[Seminar] Cytoplasmic live-cell biopsies and transfection of single-cells with FluidFM

Title: Cytoplasmic live-cell biopsies and transfection of single-cells with FluidFM

Speaker: Tamás Gerecsei

From: Cytosurge

Abstract: Fluidic force microscopy or FluidFM is a biophysical technique for manipulating single-cells using a force-controlled cantilever with a microfluidic channel inside of it. This innovative approach enables the extraction of a part of the cytoplasm from individual living cells while preserving their viability.

These cytoplasmic biopsies can be used for subsequent highly-sensitive, low-input RNA-seq analysis to characterize single-cells multiple times throughout their lifetime.

Moreover, the FluidFM Nanosyringes extend their utility by facilitating the targeted introduction of various molecular components into cells, including RNA, DNA, proteins and even molecular complexes such as CRISPR/Cas9 RNPs.

This functionality streamlines the transfection processes for plasmids, and transcription factors, as well as enabling entire cell line engineering workflows.

By exploring the capabilities of FluidFM in this seminar, we seek to uncover its potential implications for advancing the comprehension of intricate cellular processes, thus fostering new dimensions in cellular analysis and molecular investigation.