Plant Reproduction and Evolution Lab
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Jörg Becker |
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| Principal Investigator | ||
| jbecker@itqb.unl.pt | ||
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Plant Reproduction and Evolution
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Current members |
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| María Flores-Tornero | Post-Doc Researcher |
| Helena Sapeta | Post-Doc Researcher |
| Diogo Lucas | PhD student |
| Anton Kermanov | PhD student |
| Armin Horn | PhD student |
| Mariana Vendeirinho | MSc student |
| Amadeus Pendl | MSc student |
| Miguel Sordo | MSc student |
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Collaborators |
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| Isabel Abreu | ITQB NOVA |
| Paula Duque | IGC |
| David Honys | Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic |
| Xiao-Ning Zhang | St Bonaventure University, USA |
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Current Projects |
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2021-2024 | Heat stress during the progamic phase: Impact on male gametophytes of angiosperms at transcriptome and proteome level (PTDC/ASP-PLA/2007/2020) Principal Investigator: Jorg Becker |
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2022-2024 | MSCA Postdoctoral fellowship “Single cell RNA-seq analysis of individual sperm cells to identify transcriptomic differences potentially involved in preferential fertilization” (Grant agreement ID: 101059247) Principal Investigators: María Flores-Tornero / Jörg Becker |
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Past Projects |
| 2018-2022 | Heterogeneous expression in isomorphic male gametes a single cell transcriptomics approach to explore preferential fertilization in Arabidopsis thaliana (PTDC/BIA-FBT/28484/2017) Principal Investigator: Jorg Becker |
| 2017-2021 | Marie Curie ITN-EID MossTech “Advanced use of mosses for biotechnological solutions” (Grant agreement ID: 765115) Coordinator: Danmarks Tekniske Universitet |
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2015-2018 | ERA-CAPS project EVOREPRO “Evolution of sexual reproduction in plants”. Project leader: Jorg Becker |
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2010-2014 | Marie Curie ITN “PLANT developmental biology: discovering the ORIGINS of form” (Grant agreement ID: 238640) Project leader: University of Oxford |
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Main selected publications |
| 2023 |
| MISRA CS, Sousa AGG, Barros PM, Kermanov A, Becker JD (2023) Cell-type-specific alternative splicing in the Arabidopsis germline. Plant Physiology 192 (1): 85-101. doi:10.1093/plphys/kiac574 |
| FLORES-TORNERO M, Becker JD* (2023) 50 years of sperm cell isolations: from structural to omic studies. Journal of Experimental Botany. doi:10.1093/jxb/erad117 |
| FLORES-TORNERO M, Sapeta H, Becker JD (2023) Improving the haploidization toolbox: Maternal factors take the stage. Molecular Plant 16 (4): 651-653. doi:10.1016/j.molp.2023.02.008 |
| 2022 |
| CORREIA RB, Almeida JM, Wyrwoll MJ, Julca I, Sobral D, Misra CS, Guilgur LG, Schuppe HC, Silva N, Prudêncio P, Nóvoa A, Leocádio AS, Bom J, Mallo M, Kliesch S, Mutwil M, Rocha LM, Tüttelmann F, Becker JD, Navarro-Costa P (2022) The conserved transcriptional program of metazoan male germ cells uncovers ancient origins of human infertility. bioRxiv. doi:10.1101/2022.03.02.482557 |
| KLODOVÁ B, Potešil D, Steinbachová L, Michailidis C, Lindner AC, Hackenberg D, Becker JD, Zdráhal Z, Twell D, Honys D (2022) Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis. Plant Reproduction. doi: 10.1007/s00497-022-00452-5 |
| WANG W, Xiong H, Cyprys P, Malka R, Flores-Tornero M, Zhao P, Peng X, Sprunck S, Sun MX (2022) DMP8 and 9 regulate HAP2/GCS1 trafficking for the timely acquisition of sperm fusion competence. Proceedings of the National Academy of Sciences of the United States of America, 119(45), e2207608119. doi:10.1073/pnas.2207608119 |
| 2021 |
| PEREIRA SG*, Sousa AL, Nabais C, Paixão T, Holmes AJ, Schorb M, Goshima G, Tranfield EM, Becker JD*, Bettencourt-Dias M* (2021). The 3D architecture and molecular foundations of de novo centriole assembly via bicentrioles. Current Biology, 31(19), pp.4340-4353. DOI: 10.1016/j.cub.2021.07.063 |
| JULCA I, Flores-Tornero M, Proost S, Lindner A-C, Hackenberg D, Steinbachova L, Michaelidis C, Pereira SG, Misra CS, Kawashima T, Borg M, Berger F, Goldberg J, Johnson M, Honys D, Twell D, Sprunck S, Dresselhaus T, Becker JD*, Mutwil M* (2021). Comparative transcriptomic analysis reveals conserved transcriptional programs underpinning organogenesis and reproduction in land plants. Nature Plants, 7: 1143–1159. DOI: 10.1038/s41477-021-00958-2 |
| HORN A, Pascal A, Lončarević I, Volpatto Marques R, Lu Y, Miguel S, Bourgard F, Thorsteinsdóttir M, Cronberg N Becker JD, Reski R, Simonsen HT (2021). Natural products from bryophytes: from basic biology to biotechnological applications. Critical Reviews in Plant Sciences, 1-27. DOI: 10.1080/07352689.2021.1911034 |
| Other Selected Publications |
| PEREIRA PA, Boavida LC, Santos MR & Becker JD*(2020) AtNOT1 is required for gametophyte development in Arabidopsis. Plant Journal, 103: 1289–1303. DOI: 10.1111/tpj.14801 |
| BORG M, Jacob Y, Suzaki D, LeBlanc C, Buendia D, Axelsson E, Kawashima T, Voigt P, Boavida L, Becker JD, Higashiyama T, Martienssen R, Berger F (2020) Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin. Nature Cell Biology, 22(6):621-629 DOI: 10.1038/s41556-020-0515-y |
| MISRA CS, Santos MS, Rafael-Fernandes M, Martins NP, Monteiro M, Becker JD* (2019) Transcriptomics of Arabidopsis sperm cells at single-cell resolution. Plant Reproduction, 32 (1), 29-38 DOI: 10.1007/s00497-018-00355-4 |
| ZHANG Y, Ramming A, Heinke L, Altschmied L, Slotkin R, Becker JD, Kappel C, Lenhard M (2019) The poly(A) polymerase PAPS1 interacts with the RNA-directed DNA methylation pathway in sporophyte and pollen development. Plant Journal, 99 (4): 655-672. DOI: 10.1111/tpj.14348 |
| FERRARI C, Proost S, Janowski M, Becker J, Nikoloski Z, Bhattacharya D, Price D, Tohge T, Bar-Even A, Fernie A, Stitt M, Mutwil, M (2019) Kingdom-wide comparison reveals the evolution of diurnal gene expression in Archaeplastida. Nature Communications, 10 (1): 737. DOI: 10.1038/s41467-019-08703-2 |
| ORTIZ-RAMÍREZ C, Michard E, Simon AA, Damineli DSC, Hernández-Coronado M, Becker JD, Feijó JA (2017) GLUTAMATE RECEPTOR-LIKE channels are essential for chemotaxis and reproduction in mosses. Nature 549: 91–95. DOI: 10.1038/nature23478 |
| RUPRECHT C, Proost S, Hernandez-Coronado M, Ortiz-Ramirez C, Lang D, Rensing SA, Becker JD, Vandepoele K, Mutwil M (2017) Phylogenomic analysis of gene co-expression networks reveals the evolution of functional modules. Plant Journal 90: 447–465. DOI: 10.1111/tpj.13502 |
| ROCHETA M, Coito JL, Ramos MJN, Carvalho L, Becker JD, Carbonell-Bejerano P, Amâncio S (2016) Transcriptomic comparison between two Vitis vinifera L. varieties (Trincadeira and Touriga Nacional) in abiotic stress conditions. BMC Plant Biology 16: 224. DOI: 10.1186/s12870-016-0911-4 |
| BOAVIDA LC, Becker JD* (2016) Plant Evolution: What does it take to be an egg? Current Biology 26: R527–R530. DOI: 10.1016/j.cub.2016.05.04 |
| WIBOWO A, Becker C, Marconi G, Durr J, Price J, Hagmann J, Papareddy R, Putra H, Kageyama J, Becker J, Weigel D, Gutierrez-Marcos J (2016) Hyperosmotic stress memory in Arabidopsis is mediated by distinct epigenetically labile sites in the genome and is restricted in the male germline by DNA glycosylase activity. Elife 5. DOI: 10.7554/eLife.13546 |
| ORTIZ-RAMÍREZ C, Hernandez-Coronado M, Thamm A, Catarino B, Wang M, Dolan L, Feijó JA, Becker JD* (2016) A transcriptome atlas of Physcomitrella patens provides insights into the evolution and development of land plants. Molecular Plant 9: 205–220. DOI: 10.1016/j.molp.2015.12.002 |
| MACALISTER CA, Ortiz-Ramírez C, Becker JD, Feijó JA, Lippman ZB (2016) Hydroxyproline O-arabinosyltransferase mutants oppositely alter tip growth in Arabidopsis thaliana and Physcomitrella patens. Plant Journal 85: 193–208 . DOI: 10.1111/tpj.13079 |
| JIANG H, Yi J, Boavida LC, Chen Y, Becker JD, Köhler C, McCormick S (2015) Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm. Proceedings of the National Academy of Sciences U. S. A. 112: 13378–13383. DOI: 10.1073/pnas.1510854112 |
| KAPPEL C, Trost G, Czesnick H, Ramming A, Kolbe B, Vi SL, Bispo C, Becker JD, de Moor C, Lenhard M (2015) Genome-wide analysis of PAPS1-dependent polyadenylation identifies novel roles for functionally specialized Poly(A) polymerases in Arabidopsis thaliana. PLoS Genetics 11: e1005474. DOI: 10.1371/journal.pgen.1005474 |
