. Cell disruption
. Ultra-centrifugation (preparation of membrane and soluble extract)
. Chromatographic steps
. Purity assessment

Characterization

. Cofactor analysis – extraction, identification and quantification
. Quaternary structure analysis
. Redox properties followed by potentiometry with visible and/or EPR spectroscopy
. Steady-state kinetics followed by UV-visible spectroscopy

Spectroscopy

. UV-visible
. Electron Paramagnetic Resonance

Polarography & Amperometry – Clark-type Electrodes

. oxygen polarography
. nitric oxide amperometry