[Workshop] IMpaCT - Data Processing Workshop on Cryo-EM applied to macromolecular complexes
| When |
17 Sep, 2019 09:00 AM
to
20 Sep, 2019 06:30 PM |
|---|---|
| Where | Room 3.02 |
| Contact Name | Célia Romão |
| Add event to your calendar |
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IMpaCT - Data Processing Workshop on Cryo-EM applied to macromolecular complexes: single particle analysis and atomic modellinga
Tuesday, September 17
18:30 Welcome drink and Registration
Wednesday, September 18
9:00 Introduction to the course
9:15 Introduction to 3D EM
10:00 Basic background: sampling, Fourier Transform, central section, sampling, CTF
11:00 Coffee Break
11:30 Movie alignment, quality estimation, CTF determination
13:00 Lunch
14:00 Particle picking and particle screening
16:00 Tea Break
16:30 2D Classification
18:00 End of day
Thursday, September 19
9:00 Initial volume, 3D alignment and reconstruction
11:00 Coffee Break
11:30 3D Classification
13:00 Lunch
14:00 Resolution and quality assessment
16:00 Tea Break
16:30 Structure prediction by sequence homology
18:00 End of day
19:00 Workshop Dinner
Friday, September 20
9:00 Rigid fitting
11:00 Coffee Break
11:30 Flexible fitting
13:00 Lunch
14:00 Structure validation and comparison
16:00 Tea Break
16:30 Building the unit cell and the whole macromolecule
18:00 End of course
more info at IMpaCT website
