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[SCAN] Metalloenzymes - from biophysics to biosensors

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Célia Silveira

When 19 Jun, 2024 from
12:00 pm to 01:00 pm
Where ITQB NOVA Auditorium
Contact Name Sandra Viegas
Contact Email
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Title: Metalloenzymes - from biophysics to biosensors

Speaker: Célia Silveira 

Affiliation: Raman BioSpectroscopy Lab, ITQB NOVA

Abstract: The use of metalloenzymes as biocatalysts in biosensors, biofuel cells, and bioreactors is at the forefront of current biotechnology research. We investigate the structural and electrocatalytic properties of a number of bacterial redox enzymes as immobilized biocatalysts for use in electrochemical biosensors and for the electrocatalytic degradation of environmental pollutants. Using resonance Raman (RR) and surface-enhanced RR (SERR) spectroscopy, we can identify promising enzyme candidates for biotechnology applications without relying on traditional trial-and-error approaches. Here, we demonstrate how this strategy enabled the construction of an efficient biosensor for H2O2 detection, based on dye-decolorizing peroxidases (DyPs), which outperforms devices based on commonly used biocatalysts, like horseradish peroxidase (HRP).

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