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Methods most commonly used at GPlantS

  • DNA and RNA extraction
  • PCR and Real-Time qPCR)
  • RNA-seq analysis (e.g. read mapping, de novo assembly, differential expression)
  • Yeast-one hybrid and Yeast-two hybrid
  • Gene cloning and gene editing (e.g. CRISPR/Cas)
  • Protoplast isolation and transformation
  • Agrobacterium mediated genetic transformation
  • Particle bombardment for plant transformation
  • Proteomic analysis (e.g. SDS-PAGE, 2-DE)
  • In vitro molecular analysis (e.g. Pull-down, Co-IP)
  • Imaging techniques (e.g. BiFC)
  • Molecular cytogenetics (e.g. FISH)
  • Plant physiology
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