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Recombinant Proteins

  1. Sequeira DP, Correia R, Carrondo MJT, Roldao A, Teixeira AP, Alves PM.  2017.  Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production.  Vaccine. pii: S0264-410X(17)30246-3. http://dx.doi.org/10.1016/j.vaccine.2017.02.043
  2. Isidro IA, Portela RM, Clemente JJ, Cunha AE, Oliveira R.  2016.  Hybrid metabolic flux analysis and recombinant protein prediction in Pichia pastoris X-33 cultures expressing a single-chain antibody fragment.  Bioprocess Biosyst Eng.  39(9):1351-63. https://doi.org/10.1007/s00449-016-1611-z
  3. Patrone M, Coroadinha AS, Teixeira AP, Alves PM. 2016. Palmitoylation strengthens cholesterol-dependent multimerization and fusion activity of human cytomegalovirus gB. Journal of Biochemical Chemistry (in press). http://dx.doi.org/10.1074/jbc.M115.682252
  4. Alves, M., Lamego, J., Bandeiras, T., Castro, R., Tomas, H., Coroadinha, A.S., Costa, J., and Simplício, A.L. 2016. Human Carboxylesterase 2: studies on the role of glycosylation for enzymatic activity. Biochem Biophy Rep 5, 105-10. http://dx.doi.org/10.1016/j.bbrep.2015.11.018
  5. Vlaming M, van Duijn E, Dillingh MR, Brands R, Windhorst AD, Hendrikse NH, Bosgra S, Burggraaf J, de Koning MC, Fidder A, Mocking J, Sandman H, de Ligt R, Fabriek BO, Pasman WJ, Seinen W, Alves T, Carrondo M, Peixoto C, Peeters P, Vaes W. 2015. Microdosing of a carbon-14 labeled protein in healthy volunteers accurately predicts its pharmacokinetics at therapeutic dosages. Clin Pharmacol Ther. Apr13. http://dx.doi.org/10.1002/cpt.131
  6. Patrone M, Carinhas N, Sousa MQ, Peixoto C, Ciferri C, Carfì A, Alves PM. 2014. Enhanced expression of full-length human cytomegalovirus fusion protein in non-swelling baculovirus-infected cells with a minimal fed-batch strategy. PloS One. http://dx.doi.org/10.1371/journal.pone.0090753 
  7. Fernandes F, Dias MM, Vidigal J, Patrone M, Teixeira AP, Alves PM. 2014. Production of rotavirus core-like particles in Sf9 cells using recombinase-mediated cassette exchange. J Biotechnol. 71: 34–38
  8. http://dx.doi.org/10.1016/j.jbiotec.2013.11.020
  9. Carinhas N, Duarte TM, Barreiro LC, Carrondo MJT, Alves PM, Teixeira AP. 2013. Metabolic signatures of GS‑CHO cell clones associated with butyrate treatment and culture phase transition. Biotechnol Bioeng, 110(12), 3244–3257. http://dx.doi.org/10.1002/bit.24983/
  10. Fernandes F, Teixeira AP, Carinhas N, Carrondo MJT, Alves PM. 2013. Insect cells as production platform of complex VLPs. Expert Rev Vaccines. Feb;12(2):225-36. http://dx.doi.org/10.1586/erv.12.153
  11. Simplício AL, Coroadinha AS, Gilmer JF, Lamego J. 2013. A methodology for detection and quantification of esterase activity. Methods Mol Biol. 2013;984:309-19. http://dx.doi.org/10.1007/978-1-62703-296-4_22
  12. Lamego J, Cunha B, Peixoto C, Sousa MF, Alves PM, Simplicio AL, Coroadinha AS, 2013. Carboxylesterase 2 production and characterization in human cells: new insights into enzyme oligomerization and activity. Appl Microbiol Biotechnol. Feb;97(3):1161-73 http://dx.doi.org/10.1007/s00253-012-3994-3.
  13. Fernandes F, Vidigal J, Dias M, Prather KL, Coroadinha AS, Teixeira AP, Alves PM, 2012. Flipase-mediated cassette exchange in Sf9 insect cells for stable gene expression. Biotechnol Bioeng, 109(11):2836-44. http://dx.doi.org/10.1002/bit.24542
  14. Lamego J, Coroadinha AS, Simplicio AL, 2011. Detection and Quantification of Carboxylesterase 2 Activity by Capillary Electrophoresis. Anal Chem, 83(3):881–887. http://dx.doi.org/10.1021/ac102547c
  15. Franco CF, Mellado MC, Alves PM, Coelho AV, 2010. Monitoring virus-like particle and viral protein production by intact cell MALDI-TOF mass spectrometry. Talanta, 80(4):1561-8. http://dx.doi.org/10.1016/j.talanta.2009.06.081
  16. Vieira HL, Pereira AC, Peixoto C, Moraes RH, Alves PM, Mendonça RZ, 2010. Improvement of recombinant protein production by an anti-apoptotic protein from hemolymph of Lonomia obliqua. Cytotechnology, 62(6):547-55. http://dx.doi.org/10.1007/s10616-010-9305-x
  17. Roldão A, Oliveira R, Carrondo MJ, Alves PM, 2009. Error Assessment in Recombinant Baculovirus Titration: Evaluation of Different Methods. J Virol Methods, 159:69-80. http://dx.doi.org/10.1016/j.jviromet.2009.03.007
  18. Vieira HL, Cunha L, Goldmacher VS, Alves PM, 2009. The effect of the cell death suppressor vMIA on the production of a recombinant protein in the adenovirus-293 expression system. Protein Expr Purif, 64(2):179-84. http://dx.doi.org/10.1016/j.pep.2008.11.004
  19. Gouveia RM, Gomes CM, Sousa MF, Alves, PM, Costa J, 2008. Kinetic analysis of L1 Homophilic Interaction: Role of the First Four Ig Domains and Implications on L1-L1 Binding Mechanism. J Biol Chem, 283(42):28038-47. http://dx.doi.org/10.1074/jbc.M804991200
  20. Mellado MC, Peixoto C, Cruz PE, Carrondo MJ, Alves PM, 2008. Purification of recombinant rotavirus VP7 for the study of in vitro rotavirus-like particles assembly. J Chromatogr B, 874:89-94. http://dx.doi.org/10.1016/j.jchromb.2008.09.002
  21. Bento I, Peixoto C, Zaitsev VN, Lindley P, 2007. Ceruloplasmin revisited: structural and functional roles of various metal cation-binding sites. Acta Crystallogr D Biol Crystallogr, 63(2):240-48. http://dx.doi.org/10.1107/S090744490604947X
  22. Teixeira A, Alves C, Alves PM, Carrondo MJ, Oliveira R, 2007. Hybrid elementary flux analysis/nonparametric modeling: application for bioprocess control. BMC Bioinformatics, 8(1):30. http://dx.doi.org/10.1186/1471-2105-8-30
  23. Teixeira AP, Carinhas N, Dias JM, Cruz PE, Alves PM, Carrondo MJ, Oliveira R, 2007. Hybrid semi-parametric mathematical systems: bridging the gap between biology and process engineering. J Biotechnol, 132:418-25. http://dx.doi.org/10.1016/j.jbiotec.2007.08.020
  24. Wirth D, Gama-Norton L, Riemer P, Sandhu U, Schucht R, Hauser H, 2007. Road to precision: recombinase-based targeting technologies for genome engineering. Curr Opin Biotechnol, 18:411–19. http://dx.doi.org/10.1016/j.copbio.2007.07.013
  25. Gouveia RM, Morais VA, Peixoto C, Sousa MF, Regalla M, Alves PM, Costa J, 2007. Production and purification of functional truncated soluble forms of human recombinant L1 cell adhesion glycoprotein from Spodoptera frugiperda Sf9 cells. Protein Expr and Purif, 52:182-93. http://dx.doi.org/10.1016/j.pep.2006.10.008
  26. Vieira HL, Pereira ACP, Carrondo MJ, Alves PM, 2006. Catalase effect on apoptosis for the improvement of recombinant protein production in baculovirus-insect cell system. Bioprocess Biosyst Eng, 29(5-6):409-14. http://dx.doi.org/10.1007/s00449-006-0093-9
  27. Teixeira AP, Clemente JJ, Cunha AE, Carrondo MJ, Oliveira R, 2006. Bioprocess iterative batch-to-batch optimization based on hybrid parametric/nonparametric models. Biotechnol Prog, 22(1):247-58. http://dx.doi.org/10.1021/bp0502328
  28. Lameiro MH, Lopes A, Martins LO, Alves PM, Melo E, 2006. Incorporation of a Model Protein into Chitosan-Bile salt Microparticles. Int J Pharm, 312:119-30. http://dx.doi.org/10.1016/j.ijpharm.2006.01.006
  29. Teixeira A, Cunha AE, Clemente JJ, Moreira JL, Cruz HJ, Alves PM, Carrondo MJ, Oliveira R, 2005. Modelling and optimization of a recombinant BHK-21 cultivation process using hybrid grey-box systems. J Biotechnol, 118:290-303. http://dx.doi.org/10.1016/j.jbiotec.2005.04.024
  30. Carvalhal AV, Marcelino I, Carrondo MJ, 2003. Metabolic changes during cell growth inhibition by p27 overexpression. Appl Microbiol Biotechnol, 63:164-73. http://dx.doi.org/10.1007/s00253-003-1385-5
  31. Carvalhal A, Coroadinha AS, Alves PM, Moreira JL, Hauser H, Carrondo MJ, 2002. Metabolic changes during cell growth inhibition by the IRF-1 system. Enzyme Microb Technol, 30:95-109. http://dx.doi.org/10.1016/S0141-0229(01)00460-4
  32. Cruz HJ, Conradt HS, Dunker R, Peixoto CM, Cunha AE, Thomaz M, Burger C, Dias EM, Clemente J, Moreira JL, Reike E, Carrondo MJ, 2002. Process development of a recombinant antibody/interleukin-2 fusion protein expressed in protein-free medium by BHK cells. J Biotechnol, 96:169-83. http://dx.doi.org/10.1016/S0168-1656(02)00028-7
  33. Cruz PE, L Maranga, MJ Carrondo, 2002. Integrated process optimization: lessons from retrovirus and virus-like particle production. J Biotechnol, 99:199-214. http://dx.doi.org/10.1016/S0168-1656(02)00210-9
  34. Maranga L, PE Cruz, JG Aunins, MJ Carrondo, 2002. Production of core and virus-like particles with baculovirus infected insect cells. Adv Biochem Eng Biotechnol, 74:183-206. http://dx.doi.org/10.1007/3-540-45736-4_9
  35. Maranga L, Rueda P, Antonis AF, Vela C, Langeveld JP, Casal JI, Carrondo MJ, 2002. Large scale production and downstream processing of a recombinant porcine parvovirus vaccine. Appl Microbiol Biotechnol, 59:45-50. http://dx.doi.org/10.1007/s00253-002-0976-x
  36. Carvalhal AV, Moreira JL, Carrondo MJ, 2001. Strategies to modulate BHK cell proliferation by the regulation of IRF-1 expression. J Biotechnol, 92:47-59. http://dx.doi.org/10.1016/S0168-1656(01)00365-0
  37. Morais VA, Serpa J, Palma AS, Costa T, Maranga L, Costa J, Wu N, Ataai MM, 2001. Expression and characterization of recombinant human alpha-3/4-fucosyltransferase III from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) cells using the baculovirus expression system. Biochem J, 353:719-25. http://www.biochemj.org/bj/353/bj3530719.htm
  38. Carvalhal AV, Moreira JL, Carrondo MJ, 2001. Strategies to modulate BHK cell proliferation by the regulation of IRF-1 expression. J Biotechnol, 92:47-59. http://dx.doi.org/10.1016/S0168-1656(01)00365-0
  39. Cruz HJ, Dias EM, Peixoto CM, Moreira JL, Carrondo MJ, 2000. Product quality of a recombinant fusion protein expressed in immobilised baby hamster cells grown in protein-free medium. Biotechnol Lett, 22:677-82. http://dx.doi.org/10.1023/A:1005631602517
  40. Cruz HJ, Peixoto CM, Nimtz M, Alves PM, Dias EM, Moreira JL, Carrondo MJ, 2000. Metabolic shifts do not influence the glycosylation patterns of a recombinant fusion protein expressed in BHK cells. Biotechnol Bioeng, 69:129-39. http://dx.doi.org/10.1002/(SICI)1097-0290(20000720)69:2<129::AID-BIT2>3.0.CO;2-5
  41. Burger C, Carrondo MJ, Cruz H, Cuffe M, Dias E, Griffiths JB, Hayes K, Hauser H, Looby D, Mielke C, Moreira JL, Rieke E, Savage AV, Stacey GN, Welge T, 1999. An integrated strategy for the process development of a recombinant antibody-cytokine fusion protein expressed in BHK cells. Appl Microbiol Biotechnol, 52:345-53. http://dx.doi.org/10.1007/s002530051530
  42. Cruz PE, Martins PC, Alves PM, Peixoto CC, Santos H, Moreira JL, Carrondo MJ,  1999. Proteolytic activity in infected and non infected insect cells: degradation of HIV-1 Pr55gag particles. Biotechnol Bioeng, 65:133-43. http://dx.doi.org/10.1002/(SICI)1097-0290(19991020)65:2%3C133::AID-BIT2%3E3.0.CO;2-X
  43. Cruz HJ, Moreira JL, Stacey G, Dias EM, Hayes K, Looby D, Griffiths B, Carrondo MJ, 1998. Adaptation of BHK cells producing a recombinant protein to serum-free media and protein-free medium. Cytotechnology, 26:59-64. http://dx.doi.org/10.1023/A:1007951813755
  44. Cruz PE, Peixoto CC, Moreira JL, Carrondo MJ, 1998. Optimization of the production of virus-like particles in insect cells. Biotechnol Bioeng, 60:408-18. http://dx.doi.org/10.1002/(SICI)1097-0290(19981120)60:4<408::AID-BIT2>3.0.CO;2-Q
  45. Cruz PE, Peixoto CC, Moreira JL, Carrondo MJ, 1998. Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells. J Chem Technol Biotechnol, 72:149-58. http://dx.doi.org/10.1002/(SICI)1097-4660(199806)72:2%3C149::AID-JCTB886%3E3.3.CO;2-3
  46. Racher AJ, Moreira JL, Alves PM, Wirth M, Weidle UH, Hauser H, Carrondo MJ, Griffiths JB, 1994. Expression of recombinant antibody and secreted alkaline phosphatase in mammalian cells. Influence of cell line and culture system upon production kinetics. Appl Microbiol Biotechnol, 40:851-6.  http://dx.doi.org/10.1007/BF00173987
  47. Moreira JL, Wirth M, Fitzek M, Hauser H, 1992. Evaluation of reporter genes in mammalian cell lines. Meth Mol Cell Biol, 3:23-2.
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