Cryo-EM Seminar @ ITQB NOVA - Michael Elbaum
IMpaCT project is happy to announce our Cryo-EM seminar with our expert partner guest, Michael Elbaum from the Weizmann Institute of Science.
Date: 28 July 2022
Time: 10am Lisbon Time / 11am CET
Location: @ INL (Braga) auditorium & Webinar
3D imaging of thick biological specimens with cryo-STEM
CryoEM has sparked a revolution in structural biology with atomic-scale resolution of biological macromolecules. Another revolution is underway at the level of the biological cell. The study of beam-sensitive materials at micron thickness brings new challenges. Tomography is required, implying repeated exposures, and the conventional modality of energy-filtered, wide-field TEM is poorly suited to thick specimens. We show that STEM offers unique advantages in this realm, including dose efficiency, convenient incorporation of analytical techniques, and semi-quantitative interpretation of contrast. A quadrant detector provides additionally phase and depth contrast from single scans. The talk will survey the methods and a number of key applications.
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