Personal tools
You are here: Home / Labs / Animal Cell Technology Unit / Publications / Papers by topic (peer-review) / Cell and Process Engineering and Modeling

Cell and Process Engineering and Modeling

  1. Sa JV, Kleiderman S, Brito C, Sonnewald U, Leist M, Teixeira AP, Alves PM.  2017.  Structural and Functional insights into the catalytic mechanism of the Type II NADH: quinone oxidoreductase family.  NEUROCHEMICAL RESEARCH. 42, 244-253. http://dx.doi.org/10.1007/s11064-016-1907-z
  2. Teixeira AP and Fussenegger M. 2017. Synthetic biology-inspired therapies for metabolic diseases. Tissue, cell & pathway engineering. 47:59–66.  http://dx.doi.org/10.1016/j.copbio.2017.06.004
  3. Carinhas N, Koshkin A, Pais DAM, Alves PM and Teixeira AP. 2017. 13C-metabolic flux analysis of human adenovirus infection: Implications for viral vector production. Biotechnol. Bioeng., 114: 195–207. https://doi.org/10.1016/10.1002/bit.26063
  4. Abecasis B, Aguiar T, Arnault E, Costa R, Gomes-Alves P, Aspegren A, Serra M, Alves PM. 2017.  Expansion of 3D human induced pluripotent stem cell aggregates in bioreactors: Bioprocess intensification and scaling-up approaches.  J Biotechnol. 246:81-93. http://dx.doi.org/10.1016/j.jbiotec.2017.01.004
  5. Isidro IA, Portela RM, Clemente JJ, Cunha AE, Oliveira R.  2016.  Hybrid metabolic flux analysis and recombinant protein prediction in Pichia pastoris X-33 cultures expressing a single-chain antibody fragment.  Bioprocess Biosyst Eng.  39(9):1351-63. https://doi.org/10.1007/s00449-016-1611-z
  6. Carinhas N, Pais DAM, Koshkin A, Fernandes P, Coroadinha A, Carrondo MJT, Alves PM, Teixeira AP. 2016. Metabolic flux profilling of MDCK cells during growth and canine adenovirus vector production. Scientific Reports 6: 23529. http://www.nature.com/articles/srep23529
  7. Sousa MFQ, Silva MM, Roldão A, Alves PM, Serra M, Giroux D, Hashimura Y, Wesselschmidt R, Lee B, Carrondo MJT. 2015. Production of oncolytic adenovirus and human mesenchymal stem cells in a single-use, Vertical-Wheel bioreactor system: Impact of bioreactor design on performance of microcarrier-based cell culture processes. Biotechnol Progress. http://dx.doi.org/10.1002/btpr.2158.
  8. Silva AC, Simão D, Sousa MFQ, Küppers C, Lucas T, Cruz P, Carrondo MJ,  Kochanek S, Alves PM 2015 Human Amniocyte-derived Cells for the Production of Adenoviral Vectors. Biotechnology Journal, http://dx.doi.org/10.1002/biot.201400765
  9. Rodrigues AF, Carrondo MJ, Alves PM, Coroadinha AS 2015. Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cells. Trends in Biotechnology, 32(12):602-7. http://dx.doi.org/10.1016/j.tibtech.2014.09.010
  10. Duarte TM, Carinhas N, Barreiro LC, Carrondo MJT, Alves PM, Teixeira AP. 2014. Metabolic responses of CHO cells to limitation of key amino acids. Biotechnol Bioeng. Volume 111(10): 2095–2106. http://dx.doi.org/10.1002/bit.25266
  11. Rodrigues AF, Formas-Oliveira AS, Bandeira, VS, Alves PM, Hu WS, Coroadinha AS. 2013. Metabolic pathways recruited in the production of a recombinant enveloped virus: mining targets for process and cell engineering. Metab. Eng. 20: 131-137. http://dx.doi.org/10.1016/j.ymben.2013.10.001
  12. Monteiro F, Bernal V, Saelens X, Lozano AB, Bernal C , Sevilla A, Carrondo MJT, Alves PM. 2013 Metabolic profiling of insect cell lines: Unveiling cell line determinants behind system’s Productivity. Biotechnol Bioeng. 20: 131-145. http://dx.doi.org/10.1002/bit.25142
  13. Vidigal J, Dias MM, Fernandes F, Patrone M, Bispo C, Andrade C, Gardner R, Carrondo MJT, Alves PM, Teixeira AP 2013 A cell sorting protocol for selecting high-producing sub-populations of Sf9 and High FiveTM cells, J Biotech. 168 (4): 436-49.  http://dx.doi.org/10.1016/j.jbiotec.2013.10.020
  14. Carinhas N, Duarte TM, Barreiro LC, Carrondo MJT, Alves PM, Teixeira AP. 2013. Metabolic signatures of GS‑CHO cell clones associated with butyrate treatment and culture phase transition. Biotechnol Bioeng, 110 (12): 3244-57  http://dx.doi.org/10.1002/bit.24983
  15. Fernandes P, Peixoto C, Santiago VM, Kremer EJ, Coroadinha AS, Alves PM, 2013. Bioprocess development for canine adenovirus type 2 vectors. Gene Ther. Apr;20(4):353-60. http://dx.doi.org/10.1038/gt.2012.52
  16. Fernandes P, Santiago VM, Rodrigues AF, Tomás H, Kremer EJ, Alves PM, Coroadinha AS. 2013. Impact of E1 and Cre on Adenovirus Vector Amplification: Developing MDCK CAV-2-E1 and E1-Cre Transcomplementing Cell Lines. PLoS One. 2013;8(4):e60342. http://dx.doi.org/10.1371/journal.pone.0060342
  17. Fonseca CP, Fonseca LL, Montezinho LP, Alves PM, Santos H, Castro MM, Geraldes CF. 2013. (23)Na multiple quantum filtered NMR characterisation of Na(+) binding and dynamics in animal cells: a comparative study and effect of Na(+)/Li (+) competition. Eur Biophys J. Apr 6.  42(7): 503-519 http://dx.doi.org/10.1007/s00249-013-0899-8
  18. Carrondo MJ, Alves PM, Carinhas N, Glassey J, Hesse F, Merten OW, Micheletti M, Noll T, Oliveira R, Reichl U, Staby A, Teixeira AP, Weichert H, Mandenius CF, 2012. How can measurement, monitoring, modeling and control advance cell culture in industrial biotechnology? Biotechnol J, 7: 1522–1529. http://dx.doi.org/10.1002/biot.201200226
  19. Carinhas N, Oliveira R, Alves PM, Carrondo MJ, Teixeira AP, 2012. Systems biotechnology of animal cells: the road to prediction. Trends Biotechnol, 30(7):377-85 http://dx.doi.org/10.1016/j.tibtech.2012.03.004
  20. Almeida AS, Queiroga CS, Sousa MF, Alves PM, Vieira HL, 2012 Carbon monoxide modulates apoptosis by reinforcing oxidative metabolism in astrocytes: role of BCL-2. J Biol Chem, 287 (14): 10761-70. http://dx.doi.org/10.1074/jbc.M111.306738
  21. Queiroga CS, Tomasi S, Wideroe M, Alves PM, Vercelli A, Vieira HL, 2012. Preconditioning triggered by Carbon Monoxide (CO) provides neuronal protection following perinatal hypoxia-ischemia. Plos One, 7(8): e42632. http://dx.doi.org/10.1371/journal.pone.0042632
  22. Queiroga CS, Almeida AS and Vieira HL, 2012. Carbon monoxide targeting mitochondria. Biochem Res Int, 2012:749845. http://dx.doi.org/10.1155/2012/749845
  23. Tavares L, Figueira I, McDougall GJ, Vieira HL, Stewart D, Alves PM, Ferreira RB, Santos CN, 2012. Neuroprotective effects of digested polyphenols from wild blackberry species. Eur J Nutr. http://dx.doi.org/10.1007/s00394-012-0307-7
  24. Rodrigues AF, Amaral AI, Veríssimo V, Alves PM, Coroadinha AS, 2012. Adaptation of retrovirus producer cells to serum deprivation: Implications in lipid biosynthesis and vector production. Biotechnol Bioeng, 109 (5): 1269-79. http://dx.doi.org/10.1002/bit.24410
  25. Roldão A, Mellado MC, Lima JC, Carrondo MJ, Alves PM, Oliveira R, 2012. On the effect of thermodynamic equilibrium on the assembly efficiency of complex multi-layered virus-like particles (VLP): the case of rotavirus VLP. PLoS Comput Biol, 8(2): e1002367. http://dx.doi.org/10.1371/journal.pcbi.1002367
  26. Teixeira AP, Duarte TM, Carrondo MJ, Alves PM, 2011. Synchronous fluorescence spectroscopy as a novel tool to enable PAT applications in bioprocesses. Biotechnol Bioeng, 108(8):1852-61. http://dx.doi.org/10.1002/bit.23131
  27. Tostões RM, Leite SB, Miranda JP, Sousa MF, Wang DIC, Carrondo MJ, Alves PM, 2011. Perfusion of 3D encapsulated hepatocytes-a synergistic effect enhancing long-term functionality in bioreactors. Biotechnol Bioeng, 108: 41-49. http://dx.doi.org/10.1002/bit.22920
  28. Rodrigues AF, Guerreiro MR, Santiago VM, Dalba C, Klatzmann D, Alves PM, Carrondo MJ, Coroadinha AS, 2011. Down-regulation of CD81 tetraspanin in human cells producing retroviral-based particles: tailoring vector composition. Biotechnol Bioeng, 108(11):2623-33. http://dx.doi.org/10.1002/bit.23231
  29. Carinhas N, Robitaille AM, Moes S, Carrondo MJ, Jenoe P, Oliveira R, Alves PM, 2011. Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. Plos One, 6(10):e26444. http://dx.doi.org/10.1371/journal.pone.0026444
  30. Queiroga CS, Almeida AS, Alves PM, Brenner C, Vieira HL, 2011. Carbon monoxide prevents hepatic mitochondrial membrane permeabilization. BMC Cell Biol 12:10. http://dx.doi.org/10.1186/1471-2121-12-10
  31. Teixeira AP, Dias JM, Carinhas N, Sousa MF, Clemente JJ, Cunha AE, Von Stosch M, Alves PM, Carrondo MJ, Oliveira R, 2011. Cell functional enviromics: unravelling the function of environmental factors. BMC Syst Biol, 5:92. http://dx.doi.org/10.1186/1752-0509-5-92
  32. Carinhas N, Bernal V, Teixeira AP, Carrondo MJ, Alves PM, Oliveira R, 2011. Hybrid metabolic flux analysis: combining stoichiometric and statistical constraints to model the formation of complex recombinant products. BMC Syst Biol, 5:34. http://dx.doi.org/10.1186/1752-0509-5-34
  33. Teixeira AP, Duarte TM, Oliveira R, Carrondo MJ, Alves PM, 2011. High-throughput analysis of animal cell cultures using two dimensional fluorometry. J Biotechnol, 151:255-60. http://dx.doi.org/10.1016/j.jbiotec.2010.11.015
  34. Roldão A, Vicente T, Peixoto C, Carrondo MJ, Alves PM, 2011. Quality control and analytical methods for baculovirus based products. J Invertebr Pathol, 107:S94-S105. http://dx.doi.org/10.1016/j.jip.2011.05.009
  35. Carvalhal AV, Santos SS, Carrondo MJ, 2011. Extracellular purine and pyrimidine catabolism in cell culture. Biotechnol Prog, 27:1373-82. http://dx.doi.org/10.1002/btpr.656
  36. Carinhas N, Bernal V, Monteiro F, Carrondo MJ, Oliveira R, Alves PM, 2010. Improving baculovirus production at high cell density through manipulation of energy metabolism. Metabol Eng, 12:39-52. http://dx.doi.org/10.1016/j.ymben.2009.08.008
  37. Queiroga CS, Almeida AS, Martel C, Brenner C, Alves PM, Vieira HL, 2010. Glutathionylation of adenine nucleotide translocase induced by carbon monoxide prevents mitochondrial membrane permeabilisation and apoptosis. J Biol Chem, 285(22):17077-17088. http://dx.doi.org/10.1074/jbc.M109.065052
  38. Gama-Norton L, Herrmann S, Schucht R, Coroadinha AS, Löw R, Alves PM, Bartholomae CC, Schmidt M, Baum C, Schambach A, Hauser H, Wirth D, 2010. Retroviral vector performance in defined chromosomal Loci of modular packaging cell lines. Hum Gene Ther, 21(8):979-91. http://dx.doi.org/10.1089/hum.2009.089
  39. Teixeira AP, Oliveira R, Alves P, Carrondo MJ, 2009. Advances in on-line monitoring and control of mammalian cell cultures: supporting the PAT initiative. Biotechnol Adv, 27:726-32. http://dx.doi.org/10.1016/j.biotechadv.2009.05.003
  40. Carinhas N, Bernal V, Yokomizo AY, Carrondo MJ, Oliveira R, Alves PM, 2009. Baculovirus production for gene therapy: the role of cell density, multiplicity of infection and medium exchange. Appl Microbiol Biotechnol, 81(6):1041-49. http://dx.doi.org/10.1007/s00253-008-1727-4
  41. Roldão A, Oliveira R, Carrondo MJ, Alves PM, 2009. Error assessment in recombinant baculovirus titration: evaluation of different methods. J Virol Methods, 159:69-80. http://dx.doi.org/10.1016/j.jviromet.2009.03.007
  42. Teixeira AP, Portugal CA, Carinhas N, Dias JM, Crespo JP, Alves PM, Carrondo MJ, Oliveira R, 2009. In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures. Biotechnol Bioeng, 102(4):1098-106. http://dx.doi.org/10.1002/bit.22125
  43. Bernal V, Carinhas N, Yokomizo A, Carrondo MJ, Alves PM, 2009. Cell density effect in the Baculovirus-Insect cells system: a quantitative analysis of energetic metabolism. Biotechnol Bioeng, 104(1):162-80. http://dx.doi.org/10.1002/bit.22364
  44. Rodrigues AF, Carmo M, Alves PM, Coroadinha AS, 2009. Retroviral vector production under serum deprivation: the role of lipids. Biotechnol Bioeng, 104(6):1171-81. http://dx.doi.org/10.1002/bit.22499
  45. Teixeira AP, Santos SS, Carinhas N, Oliveira R, Alves PM, 2008. Combining metabolic flux analysis tools and 13C-NMR to estimate intracellular fluxes of cultured astrocytes. Neurochem Int, 52:478-86. http://dx.doi.org/10.1016/j.neuint.2007.08.007
  46. Roldão A, Carrondo MJ, Alves PM, Oliveira, R, 2008. Stochastic simulation of protein expression in the Baculovirus/Insect cell system. Comp Chem Eng, 32:68-77. http://dx.doi.org/10.1016/j.compchemeng.2007.04.017
  47. Vieira HL, Queiroga CS, Alves PM, 2008. Preconditioning induced by Carbon Monoxide provides neuronal protection against apoptosis. J Neurochem, 107:375-84. http://dx.doi.org/10.1111/j.1471-4159.2008.05610.x
  48. Clemente JJ, Monteiro SMS, Carrondo MJ, Cunha AE, 2008. Predicting sporulation events in a bioreactor using an Electronic Nose. Biotechnol Bioeng, 101(3):545-52. http://dx.doi.org/10.1002/bit.21920
  49. Teixeira A, Alves C, Alves PM, Carrondo MJ, Oliveira R, 2007. Hybrid elementary flux analysis/nonparametric modeling: application for bioprocess control. BMC Bioinformatics, 8(1):30. http://dx.doi.org/10.1186/1471-2105-8-30
  50. Roldão A, Vieira HL, Charpilienne A, Poncet D, Roy P, Carrondo MJ, Alves PM, Oliveira R, 2007. Modeling rotavirus-like particles production in a baculovirus expression vector system: infection kinetics, baculovirus DNA replication, mRNA synthesis and protein production. J Biotechnol, 128(4):875-94. http://dx.doi.org/10.1016/j.jbiotec.2007.01.003
  51. Teixeira AP, Carinhas N, Dias JM, Cruz PE, Alves PM, Carrondo MJ, Oliveira R, 2007. Hybrid semi-parametric mathematical systems: bridging the gap between biology and process engineering. J Biotechnol, 132:418-25. http://dx.doi.org/10.1016/j.jbiotec.2007.08.020
  52. Vieira HL, Pereira ACP, Carrondo MJ, Alves PM, 2006. Catalase effect on apoptosis for the improvement of recombinant protein production in baculovirus-insect cell system. Bioprocess Biosyst Eng, 29(5-6):409-14. http://dx.doi.org/10.1007/s00449-006-0093-9
  53. Roldão A, Vieira HL, Alves PM, Oliveira R, Carrondo MJ, 2006. Intracellular dynamics in Rotavirus-like particles production: Evaluation of multigene and monocistronic infection strategies. Proc Biochem, 41:2188-99 http://doi:10.1016/j.procbio.2006.06.019
  54. Teixeira AP, Clemente JJ, Cunha AE, Carrondo MJ, Oliveira R, 2006. Bioprocess iterative batch-to-batch optimization based on hybrid parametric/nonparametric models. Biotechnol Prog, 22(1):247-58. http://dx.doi.org/10.1021/bp0502328
  55. Teixeira A, Cunha AE, Clemente JJ, Moreira JL, Cruz HJ, Alves PM, Carrondo MJ, Oliveira R, 2005. Modelling and optimization of a recombinant BHK-21 cultivation process using hybrid grey-box systems. J Biotechnol, 118:290-303. http://dx.doi.org/10.1016/j.jbiotec.2005.04.024
  56. Oliveira R, Clemente JJ, Cunha AE, Carrondo MJ, 2005. Adaptive dissolved oxygen control through the glycerol feeding in a recombinant Pichia pastoris cultivation in conditions of oxygen transfer limitation. J Biotechnol, 116(1):35-50. http://dx.doi.org/10.1016/j.jbiotec.2004.09.016
  57. Monteiro SM, Clemente JJ, Henriques AO, Gomes RJ, Carrondo MJ, Cunha AE, 2005. A procedure for high-yield spore production by Bacillus subtilis. Biotechnol Prog, 21(4):1026-31. http://dx.doi.org/10.1021/bp050062z
  58. Kreij K, Mandenius CF, Clemente JJ, Cunha AE, Monteiro SM, Carrondo MJ, Hesse F, de Los Molinas MM, Wagner R, Merten OW, Gény-Fiamma C, Leger W, Wiesinger-Mayr H, Müller D, Katinger H, Mårtensson P, Bachinger T, Mitrovics J, 2005. On-line detection of microbial contaminations in animal cell reactor cultures using an electronic nose device. Cytotechnology, 48(1-3):41-58. http://dx.doi.org/10.1007/s10616-005-3587-4
  59. Maranga L, Cunha A, Clemente J, Cruz PE, Carrondo MJ, 2004. Scale-up of virus-like particles production: effects of sparging, agitation and bioreactor scale on cell growth, infection kinetics and productivity. J Biotechnol, 107:55-64. http://dx.doi.org/10.1016/j.jbiotec.2003.09.012
  60. Cunha AE, Clemente JJ, Gomes R, Pinto F, Thomaz M, Miranda S, Pinto R, Moosmayer D, Donner P, Carrondo MJ, 2004. Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol Bioeng, 86(4):458-67. http://dx.doi.org/10.1002/bit.20051
  61. Carvalhal AV, Santos SS, Calado J, Haury M, Carrondo MJ, 2003. Cell growth arrest by nucleotides, nucleosides and bases as a tool for improved production of recombinant proteins. Biotechnol Prog, 19:69-83. http://dx.doi.org/10.1021/bp0255917
  62. Carvalhal AV, Marcelino I, Carrondo MJ, 2003. Metabolic changes during cell growth inhibition by p27 overexpression. Appl Microbiol Biotechnol, 63:164-73. http://dx.doi.org/10.1007/s00253-003-1385-5
  63. Maranga L, AS Coroadinha, MJ Carrondo, 2002. Insect cell culture medium supplementation with fetal bovine serum and bovine serum albumin: effects on baculovirus adsorption and infection kinetics. Biotechnol Prog, 18:855-61. http://dx.doi.org/10.1021/bp025514b
  64. Carvalhal A, Coroadinha AS, Alves PM, Moreira JL, Hauser H, Carrondo MJ, 2002. Metabolic changes during cell growth inhibition by the IRF-1 system. Enzyme Microb Technol, 30:95-109. http://dx.doi.org/10.1016/S0141-0229(01)00460-4
  65. Cruz PE, L Maranga, MJ Carrondo, 2002. Integrated process optimization: lessons from retrovirus and virus-like particle production. J Biotechnol, 99:199-214. http://dx.doi.org/10.1016/S0168-1656(02)00210-9
  66. Fonseca LL, Alves PM, Carrondo MJ, Santos H, 2001. Effect of ethanol on the metabolism of primary astrocytes studied by [13C]- and [31P]-NMR spectroscopy. J Neurosci Res, 66:803-11. http://dx.doi.org/10.1002/jnr.10039
  67. Carvalhal AV, Moreira JL, Carrondo MJ, 2001. Strategies to modulate BHK cell proliferation by the regulation of IRF-1 expression. J Biotechnol, 92:47-59. http://dx.doi.org/10.1016/S0168-1656(01)00365-0
  68. Merten OW, Cruz PE, Rochette C, Geny-Fiamma C, Bouquet C, Goncalves D, Danos O, Carrondo MJ, 2001. Comparison of different bioreactor systems for the production of high titer retroviral vectors. Biotechnol Prog, 17:326-35. http://dx.doi.org/10.1021/bp000162z
  69. Cruz HJ, Dias EM, Peixoto CM, Moreira JL, Carrondo MJ, 2000. Product quality of a recombinant fusion protein expressed in immobilised baby hamster cells grown in protein-free medium. Biotechnol Lett, 22:677-82. http://dx.doi.org/10.1023/A:1005631602517
  70. Cruz PE, Almeida JS, Murphy PN, Moreira JL, Carrondo MJ, 2000. Modeling retrovirus production for gene therapy. 1. Determination of optimal bioreaction mode and harvest strategy. Biotechnol Prog, 16:213-21. http://dx.doi.org/10.1021/bp9901466
  71. Cruz PE, Goncalves D, Almeida J, Moreira JL, Carrondo MJ, 2000. Modeling retrovirus production for gene therapy. 2. Integrated optimization of bioreaction and downstream processing. Biotechnol Prog, 16:350-57. http://dx.doi.org/10.1021/bp000017l
  72. Cruz HJ, Peixoto CM, Nimtz M, Alves PM, Dias EM, Moreira JL, Carrondo MJ, 2000. Metabolic shifts do not influence the glycosylation patterns of a recombinant fusion protein expressed in BHK cells. Biotechnol Bioeng, 69:129-39. http://dx.doi.org/10.1002/(SICI)1097-0290(20000720)69:2<129::AID-BIT2>3.0.CO;2-5
  73. Cruz HJ, Freitas CM, Alves PM, Moreira JL, Carrondo MJ, 2000. Effects of ammonia and lactate on growth, metabolism, and productivity of BHK cells. Enzyme Microb Technol, 27:45-52. http://dx.doi.org/10.1016/S0141-0229(00)00151-4
  74. Cruz HJ, Moreira JL, Carrondo MJ, 2000. Metabolically optimised BHK cell fed-batch cultures. J Biotechnol, 80:109-18. http://dx.doi.org/10.1016/S0168-1656(00)00254-6
  75. Carvalhal AV, Moreira JL, Cruz H, Mueller P, Hauser H, Carrondo MJ, 2000. Manipulation of culture conditions for BHK cell growth inhibition by IRF-1 activation. Cytechnology, 32:135-45. http://dx.doi.org/10.1023/A:1008139304964
  76. Cruz HJ, JL Moreira, MJ Carrondo, 1999. Metabolic shifts by nutrient manipulation in continuous cultures of BHK cells. Biotechnol Bioeng, 66:104-13. http://dx.doi.org/10.1002/(SICI)1097-0290(1999)66:2%3C104::AID-BIT3%3E3.3.CO;2-R
  77. Cruz PE, Martins PC, Alves PM, Peixoto CC, Santos H, Moreira JL, Carrondo MJ,  1999. Proteolytic activity in infected and non infected insect cells: degradation of HIV-1 Pr55gag particles. Biotechnol Bioeng, 65:133-43. http://dx.doi.org/10.1002/(SICI)1097-0290(19991020)65:2%3C133::AID-BIT2%3E3.0.CO;2-X
  78. Cruz HJ, Ferreira AS, Freitas CM, Moreira JL, Carrondo MJ, 1999. Metabolic responses to different glucose and glutamine levels in baby hamster kidney cell culture. Appl Microbiol Biotechnol, 51: 579-85. http://dx.doi.org/10.1007/s002530051435
  79. Burger C, Carrondo MJ, Cruz H, Cuffe M, Dias E, Griffiths JB, Hayes K, Hauser H, Looby D, Mielke C, Moreira JL, Rieke E, Savage AV, Stacey GN, Welge T, 1999. An integrated strategy for the process development of a recombinant antibody-cytokine fusion protein expressed in BHK cells. Appl Microbiol Biotechnol, 52:345-53. http://dx.doi.org/10.1007/s002530051530
  80. Cruz HJ, Moreira JL, Stacey G, Dias EM, Hayes K, Looby D, Griffiths B, Carrondo MJ, 1998. Adaptation of BHK cells producing a recombinant protein to serum-free media and protein-free medium. Cytotechnology, 26:59-64. http://dx.doi.org/10.1023/A:1007951813755
  81. Cruz PE, Peixoto CC, Moreira JL, Carrondo MJ, 1998. Optimization of the production of virus-like particles in insect cells. Biotechnol Bioeng, 60:408-18. http://dx.doi.org/10.1002/(SICI)1097-0290(19981120)60:4<408::AID-BIT2>3.0.CO;2-Q
  82. Cruz PE, Peixoto CC, Moreira JL, Carrondo MJ, 1998. Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells. J Chem Technol Biotechnol, 72:149-58. http://dx.doi.org/10.1002/(SICI)1097-4660(199806)72:2%3C149::AID-JCTB886%3E3.3.CO;2-3
  83. Kirchhoff S, Kröger A, Cruz H, Tümmler M, Schaper F, Köster M, Hauser H, 1996. Regulation of cell growth by IRF-1 in BHK-21 cells. Cytotechnology, 22:147-56. http://dx.doi.org/10.1007/BF00353934
  84. Alves PM, JL Moreira, JM Rodrigues, JG Aunins, MJ Carrondo, 1996. Two-dimensional versus three-dimensional culture systems: effects on growth and productivity of BHK cells. Biotechnol Bioeng, 52:429-32. http://dx.doi.org/10.1002/(SICI)1097-0290(19961105)52:3<429::AID-BIT9>3.0.CO;2-M
  85. Moreira JL, Santana PC, Feliciano AS, Cruz PE, Racher AJ, Griffiths JB, Carrondo MJ, 1995. Effect of viscosity upon hydrodynamically controlled natural aggregates of animal cells grown in stirred vessels. Biotechnol Prog, 11:575-83. http://dx.doi.org/10.1021/bp00035a012
  86. Moreira JL, Cruz PE, Santana P, Aunins JG, Carrondo MJ, 1995. Formation and disruption of animal cell aggregates in stirred vessels: mechanisms and kinetic studies. Chem Eng Sci, 50:2747-64. http://dx.doi.org/10.1016/0009-2509(95)00118-O
  87. Xavier AL, Gonçalves J, Moreira JL, Carrondo MJ, 1995. Operational patterns affecting lactic acid production in ultrafiltration cell recycle bioreactor. Biotechnol Bioeng, 45:320-27. http://dx.doi.org/10.1002/bit.260450406
  88. Moreira JL, Alves PM, Aunins JG, Carrondo MJ, 1995. Hydrodynamic effects on BHK cells grown as suspended natural aggregates. Biotechnol Bioeng, 46:351-60. http://dx.doi.org/10.1002/bit.260460408
  89. Moreira JL, Alves PM, Feliciano A, Aunins JG, Carrondo MJ, 1995. Serum-free and serum-containing media for growth of suspended BHK aggregates in stirred vessels. Enzyme Microb Tech, 17:437-44. http://dx.doi.org/10.1016/0141-0229(94)00071-X
  90. Almeida JS, Reis MA, Carrondo MJ, 1995. Competition between nitrate and nitrite reduction in denitrification by Pseudomonas fluorescens. Biotechnol Bioeng, 46(5):476-84. http://dx.doi.org/10.1002/bit.260460512
  91. Almeida JS, Júlio SM, Reis MA, Carrondo MJ, 1995. Nitrite inhibition of denitrification by Pseudomonas fluorescens. Biotechnol Bioeng, 46(3):194-201. http://dx.doi.org/10.1002/bit.260460303
  92. Racher AJ, Moreira JL, Alves PM, Wirth M, Weidle UH, Hauser H, Carrondo MJ, Griffiths JB, 1994. Expression of recombinant antibody and secreted alkaline phosphatase in mammalian cells. Influence of cell line and culture system upon production kinetics. Appl Microbiol Biotechnol, 40:851-6. http://dx.doi.org/10.1007/BF00173987
  93. Moreira JL, Alves PM, Aunins JG, Carrondo MJ, 1994. Changes in animal cell natural aggregates in suspended batch cultures. Appl Microbiol Biotechnol, 41: 203-9. http://dx.doi.org/10.1007/BF00186960
  94. Moreira JL, Feliciano AS, Santana PC, Cruz PE, Aunins JG, Carrondo MJ, 1994. Repeated-batch cultures of baby hamster kidney cell aggregates in stirred vessels. Cytotechnology, 15:337-49. http://dx.doi.org/10.1007/BF00762409
  95. Moreira JL, Alves PM, Rodrigues JM, Cruz PE, Aunins JG, Carrondo MJ, 1994. Studies of baby hamster kidney natural cell aggregation in suspended batch cultures. Ann N Y Acad Sci, 745:122-33. http://dx.doi.org/10.1111/j.1749-6632.1994.tb44368.x
  96. Moreira JL, Wirth M, Fitzek M, Hauser H, 1992. Evaluation of reporter genes in mammalian cell lines. Meth Mol Cell Biol, 3:23-2.
  97. Reis MA, Almeida JS, Lemos PC, Carrondo MJ, 1992. Effect of hydrogen sulfide on growth of sulfate reducing bacteria. Biotechnol Bioeng, 40(5):593-600. http://dx.doi.org/10.1002/bit.260400506
  98. Crespo JP, Almeida JS, Moura MJ, Carrondo MJ, 1990. Modeling of immobilized cell reactor for propionic acid fermentation. Biotechnol Bioeng, 36(7):705-16. http://dx.doi.org/10.1002/bit.260360708
  99. Carrondo MJ, Reboredo F, Ganho RM, Oliveira JF, 1984. Analysis of sediments for heavy metals by a rapid electrothermal atomic-absorption procedure. Talanta, 31(7):561-4. http://dx.doi.org/10.1016/0039-9140(84)80141-1
  100. Carrondo MJ, Lester JN, Perry R, 1979. An investigation of a flameless atomic-absorption method for determination of aluminium, calcium, iron and magnesium in sewage sludge. Talanta, 26(10):929-33. http://dx.doi.org/10.1016/0039-9140(79)80128-9
Document Actions