Basic course on NMR spectroscopy
A basic course on NMR spectroscopy will take place at ITQB from June 17 to 19. Organized by CERMAX, this three day course includes both theoretical and practical sessions covering basic aspects of the technique and oriented towards both beginners and routine NMR users.
Integrated in the National NMR Network Facility, CERMAX - Centro de Ressonância Magnética Nuclear António Xavier - has several NMR spectrometers (300, 400, 500 and 800 MHz), including the highest field spectrometer in Portugal.
The course topics include basic theoretical aspects of NMR, introduction to the most commonly used 1D and 2D homonuclear and heteronuclear techniques, data acquisition and processing with a “hands-on” approach.
Applications should be sent by e-mail to lamosa@itqb.unl.pt stating your interest and accompanied by a short CV. For PhD students the course will correspond to 1 ECTS (send your applications until 3 of June).
The course is open and free of charge, however the practical sessions are restricted to 12 students.
Program
17th of June
9.00 – Introduction to NMR spectroscopy (the phenomenon, basic properties of the signal).
10.00 – Instrumental aspects of the spectrometer.
10.30 – Break
11.00 – Introduction to TopSpin software.
13.00 – Lunch break
14.30 – Practical sessions
Sample preparation
Acquisition (1D, presat, APT)
Processing and analyzing 1D
18th of June
9.00 – Practical aspects of acquisition and processing (acquisition parameters, pulse programs, 90º pulse and T1 determination).
10.00 – The Nuclear Overhauser Effect
10.30 – Break
11.00 – Introduction to 2D NMR spectroscopy (Homonuclear correlation)
12.00 – INEPT, HSQC and HMBC
13.00 – Lunch break
14.30 – Practical sessions
Acquisition (COSY, HSQC)
Processing and analyzing 2D
19th of June
9.00 – DOSY.
9.45 – Protein structure
10.30 – Break
11.00 – Assignment strategies in small molecules (tutorial and exercises)
13.00 – Lunch break
14.30 – Practical sessions
Acquisition exercises
Faculty:
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