CP Ricardo

Name: Cândido Pereira Pinto Ricardo
Place of birth: Vila Nova de S. Bento (Serpa)
Date of birth: 18.08.1936
Nationality: Portuguese
Academic degrees: "Engenheiro Agrónomo" (Instituto Superior de Agronomia; Universidade Técnica de Lisboa, 1962); Ph.D in Plant Biochemistry (Cambridge University, UK, 1968); "Agregação" (Instituto Superior de Agronomia; Universidade Técnica de Lisboa, 1984)

Professional positions: Lecturer (1969-1993), Full Professor (1993-2006) of Plant Physiology at "Departamento de Botânica e Engenharia Biológica" (Instituto Superior de Agronomia; Universidade Técnica de Lisboa); 2006-date, Emeritus Professor (Instituto Superior de Agronomia; Universidade Técnica de Lisboa); 1989-date, Head of the Plant Biochemistry Laboratory at "Instituto de Tecnologia Química e Biológica" (Universidade Nova de Lisboa)
Institutional address: Instituto de Tecnologia Química e Biológica; Apartado 127; 2781-901 Oeiras
Telephone: +351 21 4469654
Fax: +351 21 4433644
E.mail: ricardo@itqb.unl.pt

Main scientific area of research: Plant Biochemistry
Scientific interests: Plant responses to environmental stress (drought, temperature, minerals); proteins/proteomics; transcriptomics; carbohydrate metabolism
Main Publications:

Books
Ricardo, C.P.P. and Teixeira, A.R.N. (1976) "Enzimas". Didáctica Editora, Lisboa (160 pp.).
Ricardo, C.P.P. and Teixeira, A.R.N. (1977) "Moléculas Biológicas; Estrutura e Propriedades". Didáctica Editora, Lisboa (280 pp.).
Teixeira, A.R.N. and Ricardo, C.P.P. (1983) "Mitocôndrio e Metabolismo Respiratório". Didáctica Editora, Lisboa (260 pp.).
Teixeira, A.R.N. and Ricardo, C.P.P. (1983) "Fotossíntese". Didáctica Editora, Lisboa (344 pp.).
Ricardo, C.P.P. and Teixeira, A.R.N. (1984) "Moléculas Biológicas; Estrutura e Propriedades". (2ª edição) Didáctica Editora, Lisboa (301 pp.).

Translation
Amâncio, S., Teixeira, A.R.N., Ricardo, C.P.P. and Santos, A. (1983) "Introdução à Fisiologia Vegetal" (Introduction to Plant Physiology, 2nd edition, by Meyer, S., Anderson, D.B., Bohning, R.H. and Fratianne, D.G.D. van Nostrand, New York, (1973). Fundação Gulbenkian, Lisbon (710 pp.).

Research Articles
Ricardo, C.P.P. and ap Rees, T. (1970) Invertase activity during the development of carrot roots. Phytochemistry, 9, 239-247.
Ricardo, C.P.P. and ap Rees, T. (1972) Activities of key enzymes of carbohydrate oxidation in disks of carrot storage tissue. Phytochemistry, 11, 623-626.
Ricardo, C.P.P., ap Rees, T. and Fuller, W.A. (1972) Effects of sugars on invertase activity of carrot cells. Phytochemistry, 11, 2435-2436.
Ricardo, C.P.P. and Sovia, D. (1974) Development of tuberous roots and sugar accumulation as related to invertase activity and mineral nutrition. Planta (Berl.), 118, 43-55.
Ricardo, C.P.P. (1974) Alkaline *-fructofuranosidases of tuberous plant roots: possible physiological function. Planta (Berl.), 118, 333-343.
Ricardo, C.P.P. (1976) Effects of sugars, gibberellic acid and kinetin on acid invertase of developing carrot roots. Phytochemistry, 15, 615-617.
Ricardo, C.P.P. and Passarinho, J.A.P. (1983) Cation effects on invertase development and leakiness of carrot slices. Phytochemistry, 22, 1701-1706.
Casares, A., Melo, E.M.P.F., Ferraz, J.F.P. and Ricardo, C.P.P. (1986) Differences in ability of Phytophthora cambivora and P. cinnamomi to dephenolize lignin. Trans. Br. Mycol. Soc., 87, 229-235.
Jorge-Silva, M.L., Rodrigues, M.L., Ferraz, J.F.P. and Ricardo, C.P.P. (1989) Effect of water availability on growth of Fusarium oxyporium f. sp. melonis and on host-parasite interactions. Mycological Research, 72, 157-161.
Moreira, M.A., Veloso, M.M., Ricardo, C.P.P. and Duque-Magalhães, M.C (1990) Changes of wheat peptido-hydrolases during differentiation and dedifferentation. Plant Science, 66, 51-57.
Ricardo, C.P.P. and Santos, H. (1990) Application of 31P NMR to monitor phosphorus compounds and their changes during germination of legume seeds. J. Exp. Botany, 41, 79-87.
Jackson, P. and Ricardo, C.P.P. (1992) Cytochrome c aided resolution of Lupinus albus isoperoxidases in a cathodal polyacrylamide gel electrophoresis system. Analytical Biochemistry, 200, 36-41.
Silva, M.P. and Ricardo, C.P.P. (1992) *-Fructosidases and in vitro dedifferentation-redifferentation of carrot cells. Phytochemistry, 31, 1507-1511.
Duarte, I.C., Ricardo, C.P.P. and Duque-Magalhães, M.C. (1993) Peptide hydro¬lases in cotyledons of germinating lupin. Phytochemistry, 33, 35-40.
Vassilevskaia, T.D., Ricardo, C.P. and Rodrigues-Pousada, C. (1993) Molecular cloning and sequencing analysis of a *-tubulin gene from Lupinus albus. Plant Molec. Biology 22, 715-718.
Jacinto, A., Neves, A.M., Vassilevskaia, T.D., Ricardo, C.P.P. and Rodrigues-Pousada, C. (1994) Cloning and characterization of two ubiquitin:: 79 - amino-acid extension protein-encoding fusion genes from Lupinus albus. Gene, 139, 201-205.
Costa, J. and Ricardo C.P.P. (1994) Changes in the composition of ConA-binding glycopeptides from Lupinus albus root membranes induced by nodulation and callus formation. Plant Physiol. Biochem., 32, 295-302.
Jackson, P. and Ricardo, C.P.P. (1994). An examination of the peroxidases from Lupinus albus L. hypocotyls. Planta, 194, 311-317.
Costa, J. and Ricardo, C.P.P. (1994) Tunicamycin and swainsonine stimulate Lupinus albus L. root growth in vitro. Plant Science, 101, 137-142.
Borges, M.L., Matos, O.C., Pais, I., Melo, J.S., Ricardo, C.P.P., Maçanita, A. and Becker, R.S. (1995) Evaluation of a broad variety of coumarins, chromones, their furohomologs and thione analogs as photoxins activated by UVA and visible light. Pesticide Science, 44, 155-162.
Ferreira, R.M.B., Ramos, P.C.R., Franco, E., Ricardo, C.P.P. and Teixeira, A.R.N. (1995) Changes in ubiquitin and ubiquitin-protein conjugates during seed formation and germination. J. Exp. Botany, 46, 211-219.
Gouvêa, C.M.C.P., Ricardo, C.P.P. and Oliveira, A.R. (1995) Accumulation of a host microsomal fraction protein in virus-infected cucumber and tomato. Plant Physiol. Biochem., 33, 453-461.
Ramos, P.C.R., Cordeiro, A.C.F., Ferreira, R.M.B., Ricardo, C.P.P. and Teixeira, A.R.N. (1995) The presence of ubiquitin-protein conjugates in plant chloroplast lysates is due to cytosolic contamination. Aust. J. Plant Physiol., 22, 893-901.
Pinto, M.P. and Ricardo, C.P.P. (1995) Lupinus albus L. pathogenesis - related proteins that show similarity to PR-10 proteins. Plant Physiol., 109, 1345-1351.
Regalado, A.P. and Ricardo, C.P.P. (1996) Study of the intercellular fluid of healthy Lupinus albus organs: presence of a chitinase and a thaumatin-like protein. Plant Physiol., 110, 227-232.
Costa, J., Ashford, D.A. and Ricardo, C.P.P. (1996) Characterisation of a developmentally related polypeptide with glutelin solubility characteristics from Lupinus albus L. Planta, 198, 221-229.
Duarte, I., Ricardo, C.P.P. and Duque-Magalhães, M.C. (1996) Proteolysis in the quiescent seed. Physiol. Plantarum, 96, 519-525.
Ramos, P.C.R., Ferreira, R.M.B. and Ricardo, C.P.P. (1996) Synthesis of 125I-ubiquitin conjugates in extracts of Lemna minor. J. Exp. Bot., 47, 569-575.
Vassilevskaia, T.D., Bekman, E., Jackson, P., Ricardo, C.P.P. and Rodrigues-Pousada, C. (1996) Developmental expression and regulation by light of two closely related -tubulin genes in Lupinus albus. Plant Molec. Biol., 32, 1185-1189.
Costa, M.M.R., Costa, J. and Ricardo, C.P.P. (1997) A Lupinus albus root glycoprotein homologous to the polygalacturonase inhibitor proteins. Physiol. Plantarum, 99, 263-270.
Jackson, P. and Ricardo, C.P.P. (1998) The changing peroxidase polymorphism in Lupinus albus during vegetative development. Aust. J. Plant Physiol, 25, 261-269.
Jackson, P., Paulo, S., Brownleader, M.D., Freire, P.O., Ricardo, C.P.P.(1999) An extensin peroxidase is associated with white-light inhibition of lupin (Lupinus albus) hypocotyl growth. Australian J. Plant Physiology, 26, 29-36.
Matos, O.C., Baeta, J., Silva, M.J. and Ricardo, C.P.P. (1999) Sensitivity of pathogenic fungi to Chelidonium majus L. extracts. J. Ethno-Pharmac., 66, 151-158.
Regalado, A.P., Pinheiro, C., Vidal, S., Chaves, I., Ricardo, C.P.P. and Rodrigues-Pousada, C. (2000) The Lupinus albus class III chitinase gene, IF3, is constitutively expressed in vegetative organs and developing seeds. Planta, 210, 543-550.
Passarinho, J.A., Rodrigues, M.L., Osório, M.L., Ricardo, C.P.P. and Chaves, M.M. (2000) Physiological responses of Lupinus mutabilis to phosphorus nutrition and season of growth. J. Plant. Nutrition, 23, 487-505.
Bekman, E.P., Saibo, N.J.M., Di Cataldo, A., Regalado, A.P., Ricardo, C.P. and Rodrigues-Pousada, C. (2000) Differential expression of four genes encoding ACC synthase in Lupinus albus during germination, growth and in response to IAA, Li+, and wounding. Planta, 211, 663-672.
Pinheiro, C., Chaves, M.M. and Ricardo, C.P. (2001) Alterations in carbon and nitrogen metabolism induced by water deficit in the stems and leaves of Lupinus albus L. J. Exp. Botany, 52, 1063-1070.
Jackson, P., Galinha, C., Pereira, C., Fortunato, A., Soares, N., Amâncio, S. and Ricardo, C.P. (2001) Rapid deposition of extensin during the elicitation of grapevine callus cultures is specifically catalysed by a 40 kDa peroxidase. Plant Physiol., 127, 1065-1076.
Chaves, M.M., Pereira, J.S., Maroco, J., Rodrigues, M.L., Ricardo, C.P.P., Osório, M.L, Carvalho, I., Faria, T. and Pinheiro, C. (2002) How do plants cope with water stress in the field: photosynthesis and growth. Annals Botany, 89, 907-916.
Chaves, I., Regalado, A.P., Chen, M., Ricardo, C.P. and Showalter, A.M. (2002) Programmed cell death induced by (*-D-galactosyl)3 Yariv reagent in Nicotiana tabacum BY-2 suspension-cultured cells. Physiol. Plantarum, 116, 548-553.
Price, N.J., Pinheiro, C., Soares, C.M., Ashford, D.A., Ricardo, C.P. and Jackson, P.A. (2003) A biochemical and molecular characterization of LEP1, an extensin peroxidase from lupin. J. Biol. Chemistry, 278, 41389-41399.
Vaz, A.C., Pinheiro, C., Martins, J.M.N. and Ricardo, C.P.P. (2004) Cultivar discrimination of Portuguese Lupinus albus by seed protein electrophoresis: the importance of considering “glutelins” and glycoproteins. Field Crops Research, 87, 23-34.
Carvalho, I.S., Ricardo, C.P. and Chaves, M.. (2004) Quality and distribution of assimilates within the whole plant of lupines (L. albus and L. mutabilis ) influenced by water stress. J. Agronomy and Crop Science, 190, 205-210.
Vatulescu, A.D., Fortunato, A.S., Sá, M.C., Amâncio, S., Ricardo, C.P.P. and Jackson, P.A. (2004) Cloning and characterization of a basic IAA oxidase associated with root induction in Vitis vinifera. Plant Physiol. Biochem., 42, 609-615.
Pinheiro, C., Passarinho, J.A. and Ricardo, C.P. (2004) Effect of drought and rewatering on the metabolism of Lupinus albus organs. J. Plant Physiology, 161, 1203-1210.
Carvalho, I.S., Chaves, M. and Ricardo, C.P. (2005) Influence of water stress on the chemical composition of seeds of two lupins (Lupinus albus and Lupinus mutabilis ). J. Agronomy and Crop Science, 191, 95-98.
Pinto, M.P., Ribeiro, A., Regalado, A.P., Rodrigues-Pousada, C. and Ricardo, C.P.P. (2005) Expression of Lupinus albus PR-10 proteins during root and leaf development. Biol. Plantarum, 49, 187-193.
Carvalho, L.C., Esquivel, M.G., Martins, I, Ricardo, C.P. and Amâncio, S. (2005) Monitoring the stability of Rubisco in micropropagated grapevine (Vitis vinifera L.) by two dimensional electrophoresis. J. Plant Physiology, 162, 365-374.
Pinheiro, C., Kehr, J. and Ricardo, C.P. (2005) Effect of water stress on lupin stem proteins analyzed by two-dimensional gel electrophoresis. Planta, 221, 716-728.
Batista, R.,Nunes, B., Carmo, M., Cardoso, C., São-José, H. Almeida, A.B., Manique, A., Bento, L., Ricardo, C.P. and Oliveira, M.M. (2005) Lack of detectable allergenicity of transgenic maize and soya samples. J. Allergy Clin. Immunol., 116, 403-410.
Pinheiro, C., Rodrigues, A.P., Carvalho, I.S., Chaves, M.M. and Ricardo, C.P. (2005) Sugar metabolism in developing lupin seeds affected by a short-term water deficit. J. Exp. Botany, 56, 2705-2712.
Baldé, J.A., Francisco, R., Queiroz, A., Regalado, A.P., Ricardo, C.P. and Veloso, M.M. (2006) Immunolocalization of a class III chitinase in two muskmelon cultivars reacting differently to Fusarium oxysporum f. sp. melonis. J. Plant Physiology, 163, 19-25.
Alves, M., Francisco, R., Martins, I. and Ricardo, C.P.P. (2006) Analysis of Lupinus albus leaf apoplastic proteins in response to boron deficiency. Plant and Soil, 279, 1-11.
Passarinho, J.A.P., Lamosa, P., Baeta, J.P., Santos, H. and Ricardo, C.P.P. (2006) Annual changes in the concentration of minerals and organic compounds of Quercus suber L. leaves. Physiol. Plantarum, 127, 100-110.
Batista, R., Martins, I., Jenö, P, Ricardo, C.P. and Oliveira, M.M. (2007) A proteomic study to identify soya allergens – the human response to transgenic vs. non transgenic soya samples. Intern. Archives Allergy Immunol., 144, 29-38.
Soares, N.C., Francisco, R., Ricardo, C.P. and Jackson, P.A. (2007) Proteomics of ionically bound and soluble extracellular proteins in Medicago truncatula leaves. Proteomics, 7, 2070-2082.
Fortunato, A., Santos, P., Graça, I., Gouveia, M.M., Martins, S.M., Ricardo, C.P.P., Pawlowski, K. and Ribeiro, A. (2007) Isolation and characterization of cgchi3, a nodule-specific gene from Casuarina glauca encoding a class III chitinase. Physiol. Plantarum, 130, 418-426.
Pinheiro, C., Carvalho, M.H.C., Bartels, D., Ricardo, C.P.P and Chaves, M.M. (2008) Dehydrins in Lupinus albus: pattern of protein accumulation in response to drought. Functional Plant Biology, 35, 85-91.
Antonio, C., Pinheiro, C., Chaves, M.M., Ricardo, C.P., Ortuño, M.F. and Thomas-Oates, J. (2008) Analysis of carbohydrates in Lupinus albus stems on imposition of water deficit, using porous graphitic carbon liquid chromatography-electrospray ionisation mass spectrometry. J. Chromatography A, 1187, 111-118.
Carrilho, D.M., Duarte, I.C., Francisco, R., Ricardo, C.P.P. and Duque-Magalhães, M.C. (2009) Discovery of novel plant peptides as strong inhibitors of metalloproteinases. Protein and Peptide Letters, 16, 543-551.
Guerra-Guimarães, L., Silva, M.C., Struck, C., Loureiro, A., Nicole, M., Rodrigues Jr., C.J. and Ricardo, C.P.P. (2009) Chitinases of Coffea arabica genotypes resistant to orange rust Hemileia vastatrix. Biologia Plantarum, 53, 702-706.
Chaves, I., Pinheiro, C., Paiva, J.A.P., Planchon, S., Sergeant, K., Renaut, J., Graça, J.A., Costa, G., Coelho, A.V., Ricardo, C.P.P. (2009) Proteomic evaluation of wound-healing processes in potato (Solanum tuberosum L.) tuber tissue. Proteomics, 9, 4154-4175.
Sergeant, K., Pinheiro, C., Hausman, J.-F., Ricardo, C. P. and Renaut, J. (2009) Taking advantage of non-specific trypsin cleavages for the identification of seed storage proteins in cereals. Journal of Proteome Research, 8, 3182-3190.
Soares, N., Francisco, R., Vielba, J., Ricardo, C. and Jackson, P. (2009) Associating wound-related changes in the apoplast proteome of Medicago with early steps in the ROS signal-transduction pathway. Journal of Proteome Research, 8, 2298-2309.
Santos, P., Fortunato, A., Graça, I., Martins, S.M., Gouveia, M.M., Auguy, F., Bogusz, D., Ricardo, C.P.P., Pawlowski, K. and Ribeiro, A. (2010) Characterization of four defense-related genes up-regulared in root nodules of Casuarina glauca. Symbiosis, 50, 27-35.
Alves, M., Chaves, I., Carrilho, D., Veloso, M., Ricardo, C.P. (2010) Detection of novel trypsin inhibitors in the cotyledons of Phaseolus vulgaris seeds. Journal of Plant Physiology, 167, 848-854.
Felisberto-Rodrigues, C., Ribeiro, I.C., Veloso, M., Ricardo, C.P. and Pinheiro, C. (2010) Germination under aseptic conditions of different ecotypes of wild beet (Beta vulgaris L. ssp. maritima). Seed Science Technology, 38, 517-521.
Pinheiro, C., Baeta, J.P., Pereira, A.M., Domingues, H. and Ricardo, C.P. (2010) Diversity of seed mineral accumulation of Phaseolus vulgaris L. germplasm. Journal of Food Composition and Analysis, 23, 319-325.
Matos, M.C., Campos, P.S., Passarinho, J.A., Semedo, J.N., Marques, N.M., Ramalho, J.C. and Ricardo, C.P. (2010) Drought effect on photosynthetic activity, osmolyte accumulation and membrane integrity of two Cicer arietinum genotypes. Photosynthetica, 48, 303-312.
Chaves, I., Alves, M., Carrilho, D., Duque-Magalhães, M.C., Ricardo, C.P. and Regalado, A.P. (2011) HSP60 and HSP70 resist proteolysis during PCD-induced in tobacco BY-2 cells by (*-D-galactosyl)3 Yariv reagent. Biologia Plantarum, 55, 153-158.
Ricardo, C.P.P., Martins, I., Francisco, R., Sergeant, K., Campos, A., Renaut, J. and Fevereiro, P. (2011) Proteins associated with cork formation in Quercus suber L. stem tissues. Journal of Proteomics, 74, 1266-1278..
Alves, M., Moes, S., Jenö, P., Pinheiro, C., Passarinho, J. and Ricardo, C.P. (2011) The analysis of Lupinus albus root proteome revealed cytoskeleton altered features due to long-term boron deficiency. Journal of Proteomics, 74, 1351-1363.
Chaves, I., Passarinho, J.A., Capitão, C., Chaves, M.M., Fevereiro, P. and Ricardo, C.P. (2011) Temperature stress effects in Quercus suber leaf metabolism. J. Plant Physiology, 168, 1729-1734.
Lourenço, T., Saibo, N., Batista, R., Ricardo, C.P. and Oliveira, M.M. (2011) Inducible and constitutive expression of HvCBF4 in rice leads to differential gene expression and drought tolerance. Biologia Plantarum, 55, 653-663.
Pinheiro, C., Antonio, C., Ortuño, M.F., Dobrev, P.I., Hartung, W., Thomas-Oates, J., Ricardo, C.P., Vankova, R., Chaves, M.M. and Wilson, J.C. (2011) Initial water deficit effects on Lupinus albus photosynthetic performance, carbon metabolism and hormonal balance: metabolic reorganization prior to early stress responses. J. Exp. Botany, 62, 4965-4974.
Marum, L., Miguel, A., Ricardo, C.P. and Miguel, C. (2012) Reference gene selection for quantitative real-time PCR normalization in Quercus suber. PLoS One, 7(4), e35113.
Alves, M., Chicau, P., Matias, H., Passarinho, J., Pinheiro, C. and Ricardo, C.P. (2011) Metabolic analysis revealed altered amino acid profiles in Lupinus albus organs due to boron deficiency. Physiol Plantarum, 142, 224-232.
Almeida, A.M., Salvado, J., Santos, R., Duque, S., Francisco, R., Tomé, D., Ricardo, C.P., Coelho, A,V. and Fevereiro, P. (2012) A proteomics study of the induction of somatic embryogenesis in Medicago truncatula using 2DE and MALDI-TOF/TOF. Physiol. Plantarum (in press).