When
energy of the incident laser light coincides with the energy of
electronic transition of the molecule, a resonance condition is
achieved. Resonance Raman spectra have increased sensitivity and
contain vibrational modes of the chromophore only, revealing structural
information on this specific site in a metalloprotein, enzyme or a
model compound.
UV-Vis
spectra of cbb3 oxygen reductase in the oxidized
and reduced
state, both in resonance with the 413 nm laser line.
