Zeiss Elyra PS1

Inverted Super-Resolution Microscope   Laser lines 405 nm;  488 nm;  561 nm   Detectors PCO edge sCMOS camera (SR - SIM)   Available Techniques Z-stacks; Multiple Positions; Time series; Tile scan Structured Illumination Microscopy   Training is required before use  An equipment guide is available in our Guides section Contact BIC for training System Booking Location: 5.25

Warnings

• The equipment cannot be used without official training

• Before your first use contact the responsible people to schedule training

• Never LOOK DIRECTLY into the beam paths as they are capable of permanently damaging the human eye

• Save all data into your own storage device as we routinely clean the computer’s data

• Register your utilization in the logbook

• SIM raw data reconstruction must be done in the microscope computer (book slots accordingly). For all downstream image analysis a free software license is available for ZEN lite at ZEISS website. Alternatively, you can use FIJI.

• Contact the responsible people in case of any doubt or any issue with the equipment

Booking Rules

Booking is done online through Agendo. Please mind the following rules when booking the microscope:

• Maximum 2 slots per user per week (one slot = one morning or one afternoon);
• These rules are applicable until the previous Friday. If you need more than two slots in a week and they are still available on the previous Friday, at 4pm, feel free to book them.

Know Your Fluorophores

Use resources such as the FPBase or the Thermofisher SpectraViewers to explore your fluorophores.  Learn their excitation and emission wavelengths, and what are their optimal laser lines and filters. This is necessary to use the confocal to its full potential.

Brief Description

The ZEISS Elyra PS.1 is a super-resolution microscope that utilizes Structured Illumination Microscopy (SIM) to provide high-resolution imaging capabilities beyond the diffraction limit of conventional light microscopes. 

Suggestion for “Materials and Methods”

Cells were imaged by structured illumination microscopy (SIM) using an Elyra PS.1 microscope (Zeiss) equipped with a Plan-Apochromat 63×/1.4 oil differential interference contrast M27 objective. SIM images were acquired using five grid rotations with 34 µm grating period for the 561 nm laser (100 mW, at 50% maximal power) and 23 µm grating period for the 405 nm laser (100 mW, at 100% maximal power). Images were captured using a PCO Edge 5.5 camera and reconstructed using the software ZEN black v8.1.0.484.

Information to be added to the Acknowledgements section

This work was partially supported by PPBI - Portuguese Platform of BioImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE - "Orçamento de Estado" and by european funds from FEDER - "Fundo Europeu de Desenvolvimento Regional".

Filter Sets 

• 405 EF BP 420-480 / LP 750 SR
• 488 EF BP 495-550 / LP 750 SR
• 561 EF BP 570-620 / LP 750 SR

BP stands for a bandpass filter

LP stands for longpass filter 

Objectives