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Vaccines

  1. Monteiro F, Bernal V, Alves PM. 2017. Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production. BIOTECHNOLOGY AND BIOENGINEERING.  114, 674-684.  http://dx.doi.org/10.1002/bit.26089
  2. Sequeira DP, Correia R, Carrondo MJT, Roldao A, Teixeira AP, Alves PM.  2017.  Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production.  Vaccine. pii: S0264-410X(17)30246-3. http://dx.doi.org/10.1016/j.vaccine.2017.02.043
  3. Patrone M, Coroadinha AS, Teixeira AP, Alves PM. 2016. Palmitoylation strengthens cholesterol-dependent multimerization and fusion activity of human cytomegalovirus gB. Journal of Biochemical Chemistry. http://dx.doi.org/10.1074/jbc.M115.682252
  4. Silva AC, Teixeira AP, Alves PM. 2016. Impact of Adenovirus infection in host cell metabolism evaluated by 1H NMR spectroscopy. J Biotechnol. http://dx.doi.org/10.1016/j.jbiotec.2016.05.025
  5. Soares HR, Castro R, Tomas HA, Rodrigues AF, Gomes-Alves P, Bellier B, Klatzmann D, Carrondo MJ, Alves PM, Coroadinha AS. 2016. Tetraspanins displayed in retrovirus-derived virus-like particles and their immunogenicity. Vaccine. http://dx.doi.org/10.1016/j.vaccine.2015.12.015
  6. Silva AC, Simão D, Sousa MFQ, Küppers C, Lucas T, Cruz P, Carrondo MJ,  Kochanek S, Alves PM 2015 Human Amniocyte-derived Cells for the Production of Adenoviral Vectors. Biotechnology Journal, http://dx.doi.org/10.1002/biot.201400765
  7. Castro R, Fernandes P, Laske T, Sousa MFQ, Genzel Y, Scharfenberg K, Alves PM, Coroadinha AS. 2015. Production of canine adenovirus type 2 in serum-free suspension cultures of MDCK cells. Appl Microbiol Biotechnol http://dx.doi.org/10.1007/s00253-015-6636-8
  8. Silva AC, Yami M, Libeau G, Carrondo MJ, Alves PM. 2014. Testing a new formulation for Peste des Petits Ruminants vaccine in Ethiopia. Vaccine, 32(24): 2878–81  http://dx.doi.org/10.1016/j.vaccine.2014.02.039 
  9. Patrone M, Carinhas N, Sousa MQ, Peixoto C, Ciferri C, Carfì A, Alves PM. 2014. Enhanced expression of full-length human cytomegalovirus fusion protein in non-swelling baculovirus-infected cells with a minimal fed-batch strategy. PloS One. http://dx.doi.org/10.1371/journal.pone.0090753
  10. Fernandes F, Dias MM, Vidigal J, Sousa MF, Patrone M, Teixeira AP, Alves PM. 2014. Production of rotavirus core-like particles in Sf9 cells using recombinase-mediated cassette exchange. J Biotechnol. http://dx.doi.org/10.1016/j.jbiotec.2013.11.020
  11. Fernandes F, Teixeira AP, Carinhas N, Carrondo MJT, Alves PM. 2013. Insect cells as production platform of complex VLPs. Expert Rev Vaccines. Feb;12(2):225-36.  http://dx.doi.org/10.1586/erv.12.153
  12. Marcelino I, Almeida AM, Brito C, Meyer D, Barreto M, Sheikboudou C, Franco CF, Martinez D, Lefrançois T, Vachiéry N, Carrondo MJ, Coelho A, Alves PM, 2012. Proteomic analyses of Ehrlichia ruminantium highlight differential expression of MAP1-family proteins. Vet Microbiol, 156 (2012) 305–314. http://dx.doi.org/10.1016/j.vetmic.2011.11.022
  13. Rodrigues AF, Guerreiro MR, Santiago VM, Dalba C, Klatzmann D, Alves PM, Carrondo MJ, Coroadinha AS, 2011. Down-regulation of CD81 tetraspanin in human cells producing retroviral-based particles: tailoring vector composition. Biotechnol Bioeng, 108(11):2623-33. http://dx.doi.org/10.1002/bit.23231
  14. Silva AC, Carrondo MJ, and Alves PM, 2011. Strategies for improved stability of Peste des Petits Ruminants vaccine. Vaccine, 29:4983-91. http://dx.doi.org/10.1016/j.vaccine.2011.04.102
  15. Roldão A, Mellado MC, Castilho LR, Carrondo MJ, Alves PM, 2010. Virus-like particles in vaccine development. Expert Rev Vaccines, 9(10):1149-76. http://dx.doi.org/10.1586/erv.10.115
  16. Matias AA, Serra AT, Silva AC, Perdigão R, Ferreira TB, Marcelino I, Silva S, Coelho AV, Alves PM, Duarte CM, 2010. Portuguese winemaking residues as a potential source of natural anti-adenoviral agents.Int. J Food Sci Nutr, 61(4):357-68. http://dx.doi.org/10.3109/09637480903430990
  17. Adakal H, Stachurski F, Konkobo M, Zoungrana S, Meyer DF, Pinarello V, Aprelon R, Marcelino I, Alves PM, Martinez D, Lefrancois T, Vachiéry N, 2010. Efficiency of inactivated vaccines against heartwater in Burkina Faso: Impact of Ehrlichia ruminantium genetic diversity. Vaccine, 28(29):4573-80. http://dx.doi.org/10.1016/j.vaccine.2010.04.087
  18. Mellado MC, Mena JA, Lopes A, Ramirez OT, Carrondo MJ, Palomares LA, Alves PM, 2009. Impact of physicochemical parameters on in vitro assembly and disassembly kinetics of recombinant triple-layered rotavirus-like particles. Biotechnol Bioeng, 104(4):674-86. http://dx.doi.org/10.1002/bit.22430
  19. Ferreira TB, Perdigão R, Silva AC, Zhang C, Aunins JG, Carrondo MJ, Alves PM, 2009. 293 cell cycle synchronisation in adenovirus vector production. Biotechnol Prog, 25(1):235-43. http://dx.doi.org/10.1002/btpr.64
  20. Mellado MC, Peixoto C, Cruz PE, Carrondo MJ, Alves PM, 2008. Purification of recombinant rotavirus VP7 for the study of in vitro rotavirus-like particles assembly. J Chromatogr B, 874:89-94. http://dx.doi.org/10.1016/j.jchromb.2008.09.002
  21. Roldão A, Carrondo MJ, Alves PM, Oliveira, R, 2008. Stochastic simulation of protein expression in the Baculovirus/Insect cell system. Comp Chem Eng, 32:68-77. http://dx.doi.org/10.1016/j.compchemeng.2007.04.017
  22. Silva AC, Delgado I, Sousa MF, Carrondo MJ, Alves PM, 2008. Scalable culture systems using different cell lines for the Production of Peste des Petits Ruminants Vaccine. Vaccine, 26:3305-11. http://dx.doi.org/10.1016/j.vaccine.2008.03.077
  23. Mellado MC, Franco C, Coelho AV, AlvesPM, Simplício AL, 2008. Sodium dodecyl sulfate-capillary gel electrophoresis analysis of rotavirus-like particles. J Chromatogr A, 1192:166-72. http://dx.doi.org/10.1016/j.chroma.2008.03.035
  24. Vicente T, Sousa MF, Peixoto C, Mota JP, Alves PM, Carrondo MJ, 2008. Anion exchange membrane chromatography for purification of rotavirus-like particles. J Membr Sci, 311:270-83. http://dx.doi.org/10.1016/j.memsci.2007.12.021
  25. Ferreira TB, Carrondo MJ, Alves PM, 2007. Effect of ammonia production on intracellular pH consequent effect on adenovirus vector production. J Biotechnol, 129(3):433-38. http://dx.doi.org/10.1016/j.jbiotec.2007.01.010
  26. Peixoto C, Sousa MF, Silva AC, Carrondo MJ, Alves PM, 2007. Downstream processing of triple-layered rotavirus like particles. J Biotechnol, 127:452-61 http://dx.doi.org/10.1016/j.jbiotec.2006.08.002
  27. Peixoto C, Marcelino I, Amaral AI, Carrondo MJ, Alves PM, 2007. Purification by membrane technology of an intracellular Ehrlichia ruminantium candidate vaccine against heartwater. Proc Biochem, 42(7):1084-89. http://dx.doi.org/10.1016/j.procbio.2007.04.012
  28. Roldão A, Vieira HL, Charpilienne A, Poncet D, Roy P, Carrondo MJ, Alves PM, Oliveira R, 2007. Modeling rotavirus-like particles production in a baculovirus expression vector system: infection kinetics, baculovirus DNA replication, mRNA synthesis and protein production. J Biotechnol, 128(4):875-94. http://dx.doi.org/10.1016/j.jbiotec.2007.01.003
  29. Faburay B, Geysen D, Ceesay A, Marcelino I, Alves PM, Taoufik A, Postigo M, Bell-Sakyi L, Jongejan F, 2007. Immunisation of sheep against heartwater in The Gambia using inactivated and attenuated Ehrlichia ruminantium vaccines. Vaccine, 25(46):7939-47. http://dx.doi.org/10.1016/j.vaccine.2007.09.002
  30. Marcelino I, Vachiéry N, Amaral AI, Roldão A, Lefrançois T, Carrondo MJ, Alves PM, Martinez D, 2007. Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater. Vaccine, 25(26):4903-13. http://dx.doi.org/10.1016/j.vaccine.2007.04.055
  31. Lameiro MH, Malpique R, Silva AC, Alves PM, Melo E, 2006. Encapsulation of adenoviral vectors into chitosan-bile salt microparticles for mucosal vaccination, J Biotechnol, 126:152-62. http://dx.doi.org/10.1016/j.jbiotec.2006.04.030
  32. Marcelino I, Sousa MF, Veríssimo C, Cunha A, Carrondo MJ, Alves PM, 2006. Process development for the mass production of Ehrlichia ruminantium. Vaccine, 24(10):1716-25. http://dx.doi.org/10.1016/j.vaccine.2005.08.109
  33. Roldão A, Vieira HL, Alves PM, Oliveira R, Carrondo MJ, 2006. Intracellular dynamics in rotavirus-like particles production: Evaluation of multigene and monocistronic infection strategies. Proc Biochem, 41:2188-99. http://doi:10.1016/j.procbio.2006.06.019
  34. Peixoto C, Ferreira TB, Carrondo MJ, Cruz PE, Alves PM, 2006. Purification of adenoviral vectors using expanded bed chromatography. J Virol Methods, 132:121-6. http://dx.doi.org/10.1016/j.jviromet.2005.10.002
  35. Zhang C, Ferreira TB, Cruz PE, Alves PM, Haury M, Carrondo MJ, 2006. The importance of 293 cell cycle phase on adenovirus vector production, Enzyme Microb Technol. 39:1328-32. http://dx.doi.org/10.1016/j.enzmictec.2006.03.021
  36. Antonis AF, Bruschke CJ, Rueda P, Maranga L, Casal JI, Vela C, Hilgers LA, Belt PB, Weerdmeester K, Carrondo MJ, Langeveld JP, 2006. A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure. Vaccine, 24(26):5481-90. http://dx.doi.org/10.1016/j.vaccine.2006.03.089
  37. Ferreira TB, Ferreira AL, Carrondo MJ, Alves PM, 2005. Effect of refeed strategies and non-ammoniagenic medium on adenovirus production at high cell densities. J Biotechnol, 119:272-80. http://dx.doi.org/10.1016/j.jbiotec.2005.03.009
  38. Vieira HL, Estevão C, Roldão A, Peixoto CC, Sousa MF, Cruz PE, Carrondo MJ, Alves PM, 2005. Triple layered rotavirus VLP assembly: kinetics of vector replication, mRNA stability and recombinant protein production. J Biotechnol, 120(1):72-82. http://dx.doi.org/10.1016/j.jbiotec.2005.03.026
  39. Ferreira TB, Alves PM, Aunins JG, Carrondo MJ, 2005. Use of adenoviral vectors as veterinary vaccines. Gene Ther, 12(S1):73-83. http://dx.doi.org/10.1038/sj.gt.3302618
  40. Ferreira TB, Ferreira AL, Carrondo MJ, Alves PM, 2005. Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production. Biotechnol Lett, 27(22): 1809-13. http://dx.doi.org/10.1007/s10529-005-3558-z
  41. Peixoto CC, Marcelino I, Vachiéry N, Bensaid A, Martinez D, Carrondo MJ, Alves PM, 2005. Quantification of Ehrlichia ruminantium by real time PCR. Vet Microbiol, 107:273-8. http://dx.doi.org/10.1016/j.vetmic.2005.02.001
  42. Marcelino I, Veríssimo C, Sousa MF, Carrondo MJ, Alves PM, 2005. Characterization of Ehrlichia ruminantium replication and release kinetics in endothelial cell cultures. Vet Microbiol, 110:87-96. http://dx.doi.org/10.1016/j.vetmic.2005.07.012
  43. Maranga L, Brazão TF, Carrondo MJ, 2003. Virus like particles production at low multiplicities of infection with the baculovirus insect cell system. Biotechnol  Bioeng, 84:245-53. http://dx.doi.org/10.1002/bit.10773
  44. Maranga L, Mendonca RZ, Bengala A, Peixoto CC, Moraes RH, Pereira CA, Carrondo MJ, 2003. Enhancement of Sf-9 cell growth and longevity through supplementation of culture medium with hemolymph. Biotechnol Prog, 19:58-63. http://dx.doi.org/10.1021/bp025583q
  45. Cruz PE, L Maranga, MJ Carrondo, 2002. Integrated process optimization: lessons from retrovirus and virus-like particle production. J Biotechnol, 99:199-214. http://dx.doi.org/10.1016/S0168-1656(02)00210-9
  46. Maranga L, Rueda P, Antonis AF, Vela C, Langeveld JP, Casal JI, Carrondo MJ, 2002. Large scale production and downstream processing of a recombinant porcine parvovirus vaccine. Appl Microbiol Biotechnol, 59:45-50. http://dx.doi.org/10.1007/s00253-002-0976-x
  47. Maranga L, PE Cruz, JG Aunins, MJ Carrondo, 2002. Production of core and virus-like particles with baculovirus infected insect cells. Adv Biochem Eng Biotechnol, 74:183-206. http://dx.doi.org/10.1007/3-540-45736-4_9
  48. Cruz PE, Peixoto CC, Devos K, Moreira JL, Saman E, Carrondo MJ, 2000. Characterization and downstream processing of HIV-1 core and virus-like-particles produced in serum free medium. Enzyme Microb Technol, 26:61-70. http://dx.doi.org/10.1016/S0141-0229(99)00128-3
  49. Cruz PE, Martins PC, Alves PM, Peixoto CC, Santos H, Moreira JL, Carrondo MJ, 1999. Proteolytic activity in infected and non infected insect cells: degradation of HIV-1 Pr55gag particles. Biotechnol Bioeng, 65:133-43. http://dx.doi.org/10.1002/(SICI)1097-0290(19991020)65:2%3C133::AID-BIT2%3E3.0.CO;2-X
  50. Cruz PE, Peixoto CC, Moreira JL, Carrondo MJ, 1998. Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells. J Chem Technol Biotechnol, 72:149-58. http://dx.doi.org/10.1002/(SICI)1097-4660(199806)72:2%3C149::AID-JCTB886%3E3.3.CO;2-3
  51. Cruz PE, Peixoto CC, Moreira JL, Carrondo MJ, 1998. Optimization of the production of virus-like particles in insect cells. Biotechnol Bioeng, 60:408-18. http://dx.doi.org/10.1002/(SICI)1097-0290(19981120)60:4<408::AID-BIT2>3.0.CO;2-Q
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