2022

Simao D, Gomes CM, Alves PM, Brito C. Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models. Biotechnology Advances. 2022. 55: 107883.  doi:10.1016/j.biotechadv.2021.107883

Mendes J P, Bergman M, Solbrand A, Peixoto C, Carrondo MJT, Silva RJS. Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography. Pharmaceutics. 2022. 14(7): 1346. doi: 10.3390/pharmaceutics14071346

Mendes JP, Fernandes B, Pineda E, Kudugunti S, Bransby M, Gantier R, Peixoto C, Alves PM, Roldão A, Silva RJS. AAV process intensification by perfusion bioreaction and integrated clarification. Frontiers in Bioengineering and Biotechnology. 2022. 10: 1020174. doi:10.3389/fbioe.2022.1020174

Louro AF, Virgolini N, Paiva MA, Isidro IA, Alves PM, Gomes‑Alves P, Serra M. Expression of Extracellular Vesicle PIWI‑Interacting RNAs Throughout hiPSC‑Cardiomyocyte Differentiation. Frontiers in Physiology. 2022. 13: 926528. doi:10.3389/fphys.2022.926528

Fernandes B, Correia R, Alves PM, Roldão A. Intensifying Continuous Production of Gag‑HA VLPs at High Cell Density Using Stable Insect Cells Adapted to Low Culture Temperature. Frontiers in Bioengineering and Biotechnology. 2022. 10: 917746. doi:10.3389/fbioe.2022.917746

Correia R, Fernandes B, Castro R, Nagaoka H, Takashima E, Tsuboi T, Fukushima A, Viebig NK, Depraetere H, Alves PM, Roldão A. Asexual Blood‑Stage Malaria Vaccine Candidate PfRipr5: Enhanced Production in Insect Cells. Frontiers in Bioengineering and Biotechnology. 2022. 10: 908509. doi:10.3389/fbioe.2022.908509

Moita MR, Silva MM, Diniz C, Serra M, Hoet RM, Barbas A, Simao D. Transcriptome and proteome profiling of activated cardiac fibroblasts supports target prioritization in cardiac fibrosis. Frontiers in Cardiovascular Medicine. 2022. 9: 1015473. doi:10.3389/fcvm.2022.1015473

Sebastião MJ, Almeida HV, Serra M, Hamdani N, Saraiva F, Lourenço AP, Barros AS, Vasques‑Nóvoa F, Leite‑Moreira A, Alves PM, Falcão‑Pires I, Gomes‑Alves P. Unveiling Human Proteome Signatures of Heart Failure with Preserved Ejection Fraction. Biomedicines. 2022. 10(11): 2943. doi:10.3390/biomedicines10112943

Fernandes RP, Escandell JM, Guerreiro ACL, Moura F, Faria TQ, Carvalho SB, Silva RJS, Gomes‑Alves P, Peixoto C. Assessing multi‑attribute characterization of enveloped and non‑enveloped viral particles by capillary electrophoresis. Viruses. 2022. 14(11): 2539. doi:10.3390/v14112539 

Silvano M, Correia R, Virgolini N, Clarke C, Alves PM, Isidro IA, Roldão A. Gene Expression Analysis of Adapted Insect Cells during Influenza VLP Production Using RNA‑Sequencing. Viruses. 2022. 14(10): 2238. doi:10.3390/v14102238

Carvalho SB, Dias MM, Matheise JP, Knott I, Gomes‑Alves P, Alves PM. Biolayer Interferometry Analysis for a Higher Throughput Quantification of In‑Process Samples of a Rotavirus Vaccine. Vaccines (Basel). 2022. 10(10): 1585. doi:10.3390/vaccines10101585

Mendes R, Graça G, Silva F, Guerreiro ACL, Gomes‑Alves P, Serpa J, Boghaert ER, Alves PM, Félix A, Brito C, Isidro IA. Exploring Metabolic Signatures of Ex Vivo Tumor Tissue Cultures for Prediction of Chemosensitivity in Ovarian Cancer. Cancers (Basel). 2022. 14(18): 4460. doi:10.3390/cancers14184460

Domenici G, Trindade G, Estrada MF, Cartaxo AL, Alves PM, André S, Brito C. Patient‑Derived Breast Cancer Tissue Cultures for Anti‑Endocrine Drug Assays. Methods in Molecular Biology. 2022. 2535:‑11‑31. doi:10.1007/978‑1‑0716‑2513‑2_2

Moura‑Sampaio J, Faustino AF, Boeuf R, Antunes MA, Ewert S, Batista AP. Reconstruction of full antibody sequences in NGS datasets and accurate VL:VH coupling by cluster coordinate matching of non‑overlapping reads. Computational and Structural Biotechnology Journal. 2022 May 31;20:2723–2727. doi:10.1016/j.csbj.2022.05.054

Fernandes B, Sousa M, Castro R, Schäfer J, Hauser H, Schulze J, Amacker M, Tamborrini M, Plushke G, Alves PM, Fleury MJ, Roldão A. Scalable process for high‑yield production of PfCyRPA using insect cells for inclusion in a malaria virosome‑based vaccine candidate. Frontiers in Bioengineering and Biotechnology. 2022. 10: 879078. doi:10.3389/fbioe.2022.879078

Fernandes B, Castro R, Bhoelan F, Bemelman D, Gomes R, Costa J, Gomes‑Alves P, Stegmann T, Amacker M, Alves PM, Fleury S, Roldão A. Insect Cells for High‑Yield Production of SARS‑CoV‑2 Spike Protein: Building a Virosome‑Based COVID‑19 Vaccine Candidate. Pharmaceutics. 2022 Apr 13; 14(4): 854. doi: 10.3390/pharmaceutics14040854

Carvalho SB, Silva RJS, Sousa MFQ, Peixoto C, Roldão A, Carrondo MJT, Alves PM. Bioanalytics for Influenza Virus‑Like Particle Characterization and Process Monitoring. Frontiers in Bioengineering and Biotechnology. 2022. 10: 805176. doi:10.3389/fbioe.2022.805176

Fontinha D, Arez F, Ramella Gal I, Nogueira G, Moita D, Baeurle THH, Brito C, Spangenberg T, Alves PM, Prudêncio M. Pre-erythrocytic Activity of M5717 in Monotherapy and Combination in Preclinical Plasmodium Infection Models. ACS Infectious Diseases. 2022 Apr 8; 8(4): 721–727. doi:10.1021/acsinfecdis.1c00640

Carvalho SB, Gomes RA, Pfenninger A, Fischer M, Strotbek M, Isidro IA, Tugçu N, Gomes‑Alves P. Multi‑attribute method implementation using a High‑Resolution Mass Spectrometry platform: From sample preparation to batch analysis. PLoS One. 2022 Jan 27; 17(1): e0262711. doi:10.1371/journal.pone.0262711

Escandell JM, Pais D Am, Carvalho SB, Vincent K, Gomes‑Alves P, Alves PM. Leveraging rAAV bioprocess understanding and next generation bioanalytics development. Current Opinion in Biotechnology. 2022 Apr; 74: 271–277. doi:10.1016/j.copbio.2021.12.009

Almeida JI, Tenreiro MF, Martinez‑Santamaria L, Guerrero‑Aspizua S, Gisbert JP, Alves PM, Serra M, Baptista PM. Hallmarks of the human intestinal microbiome on liver maturation and function. Journal of Hepatology. 2022 Mar; 76(3): 694–725. doi:10.1016/j.jhep.2021.10.015