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Lab Members

Lígia O Martins, Principal Investigator, Head of Lab

lmartins@itqb.unl.pt


 

FORMER MEMBERS

 

 

Post-doctoral

Sónia Mendes May15-Nov17

Structure-function studies in bacterial Dye-decolourising peroxidases and Pyranose 2-oxidase. Sónia is currently Senior Research Associate at the Bioprocess Development and Analytics division at iBET.

 

Bruno Reynolds Jan 10-Oct 13

Setting up a Technology Transfer Office in the Universidade Nova de Lisboa. Co-supervision with Nuno Arantes de Oliveira, Alfama. Bruno is now a staff member of ISIS Innovation: Technology Tranfer of Oxford University, UK.

 

Zhenjhia Chen Nov 06-Feb 2013

Spectroscopic and kinetic characterization of site-directed mutants of CotA-laccase from Bacillus subtilis. Chen is presently the Deputy Director of Coffee Engineering Research Center of China, Padi Industrial Park, Mangshi City, Dehong Autonomous Prefecture, Yunnan, China.

 

Luciana Pereira April 05- Dec08

Evaluation of CotA-laccase towards degradation and synthesis of industrial dyes. Luciana is presently a Senior Researcher at Dep. Engenharia Biológica, Univ Minho.

 

Isabel Bento June 04-06

Structure-function relationships of prokaryotic laccases. Co-supervision with Prof Peter Lindley. Isabel is currently at European Biology Lab, EMBL, Heidelberg

 


PhD students

Ana Fernandes 2015- 2019

Cooperative organometallic and enzymatic catalysis to develop new value chains from organic wastes. PhD in Sustainable Chemistry. Co-Supervision with Beatriz Royo, ITQB.

 

Ana Catarina Sousa PhD May 2015

Biotransformation of aromatic amines by laccases, IST-UL. Co-Supervision with Maria Paula Robalo, ISEL/IST. Catarina is presently an Assistant Professor at ISEL

 

Sónia Mendes PhD April 2015

Multi-enzymatic systems for cleaning-up synthetic dyes from the environment. Sónia is currently Senior Research Associate at the Bioprocess Development and Analytics division at iBET

 

André T. Fernandes PhD February 2011

Insight into Multicopper Oxidases Stability. André is currently the Business Development Manager at “Bioline”; previously was the Head of Sales Division of the company “NZYtech genes & enzymes“

 

Paulo Durão PhD March 2010

CotA-laccase from Bacillus subtilis: Structure-Function Studies of Catalytic Copper Centers. Paulo is currently a Post-doc at Isabel Gordo Lab at IGC and before was in Franz-Ulrich Hartl’s Lab at Max-Planck Inst of Biochemistry, Martinsried, Munich, Germany

 


Master students

Francisco Nunes, Sep18-Sep19

Isoeugenol monooxygenase characterization and whole-cell bioconversion systems towards vanillin production from plant-derived precursor isoeugenol. MsC in Applied Microbiology, Faculdade de Ciências, Universidade de Lisboa.

 

André Taborda, Set18-Sep19

Development of novel bacterial enzymesfor medical diagnosis through directed evolution. MsC in Applied Microbiology, Faculdade de Ciências, Universidade de Lisboa.

 

André Faria, Jan-Nov18

Droplet microfluidic systems for directed evolution: aiming at integration. Co-supervision: João Pedro Conde, IST and INESC, Lisboa. MsC in Bioengineering and Nanosystems, Instituto Superior Técnico, Univ Lisboa.

 

Diana Santos, Sep16-Oct17

Improving a bacterial pyranose 2-oxidase from Arthrobacter siccitolerans through directed evolution. MsC in Biochemistry for Health, Faculdade de Ciências, Universidade de Lisboa.

 

Guillherme Andrade, Apr16-Dec17

Droplet microfluidic systems for directed evolution. Co-supervision: João Pedro Conde, IST and INESC, Lisboa. MsC in Bioengineering and Nanosystems, Instituto Superior Técnico, Univ Lisboa.

 

Lorenzo Benardo, March-Dec17

Development of multienzymatic systems towards the valorization of coloured textile effluents. Co-supervision: Giovanna Cristina Varese. MsC in Plant Biotecnology, Universitá degli Studi di Torino, Italy.

 

Joaquim Madeira, Feb14–Jul15

Bacterial Hydrogen Peroxide Forming Enzymes. MsC in Biological Engineering, Universidade do Algarve.

 

Bruna Pinto, Feb14 – Feb15

Biotransformation of toxic aromatic amines. Co-supervision: Maria Paula Robalo, ISEL and IST, Lisboa. MsC in Chemical Engineering, Instituto Superior de Engenharia de Lisboa.

 

Diogo Tavares, Sep13-Nov14

Engineering new bacterial dye-decolourising peroxidases for lignin degradation. Co-supervision: Vânia Brissos. MsC in Applied Microbiology, Faculdade de Ciências, Universidade de Lisboa.

 

Ana Filipa Santos  Feb 12-Dec12

Kinetic and spectroscopic characterization of a DyP-type peroxidase from Bacillus subtilis. MsC in Biological Engineering, Instituto Superior Técnico, Univ Lisboa.

 

Nádia Gonçalves Sep11-Sep12

Evolving the thermostability of PpAzoR, an azoredutase from Pseudomonas putida MET94, by directed evolution. Co-supervision: Vânia Brissos. Master in Biochemistry, Faculdade de Ciências e Tecnologia, Universidade de Coimbra

 


PostGraduate students

Magdalena Lejmer, (Poland), Jul 18- Sep19

Directed evolution of hyperthermophilic McoA towards improved efficiency for lignin-related phenolics. Co-supervision: Vânia Brissos

 

Joana Pedroso (FF-UP), Jul 18- Sep 19

Crystallographic studies of bacterial DyPs and Pyranose 2-oxidase. Co-supervision: Carlos Frazão

 

Diana Santos (FCUL), Jan 18 – Dec 18

Directed evolution of AsP2Ox a bacterial pyranose 2-oxidase

 

Zuleica Duarte (FCT NOVA & IST), Nov 17 – May18

Engineering the hyperthermophilic McoA laccase for improved oxidation of phenolics. Co-supervision: Vânia Brissos

 

Diogo Silva (Minho U), Jul 16 – Dec 17

Engineering of Arthrobacter siccitolerans galactose oxidase for improved solublity

 

Cláudio Piedade (FCUL), Feb-Jun 17

Directed evolution studies of Aquifex aeolicus McoA metalloxidase. Co-supervision: Vânia Brissos

 

Patrícia Reis (FCT-NOVA), Oct 16 – Jan 17

Degradation of lignin-model compounds using laccases and DyP peroxidases

 

Mara Lisa Marques (FCT-NOVA) May 15-Dec 16

Directed evolution studies of Aquifex aeolicus McoA. Co-supervision: Vânia Brissos

 

Lúcia Sabala (Aveiro U), June 14- May 15,

Discovery of new lignin degrading bacterial strains

 

Susana Proença (FCT-NOVA), Feb 13-Feb14,

Directed evolution of McoP, a metaloxidase from Pyrobaculum aerophilum. Co-supervision: Vânia Brissos

 

Pedro Bernardo (FCT-NOVA) July 11-Nov12,

Enzymatic transformation of lignin

 

Tânia Rosado (Évora U) July09-Dec 011,

CotA-laccase mediator systems

 

Pedro Barrulas (Évora U) July-Dec 07,

Enzymatic biotransformation of synthetic dyes. Co-supervision: Luciana Pereira

 

Mauro Conde (FC-UL), Mar-Sept 07,

Expression of CuoB de Myxococcus xanthus em E.coli

 

Sara Seixas (Lusófona U) Mar-Nov 07,

Studies on PpoA from Marinomomas mediterranea an interesting enzyme for biotech applications

 

Carlos Pinheiro (FCT-NOVA) Oct 04-Mar 05,

Microbial and enzymatic dye decolourisation

 


Undergraduate 

Francisco Sobral, Jan20-Jun20

Directed evolution of pyranose 2-oxidase from Arthrobacter siccitolerans. Co-supervision: André Taborda. Bachelor in Biology, ISA-UL.

 

Patrícia Lopes, Jan-Mar 20

Investigation of the catalytic mechanism of BsDyP a DyP peroxidase from Bacillus subtilis. Co-supervision: Teresa Catarino. Bachelor in Biochemistry, FCT NOVA.

 

Mariana Lima, Jan-Mar 19

Investigation of the catalytic mechanism of pyranose 2-oxidase from Arthrobacter siccitolerans. Co-supervision: Teresa Catarino. Bachelor in Biochemistry, FCT NOVA

 

Catalina Cepeleaga, Jan-May19

Directed evolution of the hyperthermophilic McoP metaloxidase from Pyrobaculum aerophilum. Co-supervision: Vânia Brissos. Bachelor in Cellular and Molecular Biology, FCT NOVA

 

Joana Calvário, Jan-Mar 18

Investigation of the catalytic mechanism of AsP2Ox, a bacterial pyranose 2-oxidase. Co-supervision: Teresa Catarino. Bachelor in Biochemistry, FCT NOVA

 

Adriana Inácio, Jan-May 18

Directed evolution of enzymes. Co-supervision: Vânia Brissos. Bachelor in Cellular and Molecular Biology, FCT NOVA

 

Guilherme Gouveia, Jan-Mar 18

Improving a bacterial pyranose 2-oxidase from Arthrobacter siccitolerans through directed evolution. Bachelor in Biology, FCUL

 

Manuel Carvalho, Feb-Jun17

Evolution of PpDyP from Pseudomonas putida MET94 for increased thermostability. Co-supervision: Vânia Brissos. Bachelor in Biothecnology, Universidade de Évora

 

Bernardo Rodrigues, Feb-May17

X-ray studies of Pseudomonas putida MET94 PpDyP. Co-supervision: Carlos Frazão. Bachelor in Biochemistry, FCT, NOVA

 

Catarina Banha, Feb-Jul 16

Optimization of the heterologous expression of AsP2Ox from Arthrobacter siccitolerans in Escherichia coli. Co-supervision: Sónia Mendes. Bachelor in Biotechnology, Escola Superior Agrária. Politécnico de Coimbra

 

Rita Delgado, Jan-May 16

Optimization of a new fluorescent assay to detect hydrogen peroxide. Co-supervision: Vânia Brissos. Bachelor in Biochemistry, FCT, NOVA

 

Luís Vicente, Sept-Dec 15

Engineering of Arthrobacter siccitolerans galactose oxidase for improved solublity. Co-supervision: Vânia Brissos. Bachelor in Biochemistry, FCT, NOVA

 

Antonieta Gabriel, May-Sep14

Structure-function studies in DyP peroxidases. Co-supervision: Sónia Mendes. Bachelor in Biotechnology, Escola Superior Agrária. Politécnico de Coimbra

 

Marcelo Ferreira, Jan-May14

Improving whole cell systems for Dye-type peroxidases production and immobilization. Co-supervision: Vânia Brissos. Bachelor in Biochemistry, FCT, NOVA

 

Catarina Rodrigues, Set12-Dec 12

Directed evolution of McoA, a metaloxidase from Aquifex aeolicus. Co-supervision: Vânia Brissos. Bachelor in Biochemistry, FCT, Bachelor in Biochemistry, FCT, NOVA

 

Maura Ferreira, Feb-Set12

Directed evolution of McoA, a metaloxidase from Aquifex aeolicus. Co-supervision: Vânia Brissos. Bachelor in Biochemistry, Universidade de Évora

 

Ana Filipa Santos, Feb11-July11

Expression, purification and biochemical characterization of a DyP - type peroxidase from Bacillus subtilis. Instituto Superior Técnico, Universidade de Lisboa

 

Ana Isabel Farinhas, May-Oct 10

Cloning and co-expression in Escherichia coli of ppAzoR Pseudomonas putida from and CotA-laccase from Bacillus subtilis. Co-supervision: Sónia Mendes. Bachelor in Biotechnology,Instituto Politécnico de Coimbra, Escola Superior Agrária

 

Rita Catarino, Bolsa de Investigação (BI). Oct 09-Set 10

Cloning, overproduction, purification and characterization of bacterial DyP peroxidases. Bachelor in Biochemistry, Faculdade de Ciências da Universidade de Lisboa

 

Diogo Pereira, Bolsa de Integração na Investigação (BII). Jan 09- Dec 09

Influence of aromatic substitution patterns on azo dye transformation by CotA-laccase. Faculdade de Medicina Veterinária, Universidade de Lisboa

 

Catarina Pimenta, Feb-Nov 07

Biodegradation of Sudan Orange G by the bacterium Pseudomonas putida. Co-supervision: Luciana Pereira. Escola Superior Agrária de Castelo Branco

 

Rui Coelho, April 06-Dec 06

Expression, Purification and characterization of a “multicopper oxidase” from the hyperthermophilic archaeon Pyrobaculum aerophilum. Bachelor Biotechnol Eng, Universidade Lusófona de Humanidades e Tecnologias

 

Diana Mateus, April -Dec 05

Biodegradation of synthetic dyes from textile industries by Pseudomonas putida MET94. Bachelor Biotechnol Eng, Universidade Lusófona de Humanidades e Tecnologias

 

André T. Fernandes, Oct 04-Jul 05

Cloning, expression, purification and characterization of a laccase from the hyperthermophilic bacterium Aquifex aeolicus. Bachelor Biotechnol Eng, Universidade Lusófona de Humanidades e Tecnologias


Erasmus students

Lorenzo Benardo, March-Sep 2017 (7 months)

Multienzymatic systems to develop new industrial value chains from organic wastes. Universitá degli Studi di Torino, Italy

 

Beata Rekosz, Aug-Oct 15 (3 months)

Isolation and identification of new bacterial lignin-degrading strains. Technical University of Lodz, Poland  

 

Kamil Trzebinski, July-Sep14 (3 months)

Cloning and heterologous expression of a glutathione S-transferase (GST) from Myxococcus stipitatus. Technical University of Lodz, Poland                       

 

Marcin Jarzebski, July-Sep14  (3 months)

Immobilization and spectroscopic studies of glyoxal oxidases. Co-supervisor: Smilja Todorovic. Technical University of Lodz, Poland

 

Lina Jobuškaitė, Jun-Aug 12 (3 months)

Improvement of the heterologous expression of the multicopper oxidase McoP from Pyrobaculum aerophilum. Vilnius Gediminas Technical University Institute of Biochemistry, Vilnius, Lithuania

 

Marek Voišnis, Jun-Aug 12 (3 months)

Comparison of enzymatic activities of the recombinant azoreductase PpAzoR from Pseudomonas putida MET94 using NADPH or NADH. Vilnius Gediminas Technical University Institute of Biochemistry, Vilnius, Lithuania

 

Sabrina Föge , Aug 08-Feb 09 (6 months)

Directed Evolution of bacterial laccases. Technische Universitat Hamburg-Harburg, Germany

 

Amanda Fillat Latorre, Feb - July 06 (6 months)

Expression, purification and characterization of the “multicopper oxidase” from Campylobacter jejuni. Univ Catalonia, Barcelona, Spain


Summer training

João Costa (Biochemistry FCUL), July 2019

Directed evolution techniques; Studies with AsP2Ox, a bacterial Pyranose 2-oxidase

 

Catalina Cepeleaga (Cell and Mol Biology, FCT NOVA), July 2019

Directed evolution techniques; Studies with McoP from Pyrobaculum aerophilum

 

Rita Marques Pinto (Biological Sciences, Plymouth Univ), 23-27 July, 2018

Biodegradation and valorisation of dye-containing wastewaters using immobilized cells of recombinant Escherichia coli overproducing an azoreductase and a laccase. Summer Science @ ITQB-NOVA

 

Ana Buga (Biochemistry, FCT-UNL), 15 June-15 July, 2018

Studies with AsP2Ox, a bacterial Pyranose 2-oxidase

 

João Zagalo Pereira (Biology, FC-UL), July 24-28, 2017

Comparative analysis of the catalytic specificity for phenolics of two DyP-peroxidases. Summer Science @ ITQB-NOVA

 

José Bento (Biology, FCT-UNL), July 17-28, 2017

Comparative analysis of the catalytic specificity for phenolics of bacterial laccases

 

Pilar Portela (FCT-UNL) & Diana Marques (Univ Aveiro), July 25-29, 2016

Summer Science @ ITQB-NOVA

 

Mafalda Duque (IST UL), July-Aug 15

Directed evolution of Arthrobacter siccitolerans galactose oxidase for improved solublity.

 

Mónica Pereira (IST UL), July-Aug 15

Immobilization of PpDyP for generation of biosensors. Co-supervisor: Smilja Todorovic.

 

Lídia Jesus (IST UL), July-Aug 15

Cloning PpAzoR and PpDyP in pET-Duet.

 

Ana Margarida Alves (IST UL), July-Sep 13

Isolation of new bacterial strains of lignin degraders.

 

Inês Almeida (IST UL), July-Sep 13

Isolation of new bacterial strains for biosurfactants production.

 

Tiago Pinto (IST UL), July-Dec 12

Crystallization of bacterial DyP-peroxidases.

 

André Belo (IST UL), July-Aug 12

Screening ionic liquids for lignin solubilization.

 

Marcelo Ferreira, (IST UL) July-Sep 12

Development of a colorimetric screening method for lignin degradation

 

Alessa Silva, (FCUL, UL) Aug-Sep10

Effect of organic solvents and ionic liquids in the activity of CotA.

 

Hugo Sant’Ana Pereira (UCL, UK), Sep09

Directed evolution CotA-laccase

 


High-School Students

Rafael Claudino, Jun-Jul 17 (2 months)

Superprodução e purificação de enzimas recombinantes. Escola Secundária do Pinhal Novo, Curso Profissional de Técnico de Análises Laboratorais Nível III, 11º grade.

 

Soraia Barata, May-Jul 13 (3 months)

Crescimento de microrganismos e produção de proteínas heterólogas. Training period (Prova de Apditão Profissional) “Curso Profissional de Técnico de Análises Laboratorais Nível III” – 12º grade. Escola Secundária de Mem Martins

 

Sofia Almeida May-Nov 12 (6 months)

Superprodução e purificação de enzimas recombinantes. Training period (Prova de Apditão Profissional) “Curso Profissional de Técnico de Análises Laboratorais Nível III” – 12º grade. Escola Secundária de Mem Martins.

 

Tiago Pereira Oct-Mar 11 (6 months)

Superprodução e purificação de enzimas recombinantes. Training period (Prova de Apditão Profissional) “Curso Profissional de Técnico de Análises Laboratorais Nível III” – 12º grade. Escola Secundária de Mem Martins.

 


Visiting Researchers

 

Magalí Scoccozza, May-Nov (6 months)

PhD student, INQUIMAE, Buenos Aires, Argentina

 

Alain Puente Santiago, Oct-Nov 17 (2 months)

Post-doc in Physical Chemistry, Univ Cordoba, Spain

 

Ana Isabel Vicente, Sep-Dec 15 (3 months)

PhD student, CSIC, Department of Biocatalysis, Madrid, Spain

 

Ana Paula Tavares, Dec 13

Faculdade de Engenharia, Univ Porto, Portugal

 

Alba Blánquez, Apr-Jul 13 (3 months)

PhD student, Universidad Alcalá, Madrid, Spain

 

Yasmina Mekmouche, Nov-Dec 12 (2 months)

Researcher,  Université Marseille, France

 

Weeranuch Lang, Sep-Nov 12 (3 months)

Post-Doc, Kokkaido University, Sapporo, Japan

 

Maria Isabel Guijon, Feb-Jul 11 (6 months)

Lecturer, Universidad de Cáceres, Spain

 

Debjani Dutta, Nov-Dec 09 (1 month)

Biotechnology Department, National Institute of Technology, Durgapur, India

 

Nuria Gómez Santos, Oct-Dec06, Sep-Dec08 (6 months)

PhD student, Dep Microbiologia, Univ Granada, Spain


Resident artist

 

Marta de Menezes  March 06-April 08

Ectopia, Instituto Gulbenkian da Ciência, Oeiras, Portugal

 

 

 

 

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