[SCAN] Exploring bacterial transcriptomes to unravel RNA regulation and discover new RNAs
Vânia Pobre
| When |
04 Nov, 2020
from
12:00 pm to 01:00 pm |
|---|---|
| Contact Name | Rita Abranches |
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Title: Exploring bacterial transcriptomes to unravel RNA regulation and discover new RNAs
Speaker: Vânia Pobre
Abstract: Bacteria can survive in the most diverse environments by readjusting their transcriptome. This rearrangement involves the rapid regulation of the degradation and synthesis of RNAs in the cells. Two classes of RNA regulators are the ribonucleases, that mature and degrade RNAs, and small non-coding RNAs (sRNAs), that regulate gene expression at the transcriptional and post-transcriptional levels. High-throughput sequencing (RNA-Seq) allows us to determine the transcriptome and the massive amounts of data obtained can be applied in a variety of studies. I will give two examples of uses for RNA-Seq data. Firstly, I will present how we used RNA-Seq to discover new regulatory roles of the three main Escherichia coli exoribonucleases, RNase II, RNase R and PNPase. By performing differential RNA-Seq we found that the exoribonucleases affect E. coli motility, biofilm formation, responses to different stress conditions and even the transition between exponential and stationary phases. I will also show how we identified novel sRNAs using RNA-Seq data from the biotechnological important bacteria Herbaspirillum seropedicae and Pseudomonas putida. We were able to confirm the expression of 117 novel sRNAs in H. seropedicae and the expression of 725 novel sRNAs in P. putida. These examples of RNA-Seq data usage demonstrate how new technological advances are important to discover new RNAs and to increase the understanding of RNA regulation.
ITQB NOVA Virtual Auditorium
