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Booking

The Green-it research unit has a series of equipment for plant phenotyping available for use by all its members.

To book a device, please complete the booking form link. Note that the booking is only confirmed once the form has been submitted and approved. If you belong to ITQB NOVA, you may check equipment availability before booking via the provided link.

Eventual maintenance or repairs will be supported by users based on the time of use of the infrastructure.

Important guidelines:

  • Training is mandatory for all users before operating any devices.
  • A logbook must be completed during the use of each device.

 

For any questions, please reach out: Ana Fortunato (anasofia@itqb.unl.pt), Omar Vergara (vergara@itqb.unl.pt) or Tiago Lourenço (tsantos@itqb.unl.pt).

 

Equipment’s List

Portable Photosynthesis System LI-6800 (LI-COR)

The Portable Photosynthesis System simultaneously measures photosynthetic gas exchange and chlorophyll a fluorescence over the same sample area, providing detailed insights into photobiology. It supports a variety of sample types, including soil and insects, enabling diverse experimental applications. Controlled environmental conditions ensure repeatable and reliable results. The system quantifies photosynthesis (An), stomatal conductance (gs), transpiration (Tr), internal to atmospheric CO₂ ratio (Ci/Ca), electron transport rate (ETR), photosystem II efficiencies (Fv’/Fm’, Fq’/Fm’), fraction of open PSII centers (qL), water use efficiency (WUE), respiration (R), and can generate An-Ci and An-PAR response curves for comprehensive plant physiological analysis.

Extra chambers are available (small plant chamber and large leaf chamber)

Two devices (LICOR 1 and LICOR 2) are currently available for use. LICOR 2 is primarily designated for use within ITQB facilities, while LICOR 1 is mainly for outdoor and field applications.

Portable Photosynthesis System LI-6400/XT (LI-COR)

The Portable Photosynthesis System LI-6400/XT is a compact and highly accurate device designed for measuring photosynthesis, respiration, and fluorescence in plants. It features integrated CO2 and H2O analyzers located directly in the sensor head, enabling real-time and precise gas exchange measurements. The system allows precise control of environmental factors such as CO2 concentration, humidity, temperature, and light intensity, replicating various experimental conditions. Its interchangeable leaf chambers and computer-controlled LED or RGB light sources provide flexibility for different plant types and research needs.

Fluorometer MultispeQ v2.0 (PhotosynQ)

The MultispeQ is a cost-effective, customizable handheld device that combines a fluorometer, chlorophyll meter, and spectrometer for field applications. It measures photosynthetic phenotypes under natural conditions and helps identify biotic and abiotic plant or algae stresses, with data collection supported through the global PhotosynQ network. Key measurements include fluorescence parameters (qL, qP, ΦII, LEF, NPQ), absorbance parameters (vH+, gH+, ECSt, relative chlorophyll content), and environmental factors (temperature, humidity, barometric pressure, leaf temperature, light intensity, cardinal direction, and tilt). It is ideal for research requiring both precision and versatility.

Two devices (MultispeQ 1 and MultispeQ 2) are currently available for use.

Thermal Camera Ti401 PRO (Fluke)

This advanced thermal camera delivers in-focus images in seconds, thanks to its LaserSharp™ Auto Focus technology. Equipped with a built-in laser distance meter, it calculates and displays the target distance, ensuring rapid and accurate focus adjustments. Ideal for measuring canopy, plant, or organ temperatures. With a high-resolution 640 x 480 thermal imaging capability, the camera reveals fine details and helps detect anomalies faster.

  

Spectroradiometer FieldSpec 4 Standard-Res (ASD)

The spectroradiometer measures plant or organ reflectance across a wide range of 350 to 25,000 nm and can calculate hundreds of vegetation indices linked to plant growth and health. Featuring 3 nm VNIR resolution and 10 nm SWIR resolution, is ideal for diverse applications, including the study of vegetation, water bodies, soils, minerals, pollution, etc.

  

Drone DJI Mavic 2 Pro and AgroCam NDVI camera

Drone for RGB imaging. Calculation of several vegetation indices from RGB images.

Possibility to measure also NDVI index using the AgroCam NDVI camera.

(This equipment requires certification in drone flight)

Drone DJI Matrice 300 RTK with MicaSense Altum camera

Drone with a camera for high resolution RGB, thermal and multispectral (5 bands) imaging

(This equipment requires certification in drone flight)

    

PlantScreen™ Modular System for Semi-automated  phenotyping of Plants (PSI)

High-throughput, semi-automated, indoor phenotyping system, including 3 modules:

  • High-Sensitivity Kinetic Chlorophyll Fluorescence Imaging Unit
  • RGB Structural Imaging Unit
  • VNIR Hyperspectral Imaging Unit

 

(Available soon!)

CanoScan LiDE 400 (CANON)

The CanoScan LiDE 400 scanner by Canon is an efficient tool for capturing detailed scans of leaves or entire plants. It is well-suited for performing color and area analyses, providing accurate and high-resolution results for applications in plant research and related studies.

   

Digital camera ILCE6000 (SONY)

The Sony ILCE6000 camera is designed for the acquisition of high-quality RGB images, making it an excellent tool for plant and leaf phenotype analysis. It enables the calculation of vegetation indices such as biomass, green area, senescence index, and plant height, offering precise and reliable data for comparative studies in plant science and related fields