Zeiss Axio Zoom.V16

Large-field Stereo Microscope

 

Transmitted Light
Bright Field; Dark Field

 

Fluorescence Filters For
GFP; FITC; YFP; CY3; TX2; mCherry

 

Available Techniques
Large field Observation
Biofilm Observation

 

Training is required before use
An equipment guide is available in our Guides section
Contact BIC for training
System Booking
Location: 5.25

 zeiss_axio_zoom_v16.jpg

Warnings

  • Before you get started for the first time in this microscope consult the responsible people for proper training
  • ONLY turn ON the lamp if it has been off for more than one hour
  • Do not turn OFF the lamp if someone else will be using the microscope in the next few hours. To avoid unnecessary working hours or usage constraints, please contact the next user after using the microscope
  • You must turn ON THE LAMP FIRST and then the remaining devices
  • THE LAMP must be the LAST TO TURN OFF
  • Never LOOK DIRECTLY into the beam paths as they are capable of permanently damaging the human eye

 

Brief Description

Axio Zoom.V16 is a fully automated stereo microscope that successfully combines a large field of view, zoom and working distance. It achieves a considerable numerical aperture for a medium zoom range, thereby providing superior fluorescence brightness in large object fields such as biofilms or tissues. This Axio Zoom.V16 is equipped with a 1x zoom objective having a numerical aperture of NA 0.25.

 

Suggestion for description in “Materials and Methods”

Images were acquired on a Zeiss Axio Zoom.V16 stereo microscope equipped with a Zeiss Axiocam 503 mono CCD camera and controlled with the Zeiss Zen 2.1 (blue edition) software, using the 1x 0.25 NA objective, the fluorescence filter sets GFP + mRFP and the Bright Field optics.

 

Suggestion for “Acknowledgements”

This work was partially supported by PPBI - Portuguese Platform of BioImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE - "Orçamento de Estado" and by european funds from FEDER - "Fundo Europeu de Desenvolvimento Regional".

 

Filter Sets

Filter Cube

Excitation Filter

Dichromatic Mirror

Emission Filter

Excitation Colour

Emission Colour

GFP1

BP 470/40

495

BP 525/50

Blue/Cyan

Green

YFP1

BP 500/25

515

BP 535/30

Green

Green/Yellow

mRFP1

BP 572/25

590

BP 629/62

Green/Yellow

Red

1 High efficiency (HE) filter set: These filters are distinguished by particularly steep cut-off edges and extremely high transmittances

 

Objectives

  • Magnification: 1x
  • Zeiss System: PlanNeoFluar Z
  • Class: Achromatic objective
  • Numerical Aperture (A): 0.25
  • Free Working Distance (mm): 56
  • Pixel Size for 0.7x (µm): 6.4857
  • Pixel Size for 11.2x (µm): 0.4054
  • Notice that this microscope is equipped with two eyepieces PL 10x/23 Br. foc. Furthermore, a camera adaptor 60N-C1 1x is also installed. In general, the Axio Zoom.V16 has a motorized zoom from 0.7x to 11.2x

 

Camera

  • Brand: Zeiss®
  • Model: Axiocam 503 mono (D)
  • CCD Technology
  • 1936 x 1460 active pixels
  • 38 Frame Rate @ full resolution (frames per sec)
  • 4.54 x 4.54-pixel size (WxH; µm)