differentiation in bioreactors. BMC Biotechnol, 9:82. http://dx.doi.org/10.1186/1472-6750-9-82
  19. Vieira HL, Queiroga CS, Alves PM, 2008. Preconditioning induced by Carbon Monoxide provides neuronal protection against apoptosis. J Neurochem, 107:375-84. http://dx.doi.org/10.1111/j.1471-4159.2008.05610.x
  20. Teixeira AP, Santos SS, Carinhas N, Oliveira R, Alves PM, 2008. Combining metabolic flux analysis tools and 13C-NMR to estimate intracellular fluxes of cultured astrocytes. Neurochem Int, 52:478-86. http://dx.doi.org/10.1016/j.neuint.2007.08.007
  21. Santos SS, Leite SB, Sonnewald U, Carrondo MJ, Alves PM, 2007. Stirrer vessel cultures of rat brain cells aggregates: characterization of major metabolic pathways and cell population dynamics. J Neurosci Res, 85(15):3386-97. http://dx.doi.org/10.1002/jnr.21409
  22. Serra M, Leite SB, Brito C, Costa J, Carrondo MJ, Alves PM, 2007. Novel culture strategy for human stem cell proliferation and neuronal differentiation. J Neurosci Res, 85(16):3557-66. http://dx.doi.org/10.1002/jnr.21451
  23. Santos SS, Gibson GE, Cooper AJ, Denton TT, Thompson CM, Bunik VI, Alves PM, Sonnewald U, 2006. Inhibitors of the a-ketoglutarate dehydrogenase complex alter [1-13C]Glucose and [U-13C]Glutamate metabolism in cerebellar granule neurons. J Neurosci Res, 83:450-8. http://dx.doi.org/10.1002/jnr.20749
  24. Fonseca LL, Monteiro MA, Alves PM, Carrondo MJ, Santos H, 2005. Cultures of rat astrocytes challenged with a steady supply of glutamate: a new model to study flux distribution in the glutamate-glutamine cycle. Glia, 51:286-96. http://dx.doi.org/10.1002/glia.20209
  25. Santos SS, Fonseca LL, Monteiro MA, Carrondo MJ, Alves PM,  2005. Culturing primary brain astrocytes under fully controlled environment in a novel bioreactor. J Neurosci Res, 79:26-32. http://dx.doi.org/10.1002/jnr.20279
  26. Alves PM, Fonseca LL, Peixoto CC, Almeida AC, Carrondo MJ, Santos H, 2000. NMR studies on energy metabolism of immobilized primary neurons and astrocytes during hypoxia, ischemia and hypoglycemia. NMR Biomed, 13:438-48. http://dx.doi.org/10.1002/nbm.665
  27. Alves PM, Nunes R, Zhang C, Maycock CD, Sonnewald U, Carrondo MJ, Santos H, 2000. Metabolism of 3-[13C]malate in primary cultures of mouse astrocytes. Dev Neurosci, 22:456-62. http://dx.doi.org/10.1159/000017475
  28. Alves PM, Moreira JL, Rodrigues JM, Aunins JG, Carrondo MJ, 1996. Immobilization of primary astrocytes and neurons for on-line monitoring of biochemical processes by NMR. Dev Neurosci, 18:478-83. http://dx.doi.org/10.1159/000111443
  29. Alves PM, MC Mckenna, U Sonnewald, 1995. Lactate metabolism in mouse brain astrocytes studied by [13C]NMR spectroscopy. NeuroReport, 6:2201-04. http://www.ncbi.nlm.nih.gov/pubmed/8595202
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