[Seminar] High resolution, or low resolution: Is this really the question?
Jaime Martin-Benito Romero
| When |
21 Feb, 2022
from
03:30 pm to 04:30 pm |
|---|---|
| Where | ITQB NOVA Auditorium |
| Contact Name | Célia Romão |
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Title: High resolution, or low resolution: Is this really the question?
Speaker: Jaime Martin-Benito Romero,
Lab Head of the "Ultrastructure of viruses and molecular aggregates" Group, CSIC, CNB, Madrid SPAIN
Abstract: In recent years electron cryomicroscopy has undergone a real revolution driven by the development of direct electron detectors, the automation of microscopes and a huge development in image processing software. This, which has been termed the "resolution revolution", has made possible to obtain protein structures at quasi-atomic resolution (between 2 and 5 Å) almost routinely, enabling very detailed studies of biological systems. Nevertheless, there are factors such as the extreme flexibility or conformational variability of some macromolecular complexes, which in many cases still prevent achieving high resolution. However, even in these cases, electron microscopy can provide us with information of great value in answering important biological questions. In this seminar we will see two examples of both scenarios, the determination of the structure of a membrane protein at high resolution, and the determination of the transcription mechanism of influenza virus ribonucleoproteins using low resolution studies.
