SOR
Electron density distribution and
geometry in the active site of mono-iron, non-hemic
proteins can
be characterized from the RR spectra.
Superoxide
reductases are enzymes involved in combating oxidative stress by
depleting superoxide levels in anaerobic and microaerobic prokaryotes.
The RR spectra of SORs from different sources and especially designed
mutant proteins can indicate the features responsible for different
catalytic and ligand binding properties in these enzymes.
The
electronic properties of the Fe-S bond, revealed through the
intensity weighted frequency, <νFe-S>,
play a crucial role in reduction of superoxide and product dissociation
in SORs.