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Redox protein-protein interactions and mechanisms of intermolecular electron transfer

Redox protein-protein interactions and mechanisms of intermolecular electron transfer



Thermodynamics is just half of the story. The other half is kinetics. To study electron transfer kinetics in protein systems one needs to consider the actual process of electron transfer and the protein-protein interactions (complex formation) that occur prior to this electron transfer process. We have done research on these subjects using several protein systems and in collaboration with several experimental groups.


Interaction of [NiFe]-Hydrogenase with cytochrome c3 from D. desulfuricans ATCC 27774

hyd and c3


Representation of the secondary structure of Hydrogenase with all the docking solutions obtained with cytochrome c3 (represented as small coloured spheres).
 
test

Lowest energy complex between c3 and hydrogenase from D. desulfuricans ATCC 27774. The cytochrome c3 is the small molecule that appears on the right hand side. The surfaces of both molecules are coloured accordingly to the electrostatic potential.


Work Reference:

  • Matias, P.M., Soares, C.M., Saraiva, L.M., Coelho, R., Morais, J.,LeGall, J., Carrondo, M.A. (2001) "[NiFe] hydrogenase from Desulfovibrio desulfuricans ATCC 27774: primary sequence determination, crystallographic refinement at 1.8 Å and modelling studies of its interaction withthe tetra-haem cytochrome c3", JBIC, 6, 63-81



Interaction of Rubredoxin:oxygen Oxidoreductase (ROO) with Rubredoxin (rdg) from D. gigas


                Rubredoxin (rdg)

       
                   		                                                  ROO

                                        
Secondary structures and surface potential representations of Rubredoxin and ROO from Desulfovibrio gigas




In the left the lowest energy complex between these two proteins evidences the prefered conformation that Rubredoxin has in the interaction with ROO



Work Reference:

  • Victor, B.L., Vicente, J.B., Rodrigues, R., Oliveira, S., Rodrigues-Pousada, C., Frazão, C., Gomes, C.M., Teixeira, M., Soares, C.M. (2003) "Docking and electron transfer studies between rubredoxin and rubredoxin:oxygen oxidoreductase", JBIC, 8, 475-488



Interaction of type I Cytochrome c3 with type II Cytochrome c3 from D. vulgaris Hildenborough


cytc3 I II

Ribbon representation of the type I cytochrome c3 with all the docking solutions obtained with type II
cytochrome c3 (represented as small coloured spheres)
solution 1
          The lowest energy complex obtained
                       by rigid body docking
solution 2
    The second lowest energy complex obtained
                         by rigid body docking



Work Reference:

  • Teixeira, V.H., Baptista, A.M., Soares, C.M. (2004) " Modelling electron transfer thermodynamics in protein complexes: interaction between two cytochromes c3, Biophys.J., 86, 2773-2785



Other systems studied:

  • Flavodoxin - cytochrome c553
  • Flavodoxin - cytochrome c
  • Nine-haem cytochrome c - cytochrome c3


Relevant publications:

  • Cunha, C.A., Romão, M.J., Sadeghi, S.J., Valetti, F., Gilardi, G., Soares, C.M. (1999) "Modelling of electron transfer complexes between flavodoxin and c-type cytochromes",JBIC, 4, 360-374
  • Sadeghi, S. J., Valetti, F., Cunha, C. A., Romão, M. J., Soares, C. M. and Gilardi, G. (2000) "Ionic strength dependence of the non-physiological electron transfer between flavodoxin and cytochrome c553 from D.vulgaris.", JBIC, 5, 730-737
  • Matias, P.M., Saraiva, L.M., Soares, C.M., Coelho, A.V., LeGall, J., Carrondo, M.A. (1999) "Nine-haem cytochrome c from Desulfovibrio desulfuricans ATCC 27774: primary sequence determination, crystallographic refinement at 1.8Å and modelling studies of its interaction with the tetrahaem cytochrome c3 from the same organism", JBIC, 4, 478-494
  • Carrondo, M.A., Soares, C.M., Matias, P.M. (2001) "Nine-haem cytochrome c", in Handbook of Metalloproteins
  • Matias, P.M., Soares, C.M., Saraiva, L.M., Coelho, R., Morais, J.,LeGall, J., Carrondo, M.A. (2001) "[NiFe] hydrogenase from Desulfovibrio desulfuricans ATCC 27774: primary sequence determination, crystallographicrefinement at 1.8 Å and modelling studies of its interaction withthe tetra-haem cytochrome c3", JBIC, 6, 63-81
  • Victor, B.L., Vicente, J.B., Rodrigues, R., Oliveira, S., Rodrigues-Pousada, C., Frazão, C., Gomes, C.M., Teixeira, M., Soares, C.M. (2003) "Docking and electron transfer studies between rubredoxin and rubredoxin:oxygen oxidoreductase", JBIC, 8, 475-488
  • Teixeira, V.H., Baptista, A.M., Soares, C.M. (2004) " Modelling electron transfer thermodynamics in protein complexes: interaction between two cytochromes c3, Biophys.J., 86, 2773-2785

 

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