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Research

 

Research 

 

Selected Publications                     

Staphylococcus aureus       Fighting the innate immune system


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Lobo SAL, Scott A, Videira MAM, Winpenny D, Gardner M, Palmer MJ,  Schroeder S,  Lawrence A, Parkinson T, Warren MJ, and Saraiva LM (2015) Staphylococcus aureus haem biosynthesis: characterisation of the enzymes involved in final steps of the pathway. Mol. Microbiol., 97(3):472-87.

Nobre LS and Saraiva LM. (2013) Effect of combined oxidative and nitrosative stresses on Staphylococcus aureus transcriptome. Appl. Microbiol. Biotechnol. 97, 2563-73.

Nobre LS, Todorovic S, Tavares AFN, Oldfield P, Hildebrandt P, Teixeira M, and Saraiva, LM (2010) Binding of azole antibiotics to Staphylococcus aureus flavohemoglobin increases intracellular oxidative stress, J. Bacteriol. 192, 1527-33.

Tavares AFN, Nobre LS, Melo AMP, and Saraiva LM. (2009) A novel nitroreductase of Staphylococcus aureus with S-nitrosoglutathione reductase activity. J. Bacteriol. 191, 3403-3406.

   

       

Helicobacter species               Living in a nitrite rich environment

 

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Flint A, Stintzi A  Saraiva LM (2016) Oxidative and nitrosative stress defences of Helicobacter and Campylobacter species that counteract mammalian immunity. FEMS Microbiol Rev., in press.

Parente MR, Monteiro JT, Martins GG, and Saraiva LM (2016) Helicobacter pullorum induces in murine macrophages nitric oxide release that promotes phagocytosis and killing, Microbiology. 162:503-12

Justino MC, Parente MR, Boneca IG, and Saraiva LM (2014) FrxA is a GSNO reductase enzyme that contributes to Helicobacter pylori pathogenicity. FEBS J. 281, 4495-4505.

Justino MC, Ecobichon C, Fernandes AF, Boneca IG, Saraiva LM. (2012) Helicobacter pylori has an unprecedented nitric oxide detoxifying system. Antioxid. Redox. Signal. 17, 1190-200.

 

Staphylococcus aureus 

Desulfovibrio spp.

Heme biosynthesis      

Desulfovibrio.jpg           

     

        

Lobo SAL, Scott A, Videira MAM, Winpenny D, Gardner M, Palmer MJ,  Schroeder S,  Lawrence A, Parkinson T, Warren MJ, and Saraiva LM (2015) Staphylococcus aureus haem biosynthesis: characterisation of the enzymes involved in final steps of the pathway. Mol. Microbiol., 97(3):472-87.

Lobo SAL, Lawrence AD, Romão CV, Warren MJ, Teixeira M, and Saraiva LM (2014) Characterization of Desulfovibrio vulgaris haem b synthase, a radical SAM family member. BBA Proteins and Proteomics, 1844, 1238-47.

Bali S, Lawrence AD, Lobo SA, Saraiva LM, Golding BT, Palmer DJ, Howard MJ, Ferguson SJ and Warren MJ. (2011) Molecular hijacking of siroheme for the synthesis of heme and d1 heme. Proc. Natl. Acad. Sci USA, 108, 18206-5.

Romão CV, Ladakis D, Lobo SA, Carrondo MA, Brindley AA, Deery E, Matias PM, Pickersgill MW, Saraiva LM and Warren MJ (2011) Evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization. Proc. Natl. Acad. Sci. USA.108, 97-102.

 

Escherichia coli                  Nitrosative/oxidative stress defences against

                                            mammalian macrophages

 

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Nobre LS,  Meloni D, Teixeira M, Viscogliosi E, and Saraiva LM (2016) Trichomonas vaginalis Repair of Iron Centres proteins: the different role of two paralogs. Protist. 167, 222–233. 

Nobre LS, Lousa D,  Pacheco I, Soares CM, Teixeira M and Saraiva LM (2015)  Insights into the structure of the diiron site of RIC from Escherichia coli. FEBS Lett. 589 (4):426-431.

Nobre LS, Garcia-Serres R, Todorovic S, Hildebrandt P, Teixeira M,  Latour JM,  and Saraiva LM. (2014) Escherichia coli RIC is able to donate iron to iron-sulfur clusters. PLOS One , 16, 9(4), e95222.

Justino MC, Almeida CC, Teixeira M, and Saraiva LM. (2007) Escherichia coli di-iron YtfE protein is necessary for the repair of stress damaged iron-sulfur clusters. J. Biol. Chem. 282, 10352-10359.

 

CORMs 

 

 

New antibiotics

 

Nobre LS, Jeremias H, Romão CC and Saraiva LM (2016) Examining the antimicrobial activity and toxicity to animal cells of different types of CO-releasing molecules,  Dalton Trans. 45,1455-66.

Tavares AF, Parente MR, Justino MC, Oleastro M, Nobre LS, and Saraiva LM. (2013) The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori. PLoS One. 8(12):e83157.

Tavares AF, Teixeira M, Romao CC, Seixas JD, Nobre LS, and Saraiva LM. (2011) Reactive oxygen species mediate bactericidal killing elicited by carbon monoxide-releasing molecules. J. Biol Chem. 286, 26708-17. 

Nobre LS, Seixas JD, Romão CC, and Saraiva LM. (2007) The antimicrobial action of carbon monoxide releasing compounds. Antimicrob. Agents Chemother. 51, 4303-07.

     

    

 


Keywords

 

           

Staphylococcus aureus, Helicobacter pylori, Desulfovibrio, sulphate-reducing bacteria, Escherichia coli;

Bacteria, anaerobic microorganisms, pathogen, antibiotic, antibiotic resistance, immune system, carbon monoxide, iron metabolism, heme;

Cell culture, molecular genetics, genomics, transcriptomics, microbiology, biochemistry.

 

 

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