Plant Cell Biology Lab
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Rita Abranches |
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| Principal Investigator | ||
| ritaa@itqb.unl.pt | ||
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Current members |
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| Bárbara Rebelo | PhD student |
| Daniela Simões | MSc |
| Rita Pires | MSc |
| Ana Clara Ferreira | MSc student |
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Collaborators |
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| Andreas Schiermeyer | Fraunhofer IME, Aachen, Germany |
| Renier van der Hoorn | University of Oxford, UK |
| Rita Ventura | ITQB NOVA, Portugal |
| Sérgio Filipe | FCT NOVA, Portugal |
| Clélia Afonso | IPL, MARE, Peniche, Portugal |
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Current Projects |
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2018-2022 | "Aspartic Proteases from Cynara cardunculus L.: study of gene expression and establishment of alternative production platforms" (PTDC/BAA-AGR/30447/2017) Coordinator: Rita Abranches |
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| Biofactories for high value natural pigments: the production of carotenoids in plant cell cultures. First edition of the InnoValley Proof of Concept Fund |
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Past Projects |
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| 2017-2021 | "Impact of Nuclear Domains On Gene Expression and Plant Traits” (COST Action CA16212) Management Committee Member: Rita Abranches | |
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2013-2016 | "PRODuCE: Tailor-made expression hosts depleted in protease activity for recombinant protein production” (ERA-NET Project ERA-IB-2) Portuguese coordinator: Rita Abranches |
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2013-2015 | "Epigenetic modulation of transgene expression in plant cell cultures for the improved production of recombinant proteins" (PTDC/BIA-PLA/2411/2012) Coordinator: Rita Abranches |
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2013-2015 | "Plant strategies for detection of the inflamatory bacterial peptidoglycan molecule" (PTDC/BIA-PLA/3432/2012) Coordinator: Sérgio Filipe |
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2012-2015 | "Implications of uncertain transgene insertion in maize: effect in protein-coding and non-coding regulatory genes" (PTDC/AGR-GPL/121536/2010) Coordinator: Eugénia Andrade |
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| 2008-2013 | "Molecular farming: plants as a production platform for high value proteins" (COST Action FA0804) National delegate and Management Committee Member: Rita Abranches | |
| 2011-2014 | "The pathogen’s perspective of molecular plant-microbe interactions: genes expressed during the infection process of coffee leaf rust - Hemileia vastatrix" (PTDC/AGR-GPL/114949/2009) Coordinator: Pedro Talhinhas | |
| 2011 | "Investigating the subcellular trafficking of human recombinant proteins expressed in plants" (Interbio Collaborative Project 2011) Coordinators: Rita Abranches (ITQB), José María Seguí (Universitat Politecnica de Valencia) | |
| 2008-2011 | "Chromatin remodelling and abiotic stress responses in rice” (PTDC/BIA-BCM/64215/2006) Coordinator: Ana Paula Santos (Plant Genetic Engineering Lab, ITQB) | |
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2005-2007 | "Transgenic plants as models to study regulation of transgene expression and recombinant protein deposition" (POCTI/BIA-BCM/55762/2004) Coordinator: Rita Abranches |
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Main Publications |
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| 2022 | |
| AFONSO C, Bragança AR, Rebelo BA, Serra TS, Abranches R (2022) Optimal Nitrate Supplementation in Phaeodactylum tricornutum Culture Medium Increases Biomass and Fucoxanthin Production. Foods,11(4). doi: 10.3390/foods11040568 | |
| REBELO BA, Folgado A, Ferreira AC, Abranches R (2022) Production of the SARS-CoV-2 Spike protein and its Receptor Binding Domain in plant cell suspension cultures. Frontiers in Plant Science,13. doi: 10.3389/fpls.2022.995429 | |
| 2021 | |
| FOLGADO A, Abranches R (2021) Tobacco BY2 cells expressing recombinant cardosin B as an alternative for production of active milk clotting enzymes. Scientific Reports, 11(1), pp.1-11. DOI: 10.1038/s41598-021-93882-6 | |
| LOBATO M, Huang X, Alvarez D, He W, Baysal C, Zhu C, Armario-Najera V,Blanco Perera A, Cerda P, Saba A, Sobrino GM, Abranches R, Abreu IA, Balamurugan S, Bock R, Buyel JF, da Cunha NB, Daniel H, Faller R, Folgado A, Gowtham I, Häkkinen ST, Khan I, Kumar RS, Lacorte C, Lomonossoff GP, Luís IM, Ma JKC, McDonald KA, Murad A, Nandi S, O’Keef B, Oksman-Caldentey K-M, Parthiban S, Paul MJ, Ponndorf D, Rech E, Rodrigues JCM, Ruf S, Schillberg S, Schwestka J, Shah PS, Singh R, Stoger E, Twyman RM, Varghese IP. Vianna GR, Webster G, Wilbers RHP, Capell T, Christou P (2021) Contributions of the international plant science community to the fight against infectious diseases in humans – part 1: epidemic and pandemic diseases, including HIV/AIDS and coronaviruses. Plant biotechnology journal, 19(10), pp.1901-1920. DOI: 10.1111/pbi.13657 | |
| HE W, Lobato M, Huang X, Alvarez D, Baysal C, Zhu C, Armario-Najera V,Blanco Perera A, Cerda P, Saba A, Sobrino GM, Abranches R, Abreu IA, Balamurugan S, Bock R, Buyel JF, da Cunha NB, Daniel H, Faller R, Folgado A, Gowtham I, Häkkinen ST, Khan I, Kumar RS, Lacorte C, Lomonossoff GP, Luís IM, Ma JKC, McDonald KA, Murad A, Nandi S, O’Keef B, Oksman-Caldentey K-M, Parthiban S, Paul MJ, Ponndorf D, Rech E, Rodrigues JCM, Ruf S, Schillberg S, Schwestka J, Shah PS, Singh R, Stoger E, Twyman RM, Varghese IP. Vianna GR, Webster G, Wilbers RHP, Capell T, Christou P (2021) Contributions of the international plant science community to the fight against infectious diseases in humans –part 2: endemic and re-emerging diseases. Plant biotechnology journal, 19(10), pp.1921-1936. DOI: 10.1111/pbi.13658 | |
| FOLGADO A, Serra AT, Prazeres I, Bento-Silva A, Bronze MR, Abranches R (2021) Hairy root cultures of Cynara cardunculus L. as a valuable source of hydroxycinnamic acid compounds. Plant Cell, Tissue and Organ Culture (PCTOC), pp.1-11. DOI: 10.1007/s11240-021-02102-6 | |
| Other Selected Publications | |
| REBELO BA, Farrona S, Ventura MR, Abranches R (2020) Canthaxanthin, a Red-Hot Carotenoid: Applications, Synthesis, and Biosynthetic Evolution. Plants 9, 1039.1039; 1039. DOI: 10.3390/plants9081039 | |
| FOLGADO A, Abranches R (2020) Plant Aspartic Proteases for Industrial Applications: Thistle Get Better. Plants 9, 147. DOI:10.3390/plants9020147 | |
| REBELO B, Santos RB, Ascenso O, Nogueira AC, Lousa D, Abranches R, Ventura MR (2020) Synthesis and biological effects of small molecule enhancers for improved recombinant protein production in plant cell cultures. Bioorganic Chemistry 94, 103452. DOI: 10.1016/j.bioorg.2019.103452 | |
| FOLGADO A, Pires AS, Figueiredo AC, Pimentel C, Abranches R (2020) Toward alternative sources of milk coagulants for cheese manufacturing: establishment of hairy roots culture and protease characterization from Cynara cardunculus L. Plant Cell Reports 39, 89–100. DOI: 10.1007/s00299-019-02475-1. | |
| FOLGADO A, Abranches R (2019) O cardo e a indústria de queijo: ferramentas biotecnológicas para a produção de enzimas utilizadas na coagulação do leite. Revista de Ciências Agrárias 42, 817-828. DOI: 10.19084/rca.17565 | |
| SANTOS RB, Pires AS, van der Hoorn RAL, Schiermeyer A, Abranches R (2019) Generation of transgenic cell suspension cultures of the model legume Medicago truncatula: A rapid method for Agrobacterium mediated gene transfer. Plant Cell, Tissue and Organ Culture (PCTOC) 136, 445-450. DOI:10.1007/s11240-018-1525-3 | |
| SANTOS RB, Chandra B, Mandal MK, Kaschani F, Kaiser M, Both L, van der Hoorn RAL, Schiermeyer A, Abranches R (2018) Low protease content in Medicago truncatula cell cultures facilitates recombinant protein production. Biotechnology Journal 13(7):e1800050. DOI: 10.1002/biot.201800050. | |
| SANTOS RB, Pires AS, Abranches R (2017) Addition of a histone deacetylase inhibitor increases recombinant protein expression in Medicago truncatula cell cultures. Scientific Reports 7: 16756. DOI:10.1038/s41598-017-17006-9 | |
| SANTOS RB, Abranches R, Fischer R, Sack M, Holland T (2016) Putting the spotlight back on plant suspension cultures. Frontiers in Plant Science 7:297. DOI: 10.3389/fpls.2016.00297 | |
| ROSA S, Ntoukakis V, Ohmido N, Pendle A, Abranches R, Shaw P (2014) Cell differentiation and development in Arabidopsis are associated with changes in histone dynamics at the single cell level. The Plant Cell 26: 4821–4833. DOI: 10.1105/tpc.114.133793 | |
