 Blue copper proteins
CotA
RR
spectroscopy is a powerful tool for
investigating the coordination geometry of the Cu2+
in the T1 site of multicopper oxygen reductases. By using the charge
transfer excitation, an estimation of the Cu-S(Cys) bond length can be
extracted from the RR spectra, which has a direct correlation with
standard enthalpy of Cu2+ reduction, redox
potential of T1 site and other thermodynamic parameters in laccases.
We use RR spectroscopy in order to
understand the role of the Cu-S
bond strength in controlling the spectroscopic, electrochemical and
catalytic features of the T1 copper site of CotA and specially designed
mutants with tuned electrochemical features,
which possess potentially interesting and diverse biotechnological
applications.
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