Methodologies
Methods most commonly used at GPlantS
- DNA and RNA extraction
- PCR and Real-Time qPCR)
- RNA-seq analysis (e.g. read mapping, de novo assembly, differential expression)
- Yeast-one hybrid and Yeast-two hybrid
- Gene cloning and gene editing (e.g. CRISPR/Cas)
- Protoplast isolation and transformation
- Agrobacterium mediated genetic transformation
- Particle bombardment for plant transformation
- Proteomic analysis (e.g. SDS-PAGE, 2-DE)
- In vitro molecular analysis (e.g. Pull-down, Co-IP)
- Imaging techniques (e.g. BiFC)
- Molecular cytogenetics (e.g. FISH)
- Plant physiology
