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[SCAN] Exopolysaccharide structure by NMR - from structure to genes to structure

Pedro Lamosa

When 07 Apr, 2021 from
12:00 pm to 01:00 pm
Contact Name Rita Abranches
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Speaker: Pedro Lamosa

Title: Exopolysaccharide structure by NMR - from structure to genes to structure

Abstract: Exopolysaccharides (EPSs) are of paramount importance in texture and taste of fermented foods, particularly in the dairy industry in the use of Lactic Acid Bacteria (LAB) for the production of cheeses and yogurts. The organoleptic properties of EPSs are derived from its structures (monomer composition, degree of branching and glycoside linkage patterns). Even closely related strains may have very different EPS structures.
Experimental structure elucidation of EPS is time-consuming and expensive. So, the possibility to derive EPS structure from genomic information would be highly appealing. Here, we have investigated the structures of EPSs from 12 strains of S. thermophilus whose genome information is available, that revealed 6 novel repeat unit structures not previously observed, and derived a procedure to putatively assigned glycosyltransferases to one or more monomers in the repeat units. This is a first step towards predicting EPS structures from genomic information.

 

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