 | | Methods for Biosciences II (Tools for Structural Biology)Coordinators
Maria João Romão
Carlos Frazão
Teresa Santos Silva Objectives
This course aims at offering PhD students a sound and proficient understanding of the main methodologies that support Structural Biology, in a thematic and complementary perspective, towards the analysis and understanding of structure-function relationships in biological systems. Syllabus
I. Methods for 3D structural determination of biological macromolecules:
a. X-ray Crystallography and SAXS.
b. NMR (2D and 3D)
c. Electron Microscopy (cryo-EM)
d. Comparison of structural determination methods.
II. Complementary biophysical methods:
a. Optical spectroscopies (Fluorescence, Circular Dichroism)
b. EPR
c. Mass spectrometry
d. X-ray absorption spectroscopies (XAS, EXAFS and XANES)
f. Raman and Resonance Raman
g. X-ray fluorescence
h. Computational methods Evaluation
Each student will be assessed by a seminar on a specific case study that includes the analysis of scientific articles, followed by a short oral examination. Main Bibliography - “Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology”, B. Rupp, Garland Science (2009).
- “Structural Biology; Practical NMR applications” Q. Teng, Springer Science +Business Media, Inc, NY (2005).
- Proteins: From Analytics to Structural Genomics (Hardcover) by Robert A. Meyers (Editor) , Vols. I and II, Wiley-VCH Verlag (2007).
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