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Gene Therapy

  1. Bandeira VS, Tomas HÁ, Alice E, Carrondo MJT, Coroadinha AS. 2017. Disclosing the Parameters Leading to High Productivity of Retroviral Producer Cells Lines: Evaluating Random Versus Targeted Integration. HUMAN GENE THERAPY METHODS. 28, 78-90.  http://dx.doi.org/10.1089/hgtb.2016.149
  2. Soares HR, Castro R, Tomas HA, Rodrigues AF, Gomes-Alves P, Bellier B, Klatzmann D, Carrondo MJ, Alves PM, Coroadinha AS. 2016. Tetraspanins displayed in retrovirus-derived virus-like particles and their immunogenicity. Vaccine. http://dx.doi.org/10.1016/j.vaccine.2015.12.015
  3. Fernandes CS, Castro R, Coroadinha AS, Roque AC. 2016. Small synthetic ligands for the enrichment of viral particles pseudotyped with amphotropic murine leukemia virus envelope. J Chromatogr A. pii: S0021-9673(16)30125-X. http://dx.doi.org/10.1016/j.chroma.2016.02.026
  4. Silva AC, Teixeira AP, Alves PM. 2016. Impact of Adenovirus infection in host cell metabolism evaluated by 1H NMR spectroscopy. J Biotechnol. http://dx.doi.org/10.1016/j.jbiotec.2016.05.025
  5. Fernandes CS, Castro R, Coroadinha AS, Roque AC. 2016. Small synthetic ligands for the enrichment of viral particles pseudotyped with amphotropic murine leukemia virus envelope. J Chromatogr A. pii: S0021-9673(16)30125-X. http://dx.doi.org/10.1016/j.chroma.2016.02.026
  6. Simão D, Pinto C, Fernandes P, Peddie CJ, Piersanti S, Collinson LM, Salinas S, Saggio I, Schiavo G, Kremer EJ, Brito C, Alves PM. 2016. Evaluation of helper-dependent canine adenovirus vectors in a 3D human CNS model. Gene Ther. http://dx.doi.org/10.1038/gt.2015.75
  7. Rodrigues A F, Guerreiro M R, Formas-Oliveira A S, Fernandes P, Blechert A-K, Genzel Y, Alves P M, Hu W S, Coroadinha AS. 2015. Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK. Biotechnology and Bioengineering Jul 1. http://dx.doi.org/10.1002/bit.25691.
  8. Rodrigues AF, Soares HR, Guerreiro MR, Alves PM, Coroadinha AS. 2015. Viral vaccines and their manufacturing cell substrates: New trends and designs in modern vaccinology. Biotechnol J. Sep;10(9):1329-44. http://dx.doi.org/10.1002/biot.201400387.
  9. Rodrigues AF, Formas-Oliveira AS, Guerreiro M, Tomas H, Alves PM and Coroadinha AS. 2015. Single step cloning-screening method: a new tool for developing and studying high titer viral vector producer cells. Gene Ther, May 4. http://dx.doi.org/10.1038/gt.2015.44
  10. Castro R, Fernandes P, Laske T, Sousa MFQ, Genzel Y, Scharfenberg K, Alves PM, Coroadinha AS. 2015. Production of canine adenovirus type 2 in serum-free suspension cultures of MDCK cells. Appl Microbiol Biotechnol http://dx.doi.org/10.1007/s00253-015-6636-8
  11. Nestola P, Peixoto C, Silva RJS, Alves PM, Mota JPB, Carrondo MJT 2015. Improved virus purification processes for vaccines and gene therapy. Biotechnology and Bioengineering, 112(5):843-57. http://dx.doi.org/10.1002/bit.25545
  12. Fernandes P, Simão D, Guerreiro MR, Kremer EJ, Coroadinha AS, Alves PM. 2015. Impact of adenovirus life cycle progression on the generation of canine helper-dependent vectors. Gene Ther. Jan;22(1):40-9. http://dx.doi.org/10.1038/gt.2014.92
  13. Fernandes P, Almeida AI, Kremer EJ, Alves PM, Coroadinha AS. 2015. Canine helper-dependent vectors production: implications of Cre activity and co-infection on adenovirus propagation. Sci Rep. Mar 16;5:9135. http://dx.doi.org/10.1038/srep09135
  14. Nestola P, Silva RJS, Peixoto C, Alves PM, Carrondo MJT, Mota JPB 2015. Robust design of adenovirus purification by two-column, simulated countercurrent, size-exclusion chromatography. Journal of Biotechnology; http://dx.doi.org/10.1016/j.jbiotec.2015.01.030
  15. Fernandes P, Simão D, Guerreiro MR, Kremer EJ, Coroadinha AS, Alves PM. 2015. Impact of adenovirus life cycle on the generation of canine helper-dependent vectors. Gen Ther 22, 40-49. http://dx.doi.org/10.1038/gt.2014.92  
  16. Rodrigues AF, Carrondo MJ, Alves PM, Coroadinha AS, 2014. Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cells. Trends in Biotechnology Dec;32(12):602-7. http://dx.doi.org/10.1016/j.tibtech.2014.09.010
  17. Nestola P, Villain L, Peixoto C, Martins DL, Alves PM, Carrondo MJT, Mota JPB, 2014.
  18. Impact of grafting on the design of new membrane adsorbers for adenovirus purification.
  19. Journal of Biotechnology, 181: 1–11. http://dx.doi.org/10.1016/j.jbiotec.2014.04.003
  20. Nestola P, Silva RJS, Peixoto C, Alves PM, Carrondo MJT, Mota JPB. 2014. Adenovirus purification by two-column, size-exclusion, simulated countercurrent chromatography. Journal of Chromatography A,1347: 111–121. http://dx.doi.org/10.1016/j.chroma.2014.04.079  
  21. Rodrigues AF, Formas-Oliveira AS, Bandeira, VS, Alves PM, Hu WS, Coroadinha AS. 2013. Metabolic pathways recruited in the production of a recombinant enveloped virus: mining targets for process and cell engineering. Metab. Eng., 20, 131-137. http://dx.doi.org/10.1016/j.ymben.2013.10.001
  22. Fernandes P, Peixoto C, Santiago VM, Kremer EJ, Coroadinha AS, Alves PM, 2013. Bioprocess development for canine adenovirus type 2 vectors. Gene Ther. Apr;20(4):353-60. http://dx.doi.org/10.1038/gt.2012.52
  23. Fernandes P, Santiago VM, Rodrigues AF, Tomás H, Kremer EJ, Alves PM, Coroadinha AS. 2013. Impact of E1 and Cre on Adenovirus Vector Amplification: Developing MDCK CAV-2-E1 and E1-Cre Transcomplementing Cell Lines. PLoS One.8(4):e60342. http://dx.doi.org/10.1371/journal.pone.0060342
  24. Rodrigues AF, Amaral AI, Veríssimo V, Alves PM, Coroadinha AS, 2012. Adaptation of retrovirus producer cells to serum deprivation: Implications in lipid biosynthesis and vector production. Biotechnol Bioeng, 109 (5): 1269-79. http://dx.doi.org/10.1002/bit.24410
  25. Carrondo MJ, Panet A, Wirth D, Coroadinha AS, Cruz P, Falk H, Schucht R, Dupont F, Geny-Fiamma C, Merten OW, Hauser H, 2011. Integrated strategy for the production of therapeutic retroviral vectors. Hum Gene Ther, 22(3): 370-79. http://dx.doi.org/10.1089/hum.2009.165
  26. Gama-Norton L, Botezatu L, Herrmann S, Schweizer M, Alves PM, Hauser H, Wirth D, 2011. Lentivirus production is influenced by SV40 large T-antigen and chromosomal integration of the vector in HEK293 cells. Hum Gene Ther, 22(10):1269-79. http://dx.doi.org/10.1089/hum.2010.143
  27. Real G, Monteiro F, Burger C, Alves PM, 2011. Improvement of lentiviral transfer vectors using cis-acting regulatory elements for increased gene expression. Appl Microbiol Biotechnol, 91:1581-91. http://dx.doi.org/10.1007/s00253-011-3392-2
  28. Cruz PE, Rodrigues T, Carmo M, Wirth D, Amaral AI, Alves PM, Coroadinha AS, 2011. Manufacturing of retroviruses. Methods Mol Biol, 737:157-82. http://dx.doi.org/10.1007/978-1-61779-095-9_7
  29. Silva AC, Peixoto C, Lucas T, Kuppers C, Cruz PE, Alves PM, Kochanek S, 2010. Adenovirus vector production and purification. Curr Gene Ther, 10(6):437-55.
  30. Coroadinha AS, Gama-Norton L, Amaral AI, Hauser H, Alves PM, Cruz PE, 2010. Production of retroviral vectors. Curr Gene Ther, 10(6):456-73.
  31. Bernal V, Carinhas N, Yokomizo A, Carrondo MJ, Alves PM, 2009. Cell density effect in the Baculovirus-Insect cells system: a quantitative analysis of energetic metabolism. Biotechnol Bioeng, 104(1):162-80. http://dx.doi.org/10.1002/bit.22364
  32. Carinhas N, Bernal V, Yokomizo AY, Carrondo MJ, Oliveira R, Alves PM, 2009. Baculovirus production for gene therapy: the role of cell density, multiplicity of infection and medium exchange. Appl Microbiol Biotechnol, 81(6):1041-49. http://dx.doi.org/10.1007/s00253-008-1727-4
  33. Rodrigues AF, Carmo M, Alves PM, Coroadinha AS, 2009. Retroviral vector production under serum deprivation: the role of lipids. Biotechnol Bioeng, 104(6):1171-81. http://dx.doi.org/10.1002/bit.22499
  34. Vicente T, Peixoto C, Carrondo MJ, Alves PM, 2009. Virus production for clinical gene therapy. Methods Mol Biol, 542:447-70. http://dx.doi.org/10.1007/978-1-59745-561-9_24
  35. Vicente T, Peixoto C, Carrondo MJ, Alves PM, 2009. Purification of recombinant baculoviruses for gene therapy using membrane processes. Gene Ther, 16:766-775. http://dx.doi.org/10.1038/gt.2009.33
  36. Carmo M, Alves A, Rodrigues AF, Coroadinha AS, Carrondo MJ, Alves PM, Cruz PE, 2009 Stabilization of gammaretroviral and lentiviral vectors: from production to gene transfer. J Gene Med, 11:670-78. http://dx.doi.org/10.1002/jgm.1353
  37. Ferreira TB, Perdigão R, Silva AC, Zhang C, Aunins JG, Carrondo MJ, Alves PM, 2009. 293 cell cycle synchronisation in adenovirus vector production. Biotechnol Prog, 25(1):235-43. http://dx.doi.org/10.1002/btpr.64
  38. Carmo M, Dias JD, Panet A, Coroadinha AS, Carrondo MJ, Alves PM, Cruz PE, 2009. Thermosensitivity of the reverse transcription process as an inactivation mechanism of lentiviral vectors. Hum Gene Ther, 20(10):1168-76. http://dx.doi.org/10.1089/hum.2009.068
  39. Amaral AI, Coroadinha AS, Merten O-W, Alves PM, 2008. Improving retroviral vectors production: role of carbon sources in lipid biosynthesis. J Biotechnol, 138: 57-66. http://dx.doi.org/10.1016/j.jbiotec.2008.08.005
  40. Carmo M, Panet A, Carrondo MJ, Alves PM, Cruz PE, 2008. From retroviral vector production to gene transfer: spontaneous inactivation is caused by loss of reverse transcription capacity. J Gene Med, 10:383-91. http://dx.doi.org/10.1002/jgm.1163
  41. Rodrigues T, Alves A, Lopes A, Carrondo MJ, Alves PM, Cruz PE, 2008. Removal of envelope protein-free retroviral vectors by anion exchange chromatography to improve product quality. J Sep Sci, 31:3509-18. http://dx.doi.org/10.1002/jssc.200800195
  42. Carrondo MJ, Merten OW, Haury M, Alves PM, Coroadinha AS, 2008. Impact of retroviral vector components stoichiometry on packaging cell lines: effects on productivity and vector quality. Hum Gene Ther, 19:199-210. http://dx.doi.org/doi:10.1089/hum.2007.0101
  43. Ferreira TB, Carrondo MJ, Alves PM, 2007. Effect of ammonia production on intracellular pH consequent effect on adenovirus vector production. J Biotechnol, 129(3):433-38. http://dx.doi.org/10.1016/j.jbiotec.2007.01.010
  44. Rodrigues T, Carvalho A, Carmo M, Carrondo MJ, Alves PM, Cruz PE, 2007. Scaleable purification process for gene therapy retroviral vectors. J Gene Med, 9(4):233-43. http://dx.doi.org/10.1002/jgm.1021
  45. Rodrigues T, Carrondo MJ, Alves PM, Cruz PE, 2007. Purification of retroviral vectors for clinical application: biological implication and technological challenges. J Biotechnol, 127:520-41. http://dx.doi.org/10.1016/j.jbiotec.2006.07.028
  46. Wirth D, Gama-Norton L, Riemer P, Sandhu U, Schucht R, Hauser H, 2007. Road to precision: recombinase-based targeting technologies for genome engineering. Curr Opin Biotechnol, 18:411–19. http://dx.doi.org/10.1016/j.copbio.2007.07.013
  47. Coroadinha AS, Silva AC, Pires E, Coelho A, Alves PM, Carrondo MJ, 2006. Effect of osmotic pressure on the production of retroviral vectors: enhancement in vector stability. Biotechnol Bioeng, 94(2):322-9. http://dx.doi.org/10.1002/bit.20847
  48. Rodrigues T, Carvalho A, Roldao A, Carrondo MJ, Alves PM, Cruz PE, 2006. Screening anion-exchange chromatographic matrices for isolation of onco-retroviral vectors. J Chromatogr B, 837(1-2):59-68. http://dx.doi.org/10.1016/j.jchromb.2006.03.061
  49. Schucht R, Coroadinha AS, Zanta-Boussif MA, Verhoeyen E, Carrondo MJ, Hauser H, Wirth D, 2006. A new generation of retroviral producer cells: predictable and stable virus production by Flp-mediated site-specific integration of retroviral vectors. Mol Ther, 14(2):285-92. http://dx.doi.org/10.1016/j.ymthe.2005.12.003
  50. Carmo M, Faria TQ, Falk H, Coroadinha AS, Teixeira M, Merten OW, Geny-Fiamma C, Alves PM, Danos O, Panet A, Carrondo MJ, Cruz PE, 2006. Relationship between retroviral vector membrane and vector stability. J Gen Virol, 87(5):1349-56. http://dx.doi.org/10.1099/vir.0.81302-0
  51. Cruz PE, Silva AC, Roldao A, Carmo M, Carrondo MJ, Alves PM, 2006.Screening of novel excipients for improving the stability of retroviral and adenoviral vectors. Biotechnol Prog, 22(2):568-76. http://dx.doi.org/10.1021/bp050294y
  52. Coroadinha AS, Ribeiro J, Roldão A, Cruz PE, Alves PM, Merten O-W, Carrondo MJ, 2006. Effect of medium sugar source on the production of retroviral vectors for gene therapy. Biotechnol Bioeng, 94(1):24-36. http://dx.doi.org/10.1002/bit.20778
  53. Coroadinha AS, Alves PM, Santos SS, Cruz PE, Merten, OW, Carrondo MJ, 2006. Retrovirus producer cell line metabolism: implications on viral productivity. Appl Microbiol Biotechnol. 72(6):1125-35. http://dx.doi.org/10.1007/s00253-006-0401-y
  54. Coroadinha AS, Schucht R, Gama-Norton L, Wirth D, Hauser H, Carrondo MJ, 2006. The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: predictability and efficiency by transgene exchange. J Biotechnol, 124(2):457-68. http://dx.doi.org/10.1016/j.jbiotec.2006.01.037
  55. Ferreira TB, Alves PM, Aunins JG, Carrondo MJ, 2005. Use of adenoviral vectors as veterinary vaccines. Gene Ther, 12(S1):73-83. http://dx.doi.org/10.1038/sj.gt.3302618
  56. Carmo M,  Peixoto CC, Coroadinha AS, Alves PM, Cruz PE, Carrondo MJ, 2004. Quantitation of MLV-based retroviral vectors using real-time RT-PCR. J Virol Methods, 119:115-9. http://dx.doi.org/10.1016/j.jviromet.2004.03.007
  57. Cruz PE, L Maranga, MJ Carrondo, 2002. Integrated process optimization: lessons from retrovirus and virus-like particle production. J Biotechnol, 99:199-214. http://dx.doi.org/10.1016/S0168-1656(02)00210-9
  58. Merten OW, Cruz PE, Rochette C, Geny-Fiamma C, Bouquet C, Goncalves D, Danos O, Carrondo MJ, 2001. Comparison of different bioreactor systems for the production of high titer retroviral vectors. Biotechnol Prog, 17:326-35. http://dx.doi.org/10.1021/bp000162z
  59. Morais VA, Serpa J, Palma AS, Costa T, Maranga L, Costa J, Wu N, Ataai MM, 2001. Expression and characterization of recombinant human alpha-3/4-fucosyltransferase III from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) cells using the baculovirus expression system. Biochem J, 353:719-25. http://www.biochemj.org/bj/353/bj3530719.htm
  60. Cruz PE, Almeida JS, Murphy PN, Moreira JL, Carrondo MJ, 2000. Modeling retrovirus production for gene therapy. 1. Determination of optimal bioreaction mode and harvest strategy. Biotechnol Prog, 16:213-21. http://dx.doi.org/10.1021/bp9901466
  61. Cruz PE, Goncalves D, Almeida J, Moreira JL, Carrondo MJ, 2000. Modeling retrovirus production for gene therapy. 2. Integrated optimization of bioreaction and downstream processing. Biotechnol Prog, 16:350-57. http://dx.doi.org/10.1021/bp000017l
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