[SCAN] FTIR difference spectroscopy: a hand lens on metalloproteins structure-function relationship
Lidia Zuccarello
| When |
08 Jul, 2020
from
12:00 pm to 01:00 pm |
|---|---|
| Where | Zoom Webinar |
| Contact Name | Rita Abranches |
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Speaker: Lidia Zuccarello
Affiliation: Smilja Todorovic Lab
Title: FTIR difference spectroscopy: a hand lens on metalloproteins structure-function relationship
Abstract: Metalloproteins play a central role in various metabolic pathways of living organisms, covering a large variety of functions by the diversity of the metallic cofactor, i.e. metal cations, coordination spheres, and specific structures of the metal centres. Although the relationship between structural properties of cofactor and its environment and function of protein is widely recognised, a deep understanding of molecular mechanism at the active site is often missing.
Vibrational spectroscopy allows the investigation of structural properties of materials and when properly adapted is a powerful tool in the study of mechanistic events at the basis of protein function.
This SCAN aims to give an overview on the Fourier Transform Infrared (FTIR) difference spectroscopy. A multiplicity of setups and the possibility of coupling FTIR spectroscopy with electrochemistry or photochemistry, give complementary hints in the structure-function relationship study. Examples from metalloproteins of the thermophilic cyanobacterium Thermosynechoccocus elongatus will be shown, with particular emphasis on plant-type [2Fe-2S] ferredoxins, and [Mn4CaO5] Oxygen Evolving Complex (OEC), in Photosystem II.
Zoom webinar - ITQB NOVA Virtual auditorium
