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Tiago N. Cordeiro Lab

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The Dynamic Structural Biology Lab explores the role of structural disorder in biological phenomena and human health.

 

 

Tiago N. Cordeiro
Investigador Auxiliar
PhD in 2012, University of Barcelona, Spain

Phone (+351) 214469313
Extension 1313
tiago.cordeiro@itqb.unl.pt

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Research Interests

Research in the Dynamic Structural Biology (DSB) lab is focused on  discovering the underlying principles of protein disorder in biology and disease. To this end, we employ nuclear magnetic resonance (NMR) and solution small-angle scattering (X-rays and neutrons, i.e. SAXS and SANS) to provide unique and alternative insights into structural dynamics and interactions of structurally disordered proteins underlying key biological processes, such as bacterial pathogenesis and chronic infections. We are interested in defining the molecular mechanisms by which pathogenic effectors use intrinsic disorder, motif mimicry and multivalency, to modulate and hijack host cell networks to their advantage, with ultimate aim to aid the development of novel virulence-targeted antimicrobials and tools for interrogating eukaryotic cell processes.

The current and forthcoming research projects also aim:

  1. to develop methods to probe local and long-range structure in intrinsically disordered proteins (IDPs)
  2. to  bring insights into multi-domain proteins in which structural disorder give rise to extremely complex conformational energy landscape, and the role this plays in function and disease;
  3. to disentangle disordered macromolecular assemblies that are linked to cell signalling and regulation.
  4. Ultimately, provide dynamic structural information on membrane proteins under native conditions.

The central focus is to exploit the synergistic potential of NMR and SAXS/SANS as an integrated platform to map structural ensembles and dynamics of flexible bimolecular systems that are hard or impossible to crystallise.

 

Group Members

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Selected Publications

  1. Cordeiro T.N., Herranz-Trillo F., Urbanek A., Estaña A., Corttés, Sibille N., Bernardó P. Small-Angle Scattering studies of Intrinsically Disordered Proteins and their Complexes. Curr. Opin. Struct. Biol. (2017) 42,15-23

  2. Cordeiro T.N., Chen P-C., De Biasio A., Sibille N., Blanco F.J., Hub J.S., Crehuet R., Bernadó P. Disentangling polydispersity in PCNA-p15PAF complex, a disordered, transient and multivalent macromolecular assembly. Nucleic Acids Res.  (2016) doi: 10.1093/nar/gkw1183

  3. Cordeiro T.N., García J., Bernardo P., Millet O., Pons M. A Three-protein charge zipper stabilizes a complex modulating Bacterial Gene Silencing. J Biol Chem (2015) 290, 21200–12 

Laboratory's Website

For further information please visit the laboratory's website (under construction)

 

Biologia Estrutural Dinâmica (PT)

Existe um grupo de proteínas denominado "Proteínas Intrinsecamente Desordenadas" (IDPs), que têm papéis essenciais em todos os organismos vivos representando mais de 30% do proteoma humano. Dada a sua importância em quase todos os processos de sinalização celular, as IDPs disfuncionais estão na origem de várias doenças como o cancro e doenças neurodegenerativas. As IDPs são também centrais nas doenças de origem microbiana, já que certos patogéneos produzem IDPs capazes de interferir e alterar os processos internos do hospedeiro para evadir a resposta imune e promover infeção e colonização. O laboratório de Biologia Estrutural Dinâmica dedica-se ao estudo de IDPs biologicamente relevantes, em especial as que estão envolvidas em interações patógeneo-hospedeiro. Faz uso de técnicas de Ressonância Magnética Nuclear (RMN) e Dispersão de Raio-X de Baixo Ângulo (SAXS) para estudar a estrutura das proteínas e a interacção entre biomoléculas.

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