Personal tools
You are here: Home / Labs / Animal Cell Technology Unit / Publications / Papers by topic (peer-review) / Downstream Processing

Downstream Processing

  1. Cunha B, Aguiar T, Carvalho SB, Silva MM, Gomes RA, Carrondo MJT, Gomes-Alves P, Peixoto C, Serra M, Alves PM. 2017. Bioprocess integration for human mesenchymal stem cells: from up to downstream processing scale-up to cell proteome characterization.  J Biotechnol. S0168-1656(17)30031-7.  https://doi.org/10.1016/j.jbiotec.2017.01.014
  2. Carvalho SB, Freire JM, Moleirinho MG, Monteiro F, Gaspar D, Castanho MA, Carrondo MJ, Alves PM, Bernardes GJ, Peixoto C. 2016. Bioorthogonal Strategy for Bioprocessing of Specific-Site-Functionalized Enveloped Influenza-Virus-Like Particles. Bioconjugate Chemistry. 2386–2399. https://doi.org/10.1021/acs.bioconjchem.6b00372
  3. Cunha B, Silva RJ, Aguiar T, Serra M, Daicic J, Maloisel JL, Clachan J, Åkerblom A, Carrondo MJ, Peixoto C, Alves PM. 2016. Improving washing strategies of human mesenchymal stem cells using negative mode expanded bed chromatography. J Chromatogr A. 2016 Jan 15;1429:292-303. http://dx.doi.org/10.1016/j.chroma.2015.12.052
  4. Silva R, Mota JP, Peixoto C, Alves PM, Carrondo MJT. 2015. Improving the downstream processing of vaccine and gene therapy vectors with continuous chromatography. Pharmaceutical Bioprocessing 3(8): 489-505. http://dx.doi.org/10.4155/pbp.15.29
  5. Nestola P, Peixoto C, Villain L, Alves PM, Carrondo MJT, Mota JPB. 2015. Rational development of two flowthrough purification strategies for adenovirus type 5 and retrovirus-like particles, Journal of Chromatography A. http://dx.doi.org/10.1016/j.chroma.2015.11.037
  6. Cunha B, Aguiar T, Silva MM, Silva R, Sousa MF, Pineda E, Peixoto C, Carrondo MJ, Serra M, Alves PM. 2015. Exploring continuous and integrated strategies for the up- and downstream processing of human mesenchymal stem cells. Journal of Biotechnology - special issue on integrated continuous biomanufacturing, ahead of print 2015 Mar 4, http://dx.doi.org/10.1016/j.jbiotec.2015.02.023  
  7. Cunha B, Peixoto C, Silva M, Carrondo MJT, Serra M, Alves PM 2015. Filtration methodologies for the clarification and concentration of human mesenchymal stem cells. Journal of Membrane Science, 478:117-129. http://dx.doi.org/10.1016/j.memsci.2014.12.041
  8. Nestola P, Peixoto C, Silva RJS, Alves PM, Mota JPB, Carrondo MJT 2015. Improved virus purification processes for vaccines and gene therapy. Biotechnology and Bioengineering, 112(5):843-57. http://dx.doi.org/10.1002/bit.25545
  9. Nestola P, Silva RJS, Peixoto C, Alves PM, Carrondo MJT, Mota JPB 2015. Robust design of adenovirus purification by two-column, simulated countercurrent, size-exclusion chromatography. Journal of Biotechnology; in press. http://dx.doi.org/10.1016/j.jbiotec.2015.01.030
  10. Nestola P, Martins DL, Peixoto C, Roederstein S, Schleuss T, Alves PM, Mota JPB, Carrondo MJ. 2014. Evaluation of Novel Large Cut-Off Ultrafiltration Membranes for Adenovirus Serotype 5 (Ad5) Concentration. PLoS ONE, 9(12):-115802. http://dx.doi.org/10.1371/journal.pone.0115802
  11. Vlaming M, van Duijn E, Dillingh MR, Brands R, Windhorst AD, Hendrikse NH, Bosgra S, Burggraaf J, de Koning MC, Fidder A, Mocking J, Sandman H, de Ligt R, Fabriek BO, Pasman WJ, Seinen W, Alves T, Carrondo M, Peixoto C, Peeters P, Vaes W. 2015. Microdosing of a carbon-14 labeled protein in healthy volunteers accurately predicts its pharmacokinetics at therapeutic dosages. Clin Pharmacol Ther. Apr13. http://dx.doi.org/10.1002/cpt.131
  12. Gonçalves CSM, Sousa M, Martins DL, Barroso T, Pina AS, Peixoto C, Ricardo A, Roque CA. 2015. Biobased Monoliths for Adenovirus Purification. ACS Appl. Mater. Interfaces, 7 (12), pp 6605–6612. http://dx.doi.org/10.1021/am508907b
  13. Nestola P, Silva RJS, Peixoto C, Alves PM, Carrondo MJT, Mota JPB. 2014. Adenovirus purification by two-column, size-exclusion, simulated countercurrent chromatography. Journal of Chromatography A,1347: 111–121. http://dx.doi.org/10.1016/j.chroma.2014.04.079  
  14. Nestola P, Villain L, Peixoto C, Martins DL, Alves PM, Carrondo MJT, Mota JPB. 2014. Impact of grafting on the design of new membrane adsorbers for adenovirus purification. Journal of Biotechnology, 181: 1–11. http://dx.doi.org/10.1016/j.jbiotec.2014.04.003
  15. Fernandes P, Peixoto C, Santiago VM, Kremer EJ, Coroadinha AS, Alves PM, 2013. Bioprocess development for canine adenovirus type 2 vectors. Gene Ther. Apr;20(4):353-60. http://dx.doi.org/10.1038/gt.2012.52
  16. Bandeira V, Peixoto C, Rodrigues AF, Cruz PE, Alves PM, Coroadinha AS, Carrondo MJ, 2012. Downstream processing of lentiviral vectors: releasing bottlenecks. Hum Gene Ther Methods, 23(4):255-63. http://dx.doi.org/10.1089/hgtb.2012.059
  17. Vicente T, Mota JP, Peixoto C, Alves PM, Carrondo MJ, 2011. Rational design and optimization of downstream processes of virus particles for biopharmaceutical applications: current advances. Biotechnol Adv, 29: 869-78. http://dx.doi.org/10.1016/j.biotechadv.2011.07.004
  18. Lesch HP, Laitinen A, Peixoto C, Vicente T, Makkonen ME, Laitinen L, Pikkarainen JT, Samaranayake H, Alves PM, Carrondo MJ, Ylä-Herttuala S, Airenne KJ, 2011. Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors. Gene Ther, 18:531-38. http://dx.doi.org/10.1038/gt.2010.162
  19. Vicente T, Roldão A, Peixoto C, Carrondo MJ, Alves PM, 2011. Large-scale production and purification of VLP-based vaccines. J Invertebr Pathol, 107:S42-S48. http://dx.doi.org/10.1016/j.jip.2011.05.004
  20. Vicente T, Fáber R, Alves PM, Carrondo MJ, Mota JP, 2011. Impact of ligand density on the optimization of ion-exchange membrane chromatography for viral vector purification. Biotechnol Bioeng, 108(6):1347-59. http://dx.doi.org/10.1002/bit.23058
  21. Roldão A, Vicente T, Peixoto C, Carrondo MJ, Alves PM, 2011. Quality control and analytical methods for baculovirus based products. J Invertebr Pathol, 107:S94-S105. http://dx.doi.org/10.1016/j.jip.2011.05.009
  22. Vicente T, Mota JP, Peixoto C, Alves PM, Carrondo MJ, 2010. Analysis of adsorption of a baculovirus bioreaction bulk on an ion-exchange surface by surface plasmon resonance. J Biotechnol, 148(4):171-81. http://dx.doi.org/10.1016/j.jbiotec.2010.05.005
  23. Vicente T, Mota JP, Peixoto C, Alves PM, Carrondo MJ, 2010. Modeling protein binding and elution over a chromatographic surface probed by surface plasmon resonance. J Chromatogr A, 1217:2032-41. http://dx.doi.org/10.1016/j.chroma.2010.01.068
  24. Vicente T, Peixoto C, Alves PM, Carrondo MJ, 2010. Modeling electrostatic interactions of baculovirus vectors for ion-exchange process development. J Chromatogr A, 1217:3754-64. http://dx.doi.org/10.1016/j.chroma.2010.03.059
  25. Vicente T, Peixoto C, Carrondo MJ, Alves PM, 2009. Purification of recombinant baculoviruses for gene therapy using membrane processes. Gene Ther, 16:766-775. http://dx.doi.org/10.1038/gt.2009.33
  26. Mellado MC, Peixoto C, Cruz PE, Carrondo MJ, Alves PM, 2008. Purification of recombinant rotavirus VP7 for the study of in vitro rotavirus-like particles assembly. J Chromatogr B, 874:89-94. http://dx.doi.org/10.1016/j.jchromb.2008.09.002
  27. Peixoto C, Ferreira TB, Sousa MF, Carrondo MJ, Alves PM, 2008. Towards purification of adenovirus vectors based on membrane technology. Biotechnol Prog, 24:1290-96. http://dx.doi.org/10.1002/btpr.25
  28. Rodrigues T, Alves A, Lopes A, Carrondo MJ, Alves PM, Cruz PE, 2008. Removal of envelope protein-free retroviral vectors by anion exchange chromatography to improve product quality. J Sep Sci, 31:3509-18. http://dx.doi.org/10.1002/jssc.200800195
  29. Mellado MC, Franco C, Coelho AV, AlvesPM, Simplício AL, 2008. Sodium dodecyl sulfate-capillary gel electrophoresis analysis of rotavirus-like particles. J Chromatogr A, 1192:166-72. http://dx.doi.org/10.1016/j.chroma.2008.03.035
  30. Vicente T, Sousa MF, Peixoto C, Mota JP, Alves PM, Carrondo MJ, 2008. Anion exchange membrane chromatography for purification of rotavirus-like particles. J Membr Sci, 311:270-83. http://dx.doi.org/10.1016/j.memsci.2007.12.021
  31. Peixoto C, Marcelino I, Amaral AI, Carrondo MJ, Alves PM, 2007. Purification by membrane technology of an intracellular Ehrlichia ruminantium candidate vaccine against heartwater. Proc Biochem, 42(7):1084-89. http://dx.doi.org/10.1016/j.procbio.2007.04.012
  32. Rodrigues T, Carvalho A, Carmo M, Carrondo MJ, Alves PM, Cruz PE, 2007. Scaleable purification process for gene therapy retroviral vectors. J Gene Med, 9(4):233-43. http://dx.doi.org/10.1002/jgm.1021
  33. Peixoto C, Sousa MF, Silva AC, Carrondo MJ, Alves PM, 2007. Downstream processing of triple-layered rotavirus like particles. J Biotechnol, 127:452-61 http://dx.doi.org/10.1016/j.jbiotec.2006.08.002
  34. Rodrigues T, Carrondo MJ, Alves PM, Cruz PE, 2007. Purification of retroviral vectors for clinical application: biological implication and technological challenges. J Biotechnol, 127:520-41. http://dx.doi.org/10.1016/j.jbiotec.2006.07.028
  35. Rodrigues T, Carvalho A, Roldao A, Carrondo MJ, Alves PM, Cruz PE, 2006. Screening anion-exchange chromatographic matrices for isolation of onco-retroviral vectors. J Chromatogr B, 837(1-2):59-68. http://dx.doi.org/10.1016/j.jchromb.2006.03.061
  36. Peixoto C, Ferreira TB, Carrondo MJ, Cruz PE, Alves PM, 2006. Purification of adenoviral vectors using expanded bed chromatography. J Virol Methods, 132:121-6. http://dx.doi.org/10.1016/j.jviromet.2005.10.002
  37. Peixoto CC, Marcelino I, Vachiéry N, Bensaid A, Martinez D, Carrondo MJ, Alves PM, 2005. Quantification of Ehrlichia ruminantium by real time PCR. Vet Microbiol, 107:273-8. http://dx.doi.org/10.1016/j.vetmic.2005.02.001
  38. Souza AP, Peixoto CC, Maranga L, Carvalhal AV, Moraes RH, Mendonça RZ, Pereira CA, Carrondo MJ, Mendonça RZ, 2005. Purification and characterization of an anti-apoptotic protein isolated from Lonomia obliqua Hemolymph. Biotechnol Prog, 21:99-105. http://dx.doi.org/10.1021/bp049831p
  39. Carmo M,  Peixoto CC, Coroadinha AS, Alves PM, Cruz PE, Carrondo MJ, 2004. Quantitation of MLV-based retroviral vectors using real-time RT-PCR. J Virol Methods, 119:115-9. http://dx.doi.org/10.1016/j.jviromet.2004.03.007
  40. Maranga L, Rueda P, Antonis AF, Vela C, Langeveld JP, Casal JI, Carrondo MJ, 2002. Large scale production and downstream processing of a recombinant porcine parvovirus vaccine. Appl Microbiol Biotechnol, 59:45-50. http://dx.doi.org/10.1007/s00253-002-0976-x
  41. Cruz PE, Peixoto CC, Devos K, Moreira JL, Saman E, Carrondo MJ, 2000. Characterization and downstream processing of HIV-1 core and virus-like-particles produced in serum free medium. Enzyme Microb Technol, 26:61-70. http://dx.doi.org/10.1016/S0141-0229(99)00128-3

 

Document Actions