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[Course] Fundamentals of light microscopy and image processing

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When 18 Sep, 2023 12:00 AM to
29 Sep, 2023 12:00 AM
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Short course

ITQB NOVA is a reference in fluorescence microscopy in the context of microbiology and infection biology, possessing the technical knowledge and equipment to provide excellent training to researchers in the life sciences. ITQB NOVA, through the Bacterial Imaging Cluster (BIC), has specialists in different advanced microscopy techniques and preparation of complex samples, as well as conventional and super-resolution microscopy systems in biosafety. This potential for knowledge creation and sharing is further enhanced by the strong integration of ITQB NOVA in the Portuguese bioimaging community.

The course "Fundamentals of light microscopy and image processing" will focus on the application of fluorescence microscopy in molecular and cellular microbiology and infection biology. This course will pass on basic concepts and practical training in conventional epifluorescence and confocal microscopy, super-resolution and electron microscopy. Trainees will learn principles of sample preparation and advanced applications in fluorescence microscopy (FRET, FLIM and FRAP). Through a combination of theoretical presentations and interactive coding sessions, trainees will learn methods of image analysis and machine learning. These concepts and tools will be applied in small group projects in interaction with the trainers.

Credits: 2 ECTS 


Target audience

Anyone interested in fluorescence microscopy with a background in life sciences-related disciplines, including MSc or PhD students, PhD researchers, scientific research technicians, etc.

Fellowships are available for eligible students under the PRR regulations (see Official Notice for full details)

Number of participants



From 19 JUN to 02 AUG 2023

Registration fee: 25€

Tuition fee: 930 €*

* - Fellowships are available for eligible students, under the PRR regulations (see Official Notice).

Course Dates

From 18 SEP to 29 SEP 2023

Location: ITQB NOVA


Official Announcement


Pedro Matos Pereira [Bacterial Imaging Cluster (BIC)]

Mónica Serrano [Bacterial Imaging Cluster (BIC)]

Carolina Feliciano [Bacterial Imaging Cluster (BIC)]

This course is supported by European funds under the Recovery and Resilience Plan (PRR) of the Portuguese Republic, following the approval of the "CIVIC and GLOBAL" project of Universidade NOVA de Lisboa (RE - C06.i03.03 - Impulso Adultos), through fellowhips for elegible students under the PRR regulations.

The Recovery and Resilience Plan is a nationally applicable programme. The Plan will implement a set of reforms and investments aimed at restoring sustained economic growth, supporting the goal of convergence with Europe over the next decade.

The European Union, faced with the serious impacts of the pandemic on European economies, has created Next Generation EU, a strategic instrument to mitigate the economic and social impact of the crisis; this is capable of promoting economic convergence and resilience, helping to ensure long-term sustainable growth and meeting the challenges of the transition to a greener and more digital society. The Recovery and Resilience Mechanism has been developed on the basis of this instrument, and the RRP fits into this framework.

The RRP is an investment plan for all Portuguese people, based on three structuring dimensions: Resilience; Climate Change; Digital Transition.

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