Basic course on NMR spectroscopy

3rd CERMAX practical course on basic NMR

Program

14th of June

  9.00 – Introduction to NMR spectroscopy (the phenomenon, basic properties of the signal).
10.00 – Instrumental aspects of the spectrometer /Rules for spectrometer use.
10.45 – Break
11.15 – Introduction to TopSpin software.
13.00 – Lunch break
14.30 – Practical aspects of acquisition and processing (acquisition parameters, pulse programs, 90º pulse and T1 determination). 
15.30 – Break
16.00 – Spectrometer Guided tour and sample preparation

15th of June
10.00 – The Nuclear Overhauser Effect 
10.30 – Break
11.00 – Practical session I
               Acquisition (1D, presat, p90, APT)
               Processing and analyzing 1D
13.00 – Lunch break
14.30 – Practical sessions I (cont)

16th of June
  9.00 –Introduction to 2D NMR spectroscopy (Homonuclear correlation) 
10.00 – INEPT, HSQC and HMBC
11.00 – Break
11.30 – DOSY
12.15 – Protein structure
13.00 – Lunch break
14.30 – Practical session II
               Acquisition (COSY, HSQC)
               Processing and analyzing 2D

17th of June
10.00 – Assignment strategies in small molecules (tutorial and exercises)
11.00 – Break
11.30 – Assignment strategies (cont)
13.00 – Lunch break
14.30 – Practical session III
               Acquisition exercises

Faculty: Helena Matias
              Manolis Matzapetakis 
              Patrick Groves
              Pedro Lamosa