Miguel R. Guerreiro | PhD in Biotechnology | Biosensors, Viral Vectors, Cell Line Development, Molecular Biology.

Miguel R. Guerreiro

Scientist at iBET, developing innovative cell-based biosensing strategies

About Me

I have always been interested in the fields of biotechnology and bioengineering with a strong component on the applicability of my research outputs. As such, I started to work in mammalian cell line development and viral vector engineering for gene therapy and vaccination applications. My research focus has now been shifted towards the development of innovative cell-based biosensing strategies for detection and quantification of clinical and biotechnological important cellular and viral events.

I have gained multiple competences and skills in the fields of cell line development, virology, vectorology, molecular biology, biosensors, cell-based assays, synthetic biology, and protein engineering.

In parallel, I am actively engaged in tutoring master students and new members of iBET, as well as in science dissemination activities.

Work experience

Ph.D. student in Biotechnology - Instituto de Biologia Experimental e Tecnológica (APR 2013 - PRESENT)

Development of cell-based switch-on biosensors for detection and quantification of label-free viruses. Supervision: Dr. Ana Sofia Coroadinha.

Guest Researcher - Max Planck Institute for Dynamics of Complex Technical System (FEB 2013 - APR 2013)

Measurement of key metabolic enzyme activities in HEK293 cells using rapid and sensitive microplate-based assays. Supervision: Dr. Yvonne Genzel.

Research Fellow - Instituto de Biologia Experimental e Tecnológica (MAY 2011 - MAR 2013)

M.Sc. student - Instituto de Biologia Experimental e Tecnológica (SEP 2010 - SEP 2011)

Manipulation of Warburg Effect for the improvement of complex biopharmaceuticals production in cultured mammalian cells.

Research Trainee - Instituto de Biologia Experimental e Tecnológica (DEC 2008 - NOV 2009)

Improving the potential of retroviral-like particles as candidate vaccines for Hepatitis C by changing the composition of host-derived immunogens.

Publications

  • (7) Tomás, H.A.; Mestre, D.A.; Rodrigues, A.F.; Guerreiro, M.R.; Carrondo, M.J.T.; Coroadinha, A.S. Improved GaLV-TR glycoproteins to pseudotype lentiviral vectors: impact of viral protease activity in the production of LV pseudotypes. Mol. Ther. - Methods Clin. Dev. 2019, 15, 1-8. DOI: 10.1016/j.omtm.2019.08.001
  • (6) Guerreiro, M.R.; Freitas, D.F.; Alves, P.M.; Coroadinha, A.S. Detection and Quantification of Label-Free Infectious Adenovirus Using a Switch-On Cell-Based Fluorescent Biosensor. ACS Sensors 2019, 4, 1654-1661. DOI: 10.1021/acssensors.9b00489
  • (5) Rodrigues, A.F.; Guerreiro, M.R.; Formas-Oliveira, A.S.; Fernandes, P.; Blechert, A.K.; Genzel, Y.; Alves, P.M.; Hu, W.S.; Coroadinha, A.S. Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK. Biotechnol. Bioeng. 2016, 113, 150-162. DOI: 10.1002/bit.25691
  • (4) Rodrigues, A.F.; Soares, H.R.; Guerreiro, M.R.; Alves, P.M.; Coroadinha, A.S. Viral vaccines and their manufacturing cell substrates: New trends and designs in modern vaccinology. Biotechnol. J. 2015, 10, 1329-1344. DOI: 10.1002/biot.201400387
  • (3) Rodrigues, A.F.; Formas-Oliveira, A.S.; Guerreiro, M.R.; Tomás, H.A.; Alves, P.M.; Coroadinha, A.S. Single-step cloning-screening method: A new tool for developing and studying high-titer viral vector producer cells. Gene Ther. 2015, 22, 685-695. DOI: 10.1038/gt.2015.44
  • (2) Fernandes, P.; Simão, D.; Guerreiro, M.R.; Kremer, E.J.; Coroadinha, A.S.; Alves, P.M. Impact of adenovirus life cycle progression on the generation of canine helper-dependent vectors. Gene Ther. 2015, 22, 40-49. DOI: 10.1038/gt.2014.92
  • (1) Rodrigues, A.F.; Guerreiro, M.R.; Santiago, V.M.; Dalba, C.; Klatzmann, D.; Alves, P.M.; Carrondo, M.J.T.; Coroadinha, A.S. Down-regulation of CD81 tetraspanin in human cells producing retroviral-based particles: Tailoring vector composition. Biotechnol. Bioeng. 2011, 108, 2623-2633. DOI: 10.1002/bit.23231
  • (1) Rodrigues, A.F.; Guerreiro, M.R.; Castro, R; Tomás, H.A.; Dalba, C; Klatzmann, D; Alves, P.M.; Carrondo, M.J.T.; Coroadinha, A.S. (2011). Down-regulation of CD81 in human cells producing HCV-E1/E2 retroVLPs. In H. Hauser (Ed.), 22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies. Vienna, Austria. May 15-18, 2011. BMC Proceedings, 5(Suppl 8):P72. DOI: 10.1186/1753-6561-5-S8-P72
  • (2) Guerreiro, M.R.; Alves, P.M.; Coroadinha, A.S. Switch-on fluorescent cell-based biosensors for quantification of label-free virus and viral vectors. 2nd ESACT Frontiers Retreat. Zagreb, Croatia. October 17-19, 2018.
  • (1) Guerreiro, M.R.; Alves, P.M.; Coroadinha, A.S. Fluorescent cell-based biosensors for detection and quantification of label-free virus and viral vectors. Poster Spotlight at the 25th Meeting of the European Society for Animal Cell Technology. Lausanne, Switzerland. May 14-17, 2017.
  • (18) Guerreiro, M.R.; Freitas, D.F.; Alves, P.M.; Coroadinha, A.S. (May 5-8, 2019) Biosensors for quantification of infectious label-free viruses. 26th Meeting of the European Society for Animal Cell Technology. Copenhagen, Denmark.
  • (17) Guerreiro, M.R.; Alves, P.M.; Coroadinha, A.S. (May 14-17, 2017) Fluorescent cell-based biosensors for detection and quantification of label-free virus and viral vectors. 25th Meeting of the European Society for Animal Cell Technology. Lausanne, Switzerland.
  • (16) Guerreiro, M.R.; Rodrigues, A.F.; Alves, P.M.; Coroadinha, A.S. (October 19-22, 2016) Cell-based biosensors for detection and quantification of label-free virus and viral vectors for research and diagnostics. European Congress of Virology 2016. Hamburg, Germany.
  • (15) Guerreiro, M.R.; Rodrigues, A.F.; Alves, P.M.; Coroadinha, A.S. (September 4-7, 2016) Cell-based biosensors for detection and quantification of label-free virus for research and diagnostics. 10th Vaccine Congress. Amsterdam, The Netherlands.
  • (14) Guerreiro, M.R.; Rodrigues, A.F.; Alves, P.M.; Coroadinha, A.S. (September 17-20, 2015). Cell-based biosensors for detection and quantification of gene therapy label-free viral vectors. ESGCT and FSGT Collaborative Congress 2015. Helsinki, Finland.
  • (13) Guerreiro, M.R.; Rodrigues, A.F.; Formas-Oliveira, A.S.; Fernandes, P.; Blechert, A.-K.; Genzel, Y.; Alves, P.M.; Hu, W.S.; Coroadinha, A.S. (May 31-June 3, 2015). Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK. 24th Meeting of the European Society for Animal Cell Technology. Barcelona, Spain.
  • (12) Soares, H.R.; Castro, R.; Tomás, H.; Rodrigues, A.F.; Almeida, A.I.; Guerreiro, M.R.; Bellier, B.; Klatzmann, D.; Alves, P.M.; Carrondo, M.J.T.; Coroadinha, A.S. (June 8-13, 2014). Development of a candidate vaccine for Hepatitis C Virus. Vaccine Technology V. Playa del Carmen, Mexico.
  • (11) Rodrigues, A.F.; Oliveira, A.S.; Guerreiro, M.R.; Tomás, H.; Alves, P.M.; Carrondo, M.J.T.; Coroadinha, A.S. (October 25-28, 2013). Single step cloning-titration method: accelerating the development and engineering of high-titer retro and lentiviral vector producer cell lines. ESGCT and SETGyC Collaborative Congress. Madrid, Spain.
  • (10) Rodrigues, A.F.; Oliveira, A.S.; Guerreiro, M.R.; Tomás, H.; Alves, P.M.; Blechert, A.-K.; Genzel, Y.; Coroadinha, A.S. (June 23-26, 2013). Engineering the energy metabolism and lactate production in mammalian cells: down-regulation of the Warburg effect. 23rd European Society for Animal Cell Technology Meeting: Better Cells for Better Health. Lille, France.
  • (9) Rodrigues, A.F.; Oliveira, A.S.; Guerreiro, M.R.; Tomás, H.; Alves, P.M.; Coroadinha, A.S. (June 23-26, 2013). Single step cloning-titration method: accelerating the development and engineering of high-titer virus producing cell lines. 23rd European Society for Animal Cell Technology Meeting: Better Cells for Better Health. Lille, France.
  • (8) Blechert, A.-K.; Rath, A.; Sann, H.; Spaner, D.; Guerreiro, M.R.; Coroadinha, A.S.; Genzel, Y.; Reichl, U. (June 23-26, 2013). Towards an analytical platform for metabolic studies in adherent and suspension cells: intracellular metabolite concentrations and maximum enzyme activities. 23rd European Society for Animal Cell Technology Meeting: Better Cells for Better Health. Lille, France.
  • (7) Fernandes, P.; Monteiro, F.; Guerreiro, M.R.; Kremer, E.J.; Coroadinha, A.S.; Alves, P.M. (June 23-26, 2013). The role of adenovirus replication steps on the production of helper-dependent vectors. 23rd European Society for Animal Cell Technology Meeting: Better Cells for Better Health. Lille, France.
  • (6) Soares, H.R.; Castro, R.; Tomás, H.; Rodrigues, A.F.; Almeida, A.I.; Guerreiro, M.R.; Bellier, B.; Klatzmann, D.; Alves, P.M.; Carrondo, M.J.T.; Coroadinha, A.S. (June 23-26, 2013). Improved HEK293-FLEX cells for a better HCV vaccine candidate. 23rd European Society for Animal Cell Technology Meeting: Better Cells for Better Health. Lille, France.
  • (5) Soares, H.R.; Castro, R.; Rodrigues, A.F.; Guerreiro, M.R.; Alves, P.M.; Carrondo, M.J.T.; Klatzmann, D.; Bellier, B.; Coroadinha A.S. (August 19-21, 2012). HCV vaccine candidate optimization: avoid breaking immune tolerance. Cell Symposia Human Immunity. Lisboa, Portugal.
  • (4) Rodrigues, A.F.; Guerreiro, M.R.; Fernandes, P.; Alves, P.M.; Hu, W.S.; Coroadinha, A.S. (April 22-27, 2012). Engineering the energy metabolism and lactate production in mammalian cells producing complex biopharmaceuticals: down-regulation of the Warburg effect. Cell Culture Engineering XIII. Scottsdale, Arizona, USA.
  • (3) Rodrigues, A.F.; Guerreiro, M.R.; Castro, R.; Fluckiger, A.C.; Dalba, C.; Klatzmann, D.; Alves, P.M.; Carrondo, M.J.T.; Coroadinha, A.S. (May 15-18, 2011). Down-regulation of CD81 in packaging cells producing HCV-E1/E2 retroVLPs: tailoring retroviral vector immunotoxicity. 22nd European Society for Animal Cell Technology Meeting on Cell Based Technologies. Vienna, Austria.
  • (2) Rodrigues, A.F.; Guerreiro, M.R.; Castro, R.; Fluckiger, A.C.; Dalba, C.; Klatzmann, D.; Alves, P.M.; Carrondo, M.J.T.; Coroadinha, A.S. (April 7-8, 2011). Down-regulation of CD81 in packaging cells producing HCV-E1/E2 retroVLPs. CliniGene Network of Excellence (NoE): Clinical Gene transfer: state of the art conference. Paris, France.
  • (1) Rodrigues, A.F.; Guerreiro, M.R.; Santiago, V.; Dalba, C.; Klatzmann, D.; Alves, P.M.; Carrondo, M.J.T.; Coroadinha, A.S. (October 22-25, 2010). Silencing host cell proteins associated with retroviral particles. 18th European Society of Gene and Cell Therapy Annual Congress. Milan, Italy.